Cargando…

In vivo pH measurement at the site of calcification in an octocoral

Calcareous octocorals are ecologically important calcifiers, but little is known about their biomineralization physiology, relative to scleractinian corals. Many marine calcifiers promote calcification by up-regulating pH at calcification sites against the surrounding seawater. Here, we investigated...

Descripción completa

Detalles Bibliográficos
Autores principales: Le Goff, C., Tambutté, E., Venn, A. A., Techer, N., Allemand, D., Tambutté, S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5593875/
https://www.ncbi.nlm.nih.gov/pubmed/28894174
http://dx.doi.org/10.1038/s41598-017-10348-4
_version_ 1783263112740208640
author Le Goff, C.
Tambutté, E.
Venn, A. A.
Techer, N.
Allemand, D.
Tambutté, S.
author_facet Le Goff, C.
Tambutté, E.
Venn, A. A.
Techer, N.
Allemand, D.
Tambutté, S.
author_sort Le Goff, C.
collection PubMed
description Calcareous octocorals are ecologically important calcifiers, but little is known about their biomineralization physiology, relative to scleractinian corals. Many marine calcifiers promote calcification by up-regulating pH at calcification sites against the surrounding seawater. Here, we investigated pH in the red octocoral Corallium rubrum which forms sclerites and an axial skeleton. To achieve this, we cultured microcolonies on coverslips facilitating microscopy of calcification sites of sclerites and axial skeleton. Initially we conducted extensive characterisation of the structural arrangement of biominerals and calcifying cells in context with other tissues, and then measured pH by live tissue imaging. Our results reveal that developing sclerites are enveloped by two scleroblasts and an extracellular calcifying medium of pH 7.97 ± 0.15. Similarly, axial skeleton crystals are surrounded by cells and a calcifying medium of pH 7.89 ± 0.09. In both cases, calcifying media are more alkaline compared to calcifying cells and fluids in gastrovascular canals, but importantly they are not pH up-regulated with respect to the surrounding seawater, contrary to what is observed in scleractinians. This points to a potential vulnerability of this species to decrease in seawater pH and is consistent with reports that red coral calcification is sensitive to ocean acidification.
format Online
Article
Text
id pubmed-5593875
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-55938752017-09-13 In vivo pH measurement at the site of calcification in an octocoral Le Goff, C. Tambutté, E. Venn, A. A. Techer, N. Allemand, D. Tambutté, S. Sci Rep Article Calcareous octocorals are ecologically important calcifiers, but little is known about their biomineralization physiology, relative to scleractinian corals. Many marine calcifiers promote calcification by up-regulating pH at calcification sites against the surrounding seawater. Here, we investigated pH in the red octocoral Corallium rubrum which forms sclerites and an axial skeleton. To achieve this, we cultured microcolonies on coverslips facilitating microscopy of calcification sites of sclerites and axial skeleton. Initially we conducted extensive characterisation of the structural arrangement of biominerals and calcifying cells in context with other tissues, and then measured pH by live tissue imaging. Our results reveal that developing sclerites are enveloped by two scleroblasts and an extracellular calcifying medium of pH 7.97 ± 0.15. Similarly, axial skeleton crystals are surrounded by cells and a calcifying medium of pH 7.89 ± 0.09. In both cases, calcifying media are more alkaline compared to calcifying cells and fluids in gastrovascular canals, but importantly they are not pH up-regulated with respect to the surrounding seawater, contrary to what is observed in scleractinians. This points to a potential vulnerability of this species to decrease in seawater pH and is consistent with reports that red coral calcification is sensitive to ocean acidification. Nature Publishing Group UK 2017-09-11 /pmc/articles/PMC5593875/ /pubmed/28894174 http://dx.doi.org/10.1038/s41598-017-10348-4 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Le Goff, C.
Tambutté, E.
Venn, A. A.
Techer, N.
Allemand, D.
Tambutté, S.
In vivo pH measurement at the site of calcification in an octocoral
title In vivo pH measurement at the site of calcification in an octocoral
title_full In vivo pH measurement at the site of calcification in an octocoral
title_fullStr In vivo pH measurement at the site of calcification in an octocoral
title_full_unstemmed In vivo pH measurement at the site of calcification in an octocoral
title_short In vivo pH measurement at the site of calcification in an octocoral
title_sort in vivo ph measurement at the site of calcification in an octocoral
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5593875/
https://www.ncbi.nlm.nih.gov/pubmed/28894174
http://dx.doi.org/10.1038/s41598-017-10348-4
work_keys_str_mv AT legoffc invivophmeasurementatthesiteofcalcificationinanoctocoral
AT tambuttee invivophmeasurementatthesiteofcalcificationinanoctocoral
AT vennaa invivophmeasurementatthesiteofcalcificationinanoctocoral
AT techern invivophmeasurementatthesiteofcalcificationinanoctocoral
AT allemandd invivophmeasurementatthesiteofcalcificationinanoctocoral
AT tambuttes invivophmeasurementatthesiteofcalcificationinanoctocoral