Cargando…
An embryonic system to assess direct and indirect Wnt transcriptional targets
During animal development, complex signals determine and organize a vast number of tissues using a very small number of signal transduction pathways. These developmental signaling pathways determine cell fates through a coordinated transcriptional response that remains poorly understood. The Wnt pat...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5593962/ https://www.ncbi.nlm.nih.gov/pubmed/28894169 http://dx.doi.org/10.1038/s41598-017-11519-z |
_version_ | 1783263132900130816 |
---|---|
author | Suresh, Jahnavi Harmston, Nathan Lim, Ka Keat Kaur, Prameet Jin, Helen Jingshu Lusk, Jay B. Petretto, Enrico Tolwinski, Nicholas S. |
author_facet | Suresh, Jahnavi Harmston, Nathan Lim, Ka Keat Kaur, Prameet Jin, Helen Jingshu Lusk, Jay B. Petretto, Enrico Tolwinski, Nicholas S. |
author_sort | Suresh, Jahnavi |
collection | PubMed |
description | During animal development, complex signals determine and organize a vast number of tissues using a very small number of signal transduction pathways. These developmental signaling pathways determine cell fates through a coordinated transcriptional response that remains poorly understood. The Wnt pathway is involved in a variety of these cellular functions, and its signals are transmitted in part through a β-catenin/TCF transcriptional complex. Here we report an in vivo Drosophila assay that can be used to distinguish between activation, de-repression and repression of transcriptional responses, separating upstream and downstream pathway activation and canonical/non-canonical Wnt signals in embryos. We find specific sets of genes downstream of both β-catenin and TCF with an additional group of genes regulated by Wnt, while the non-canonical Wnt4 regulates a separate cohort of genes. We correlate transcriptional changes with phenotypic outcomes of cell differentiation and embryo size, showing our model can be used to characterize developmental signaling compartmentalization in vivo. |
format | Online Article Text |
id | pubmed-5593962 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55939622017-09-13 An embryonic system to assess direct and indirect Wnt transcriptional targets Suresh, Jahnavi Harmston, Nathan Lim, Ka Keat Kaur, Prameet Jin, Helen Jingshu Lusk, Jay B. Petretto, Enrico Tolwinski, Nicholas S. Sci Rep Article During animal development, complex signals determine and organize a vast number of tissues using a very small number of signal transduction pathways. These developmental signaling pathways determine cell fates through a coordinated transcriptional response that remains poorly understood. The Wnt pathway is involved in a variety of these cellular functions, and its signals are transmitted in part through a β-catenin/TCF transcriptional complex. Here we report an in vivo Drosophila assay that can be used to distinguish between activation, de-repression and repression of transcriptional responses, separating upstream and downstream pathway activation and canonical/non-canonical Wnt signals in embryos. We find specific sets of genes downstream of both β-catenin and TCF with an additional group of genes regulated by Wnt, while the non-canonical Wnt4 regulates a separate cohort of genes. We correlate transcriptional changes with phenotypic outcomes of cell differentiation and embryo size, showing our model can be used to characterize developmental signaling compartmentalization in vivo. Nature Publishing Group UK 2017-09-11 /pmc/articles/PMC5593962/ /pubmed/28894169 http://dx.doi.org/10.1038/s41598-017-11519-z Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Suresh, Jahnavi Harmston, Nathan Lim, Ka Keat Kaur, Prameet Jin, Helen Jingshu Lusk, Jay B. Petretto, Enrico Tolwinski, Nicholas S. An embryonic system to assess direct and indirect Wnt transcriptional targets |
title | An embryonic system to assess direct and indirect Wnt transcriptional targets |
title_full | An embryonic system to assess direct and indirect Wnt transcriptional targets |
title_fullStr | An embryonic system to assess direct and indirect Wnt transcriptional targets |
title_full_unstemmed | An embryonic system to assess direct and indirect Wnt transcriptional targets |
title_short | An embryonic system to assess direct and indirect Wnt transcriptional targets |
title_sort | embryonic system to assess direct and indirect wnt transcriptional targets |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5593962/ https://www.ncbi.nlm.nih.gov/pubmed/28894169 http://dx.doi.org/10.1038/s41598-017-11519-z |
work_keys_str_mv | AT sureshjahnavi anembryonicsystemtoassessdirectandindirectwnttranscriptionaltargets AT harmstonnathan anembryonicsystemtoassessdirectandindirectwnttranscriptionaltargets AT limkakeat anembryonicsystemtoassessdirectandindirectwnttranscriptionaltargets AT kaurprameet anembryonicsystemtoassessdirectandindirectwnttranscriptionaltargets AT jinhelenjingshu anembryonicsystemtoassessdirectandindirectwnttranscriptionaltargets AT luskjayb anembryonicsystemtoassessdirectandindirectwnttranscriptionaltargets AT petrettoenrico anembryonicsystemtoassessdirectandindirectwnttranscriptionaltargets AT tolwinskinicholass anembryonicsystemtoassessdirectandindirectwnttranscriptionaltargets AT sureshjahnavi embryonicsystemtoassessdirectandindirectwnttranscriptionaltargets AT harmstonnathan embryonicsystemtoassessdirectandindirectwnttranscriptionaltargets AT limkakeat embryonicsystemtoassessdirectandindirectwnttranscriptionaltargets AT kaurprameet embryonicsystemtoassessdirectandindirectwnttranscriptionaltargets AT jinhelenjingshu embryonicsystemtoassessdirectandindirectwnttranscriptionaltargets AT luskjayb embryonicsystemtoassessdirectandindirectwnttranscriptionaltargets AT petrettoenrico embryonicsystemtoassessdirectandindirectwnttranscriptionaltargets AT tolwinskinicholass embryonicsystemtoassessdirectandindirectwnttranscriptionaltargets |