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Antennal transcriptome and expression analyses of olfactory genes in the sweetpotato weevil Cylas formicarius
The sweetpotato weevil, Cylas formicarius (Fabricius), is a serious pest of sweetpotato. Olfaction-based approaches, such as use of synthetic sex pheromones to monitor populations and the bait-and-kill method to eliminate males, have been applied successfully for population management of C. formicar...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5593998/ https://www.ncbi.nlm.nih.gov/pubmed/28894232 http://dx.doi.org/10.1038/s41598-017-11456-x |
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author | Bin, Shu-Ying Qu, Meng-Qiu Pu, Xin-Hua Wu, Zhong-Zhen Lin, Jin-Tian |
author_facet | Bin, Shu-Ying Qu, Meng-Qiu Pu, Xin-Hua Wu, Zhong-Zhen Lin, Jin-Tian |
author_sort | Bin, Shu-Ying |
collection | PubMed |
description | The sweetpotato weevil, Cylas formicarius (Fabricius), is a serious pest of sweetpotato. Olfaction-based approaches, such as use of synthetic sex pheromones to monitor populations and the bait-and-kill method to eliminate males, have been applied successfully for population management of C. formicarius. However, the molecular basis of olfaction in C. formicarius remains unknown. In this study, we produced antennal transcriptomes from males and females of C. formicarius using high-throughput sequencing to identify gene families associated with odorant detection. A total of 54 odorant receptors (ORs), 11 gustatory receptors (GRs), 15 ionotropic receptors (IRs), 3 sensory neuron membrane proteins (SNMPs), 33 odorant binding proteins (OBPs), and 12 chemosensory proteins (CSPs) were identified. Tissue-specific expression patterns revealed that all 54 ORs and 11 antennal IRs, one SNMP, and three OBPs were primarily expressed in antennae, suggesting their putative roles in olfaction. Sex-specific expression patterns of these antenna-predominant genes suggest that they have potential functions in sexual behaviors. This study provides a framework for understanding olfaction in coleopterans as well as future strategies for controlling the sweetpotato weevil pest. |
format | Online Article Text |
id | pubmed-5593998 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55939982017-09-14 Antennal transcriptome and expression analyses of olfactory genes in the sweetpotato weevil Cylas formicarius Bin, Shu-Ying Qu, Meng-Qiu Pu, Xin-Hua Wu, Zhong-Zhen Lin, Jin-Tian Sci Rep Article The sweetpotato weevil, Cylas formicarius (Fabricius), is a serious pest of sweetpotato. Olfaction-based approaches, such as use of synthetic sex pheromones to monitor populations and the bait-and-kill method to eliminate males, have been applied successfully for population management of C. formicarius. However, the molecular basis of olfaction in C. formicarius remains unknown. In this study, we produced antennal transcriptomes from males and females of C. formicarius using high-throughput sequencing to identify gene families associated with odorant detection. A total of 54 odorant receptors (ORs), 11 gustatory receptors (GRs), 15 ionotropic receptors (IRs), 3 sensory neuron membrane proteins (SNMPs), 33 odorant binding proteins (OBPs), and 12 chemosensory proteins (CSPs) were identified. Tissue-specific expression patterns revealed that all 54 ORs and 11 antennal IRs, one SNMP, and three OBPs were primarily expressed in antennae, suggesting their putative roles in olfaction. Sex-specific expression patterns of these antenna-predominant genes suggest that they have potential functions in sexual behaviors. This study provides a framework for understanding olfaction in coleopterans as well as future strategies for controlling the sweetpotato weevil pest. Nature Publishing Group UK 2017-09-11 /pmc/articles/PMC5593998/ /pubmed/28894232 http://dx.doi.org/10.1038/s41598-017-11456-x Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Bin, Shu-Ying Qu, Meng-Qiu Pu, Xin-Hua Wu, Zhong-Zhen Lin, Jin-Tian Antennal transcriptome and expression analyses of olfactory genes in the sweetpotato weevil Cylas formicarius |
title | Antennal transcriptome and expression analyses of olfactory genes in the sweetpotato weevil Cylas formicarius |
title_full | Antennal transcriptome and expression analyses of olfactory genes in the sweetpotato weevil Cylas formicarius |
title_fullStr | Antennal transcriptome and expression analyses of olfactory genes in the sweetpotato weevil Cylas formicarius |
title_full_unstemmed | Antennal transcriptome and expression analyses of olfactory genes in the sweetpotato weevil Cylas formicarius |
title_short | Antennal transcriptome and expression analyses of olfactory genes in the sweetpotato weevil Cylas formicarius |
title_sort | antennal transcriptome and expression analyses of olfactory genes in the sweetpotato weevil cylas formicarius |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5593998/ https://www.ncbi.nlm.nih.gov/pubmed/28894232 http://dx.doi.org/10.1038/s41598-017-11456-x |
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