Cargando…
Suppression of cucumber stachyose synthase gene (CsSTS) inhibits phloem loading and reduces low temperature stress tolerance
Stachyose is the main transporting sugar in phloem of Raffinose family oligosaccharides-transporting species. Stachyose synthase (STS) is a key enzyme for stachyose biosynthesis, but the gene encoding STS is poorly characterized in cucumber (Cucumis sativus L.), which is a model plant for studying s...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5594042/ https://www.ncbi.nlm.nih.gov/pubmed/28608281 http://dx.doi.org/10.1007/s11103-017-0621-9 |
_version_ | 1783263149802127360 |
---|---|
author | Lü, Jianguo Sui, Xiaolei Ma, Si Li, Xin Liu, Huan Zhang, Zhenxian |
author_facet | Lü, Jianguo Sui, Xiaolei Ma, Si Li, Xin Liu, Huan Zhang, Zhenxian |
author_sort | Lü, Jianguo |
collection | PubMed |
description | Stachyose is the main transporting sugar in phloem of Raffinose family oligosaccharides-transporting species. Stachyose synthase (STS) is a key enzyme for stachyose biosynthesis, but the gene encoding STS is poorly characterized in cucumber (Cucumis sativus L.), which is a model plant for studying stachyose metabolism and phloem function. In this research, stachyose synthase gene (CsSTS) from cucumber was isolated and its physiological functions were analyzed. CsSTS expressed mainly in the phloem of the minor veins in mature leaves and localized to companion cells. Reverse genetics with CsSTS RNAi lines revealed obviously reductions in STS activity and stachyose content along with a small amount of starch accumulation in leaves, suggesting that CsSTS is involved in phloem loading of cucumber leaves. After 6 °C low temperature stress, malondialdehyde content and electrical conductivity increased, especially in CsSTS-RNAi plants. But CsSTS expression was up-regulated, STS activity and stachyose level increased, the activities of reactive-oxygen-scavenging enzyme in cucumber seedlings improved significantly and starch accumulation reduced, especially in CsSTS-OE lines. These results demonstrate clearly that CsSTS is involved in phloem loading, carbohydrate distribution and tolerance of cucumber seedlings to low temperature stress. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11103-017-0621-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5594042 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-55940422017-09-26 Suppression of cucumber stachyose synthase gene (CsSTS) inhibits phloem loading and reduces low temperature stress tolerance Lü, Jianguo Sui, Xiaolei Ma, Si Li, Xin Liu, Huan Zhang, Zhenxian Plant Mol Biol Article Stachyose is the main transporting sugar in phloem of Raffinose family oligosaccharides-transporting species. Stachyose synthase (STS) is a key enzyme for stachyose biosynthesis, but the gene encoding STS is poorly characterized in cucumber (Cucumis sativus L.), which is a model plant for studying stachyose metabolism and phloem function. In this research, stachyose synthase gene (CsSTS) from cucumber was isolated and its physiological functions were analyzed. CsSTS expressed mainly in the phloem of the minor veins in mature leaves and localized to companion cells. Reverse genetics with CsSTS RNAi lines revealed obviously reductions in STS activity and stachyose content along with a small amount of starch accumulation in leaves, suggesting that CsSTS is involved in phloem loading of cucumber leaves. After 6 °C low temperature stress, malondialdehyde content and electrical conductivity increased, especially in CsSTS-RNAi plants. But CsSTS expression was up-regulated, STS activity and stachyose level increased, the activities of reactive-oxygen-scavenging enzyme in cucumber seedlings improved significantly and starch accumulation reduced, especially in CsSTS-OE lines. These results demonstrate clearly that CsSTS is involved in phloem loading, carbohydrate distribution and tolerance of cucumber seedlings to low temperature stress. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11103-017-0621-9) contains supplementary material, which is available to authorized users. Springer Netherlands 2017-06-12 2017 /pmc/articles/PMC5594042/ /pubmed/28608281 http://dx.doi.org/10.1007/s11103-017-0621-9 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Article Lü, Jianguo Sui, Xiaolei Ma, Si Li, Xin Liu, Huan Zhang, Zhenxian Suppression of cucumber stachyose synthase gene (CsSTS) inhibits phloem loading and reduces low temperature stress tolerance |
title | Suppression of cucumber stachyose synthase gene (CsSTS) inhibits phloem loading and reduces low temperature stress tolerance |
title_full | Suppression of cucumber stachyose synthase gene (CsSTS) inhibits phloem loading and reduces low temperature stress tolerance |
title_fullStr | Suppression of cucumber stachyose synthase gene (CsSTS) inhibits phloem loading and reduces low temperature stress tolerance |
title_full_unstemmed | Suppression of cucumber stachyose synthase gene (CsSTS) inhibits phloem loading and reduces low temperature stress tolerance |
title_short | Suppression of cucumber stachyose synthase gene (CsSTS) inhibits phloem loading and reduces low temperature stress tolerance |
title_sort | suppression of cucumber stachyose synthase gene (cssts) inhibits phloem loading and reduces low temperature stress tolerance |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5594042/ https://www.ncbi.nlm.nih.gov/pubmed/28608281 http://dx.doi.org/10.1007/s11103-017-0621-9 |
work_keys_str_mv | AT lujianguo suppressionofcucumberstachyosesynthasegenecsstsinhibitsphloemloadingandreduceslowtemperaturestresstolerance AT suixiaolei suppressionofcucumberstachyosesynthasegenecsstsinhibitsphloemloadingandreduceslowtemperaturestresstolerance AT masi suppressionofcucumberstachyosesynthasegenecsstsinhibitsphloemloadingandreduceslowtemperaturestresstolerance AT lixin suppressionofcucumberstachyosesynthasegenecsstsinhibitsphloemloadingandreduceslowtemperaturestresstolerance AT liuhuan suppressionofcucumberstachyosesynthasegenecsstsinhibitsphloemloadingandreduceslowtemperaturestresstolerance AT zhangzhenxian suppressionofcucumberstachyosesynthasegenecsstsinhibitsphloemloadingandreduceslowtemperaturestresstolerance |