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Quercetin Declines Apoptosis, Ameliorates Mitochondrial Function and Improves Retinal Ganglion Cell Survival and Function in In Vivo Model of Glaucoma in Rat and Retinal Ganglion Cell Culture In Vitro

Glaucoma is a progressive neuropathy characterized by the loss of retinal ganglion cells (RGCs). Strategies that delay or halt RGC loss have been recognized as potentially beneficial for rescuing vision in glaucoma patients. Quercetin (Qcn) is a natural and important dietary flavonoid compound, wide...

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Autores principales: Gao, Feng-Juan, Zhang, Sheng-Hai, Xu, Ping, Yang, Bo-Qi, Zhang, Rong, Cheng, Yun, Zhou, Xu-Jiao, Huang, Wan-Jing, Wang, Min, Chen, Jun-Yi, Sun, Xing-Huai, Wu, Ji-Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5594060/
https://www.ncbi.nlm.nih.gov/pubmed/28936163
http://dx.doi.org/10.3389/fnmol.2017.00285
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author Gao, Feng-Juan
Zhang, Sheng-Hai
Xu, Ping
Yang, Bo-Qi
Zhang, Rong
Cheng, Yun
Zhou, Xu-Jiao
Huang, Wan-Jing
Wang, Min
Chen, Jun-Yi
Sun, Xing-Huai
Wu, Ji-Hong
author_facet Gao, Feng-Juan
Zhang, Sheng-Hai
Xu, Ping
Yang, Bo-Qi
Zhang, Rong
Cheng, Yun
Zhou, Xu-Jiao
Huang, Wan-Jing
Wang, Min
Chen, Jun-Yi
Sun, Xing-Huai
Wu, Ji-Hong
author_sort Gao, Feng-Juan
collection PubMed
description Glaucoma is a progressive neuropathy characterized by the loss of retinal ganglion cells (RGCs). Strategies that delay or halt RGC loss have been recognized as potentially beneficial for rescuing vision in glaucoma patients. Quercetin (Qcn) is a natural and important dietary flavonoid compound, widely distributed in fruits and vegetables. Mounting evidence suggests that Qcn has numerous neuroprotective effects. However, whether Qcn exerts neuroprotective effects on RGC in glaucoma is poorly understood. In this study, we investigated the protective effect of Qcn against RGC damage in a rat chronic ocular hypertension (COHT) model in vivo and hypoxia-induced primary cultured RGC damage in vitro, and we further explored the underlying neuroprotective mechanisms. We found that Qcn not only improved RGC survival and function from a very early stage of COHT in vivo, it promoted the survival of hypoxia-treated primary cultured RGCs in vitro via ameliorating mitochondrial function and preventing mitochondria-mediated apoptosis. Our findings suggest that Qcn has direct protective effects on RGCs that are independent of lowering the intraocular pressure (IOP). Qcn may be a promising therapeutic agent for improving RGC survival and function in glaucomatous neurodegeneration.
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spelling pubmed-55940602017-09-21 Quercetin Declines Apoptosis, Ameliorates Mitochondrial Function and Improves Retinal Ganglion Cell Survival and Function in In Vivo Model of Glaucoma in Rat and Retinal Ganglion Cell Culture In Vitro Gao, Feng-Juan Zhang, Sheng-Hai Xu, Ping Yang, Bo-Qi Zhang, Rong Cheng, Yun Zhou, Xu-Jiao Huang, Wan-Jing Wang, Min Chen, Jun-Yi Sun, Xing-Huai Wu, Ji-Hong Front Mol Neurosci Neuroscience Glaucoma is a progressive neuropathy characterized by the loss of retinal ganglion cells (RGCs). Strategies that delay or halt RGC loss have been recognized as potentially beneficial for rescuing vision in glaucoma patients. Quercetin (Qcn) is a natural and important dietary flavonoid compound, widely distributed in fruits and vegetables. Mounting evidence suggests that Qcn has numerous neuroprotective effects. However, whether Qcn exerts neuroprotective effects on RGC in glaucoma is poorly understood. In this study, we investigated the protective effect of Qcn against RGC damage in a rat chronic ocular hypertension (COHT) model in vivo and hypoxia-induced primary cultured RGC damage in vitro, and we further explored the underlying neuroprotective mechanisms. We found that Qcn not only improved RGC survival and function from a very early stage of COHT in vivo, it promoted the survival of hypoxia-treated primary cultured RGCs in vitro via ameliorating mitochondrial function and preventing mitochondria-mediated apoptosis. Our findings suggest that Qcn has direct protective effects on RGCs that are independent of lowering the intraocular pressure (IOP). Qcn may be a promising therapeutic agent for improving RGC survival and function in glaucomatous neurodegeneration. Frontiers Media S.A. 2017-09-07 /pmc/articles/PMC5594060/ /pubmed/28936163 http://dx.doi.org/10.3389/fnmol.2017.00285 Text en Copyright © 2017 Gao, Zhang, Xu, Yang, Zhang, Cheng, Zhou, Huang, Wang, Chen, Sun and Wu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Gao, Feng-Juan
Zhang, Sheng-Hai
Xu, Ping
Yang, Bo-Qi
Zhang, Rong
Cheng, Yun
Zhou, Xu-Jiao
Huang, Wan-Jing
Wang, Min
Chen, Jun-Yi
Sun, Xing-Huai
Wu, Ji-Hong
Quercetin Declines Apoptosis, Ameliorates Mitochondrial Function and Improves Retinal Ganglion Cell Survival and Function in In Vivo Model of Glaucoma in Rat and Retinal Ganglion Cell Culture In Vitro
title Quercetin Declines Apoptosis, Ameliorates Mitochondrial Function and Improves Retinal Ganglion Cell Survival and Function in In Vivo Model of Glaucoma in Rat and Retinal Ganglion Cell Culture In Vitro
title_full Quercetin Declines Apoptosis, Ameliorates Mitochondrial Function and Improves Retinal Ganglion Cell Survival and Function in In Vivo Model of Glaucoma in Rat and Retinal Ganglion Cell Culture In Vitro
title_fullStr Quercetin Declines Apoptosis, Ameliorates Mitochondrial Function and Improves Retinal Ganglion Cell Survival and Function in In Vivo Model of Glaucoma in Rat and Retinal Ganglion Cell Culture In Vitro
title_full_unstemmed Quercetin Declines Apoptosis, Ameliorates Mitochondrial Function and Improves Retinal Ganglion Cell Survival and Function in In Vivo Model of Glaucoma in Rat and Retinal Ganglion Cell Culture In Vitro
title_short Quercetin Declines Apoptosis, Ameliorates Mitochondrial Function and Improves Retinal Ganglion Cell Survival and Function in In Vivo Model of Glaucoma in Rat and Retinal Ganglion Cell Culture In Vitro
title_sort quercetin declines apoptosis, ameliorates mitochondrial function and improves retinal ganglion cell survival and function in in vivo model of glaucoma in rat and retinal ganglion cell culture in vitro
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5594060/
https://www.ncbi.nlm.nih.gov/pubmed/28936163
http://dx.doi.org/10.3389/fnmol.2017.00285
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