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RNA flow cytometric FISH for investigations into HIV immunology, vaccination and cure strategies
Despite the tremendous success of anti-retroviral therapy (ART) no current treatment can eradicate latent HIV reservoirs from HIV-infected individuals or generate, effective HIV-specific immunity. Technological limitations have hampered the identification and characterization of both HIV-infected ce...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5594475/ https://www.ncbi.nlm.nih.gov/pubmed/28893281 http://dx.doi.org/10.1186/s12981-017-0171-x |
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author | Baxter, Amy E. Niessl, Julia Morou, Antigoni Kaufmann, Daniel E. |
author_facet | Baxter, Amy E. Niessl, Julia Morou, Antigoni Kaufmann, Daniel E. |
author_sort | Baxter, Amy E. |
collection | PubMed |
description | Despite the tremendous success of anti-retroviral therapy (ART) no current treatment can eradicate latent HIV reservoirs from HIV-infected individuals or generate, effective HIV-specific immunity. Technological limitations have hampered the identification and characterization of both HIV-infected cells and HIV-specific responses in clinical samples directly ex vivo. RNA-flow cytometric fluorescence in situ hybridisation (RNA Flow-FISH) is a powerful technique, which enables detection of mRNAs in conjunction with proteins at a single-cell level. Here, we describe how we are using this technology to address some of the major questions remaining in the HIV field in the era of ART. We discuss how CD4 T cell responses to HIV antigens, both following vaccination and HIV infection, can be characterized by measurement of cytokine mRNAs. We describe how our development of a dual HIV mRNA/protein assay (HIV(RNA/Gag) assay) enables high sensitivity detection of very rare HIV-infected cells and aids investigations into the translation-competent latent reservoir in the context of HIV cure. |
format | Online Article Text |
id | pubmed-5594475 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-55944752017-09-14 RNA flow cytometric FISH for investigations into HIV immunology, vaccination and cure strategies Baxter, Amy E. Niessl, Julia Morou, Antigoni Kaufmann, Daniel E. AIDS Res Ther Review Despite the tremendous success of anti-retroviral therapy (ART) no current treatment can eradicate latent HIV reservoirs from HIV-infected individuals or generate, effective HIV-specific immunity. Technological limitations have hampered the identification and characterization of both HIV-infected cells and HIV-specific responses in clinical samples directly ex vivo. RNA-flow cytometric fluorescence in situ hybridisation (RNA Flow-FISH) is a powerful technique, which enables detection of mRNAs in conjunction with proteins at a single-cell level. Here, we describe how we are using this technology to address some of the major questions remaining in the HIV field in the era of ART. We discuss how CD4 T cell responses to HIV antigens, both following vaccination and HIV infection, can be characterized by measurement of cytokine mRNAs. We describe how our development of a dual HIV mRNA/protein assay (HIV(RNA/Gag) assay) enables high sensitivity detection of very rare HIV-infected cells and aids investigations into the translation-competent latent reservoir in the context of HIV cure. BioMed Central 2017-09-12 /pmc/articles/PMC5594475/ /pubmed/28893281 http://dx.doi.org/10.1186/s12981-017-0171-x Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Review Baxter, Amy E. Niessl, Julia Morou, Antigoni Kaufmann, Daniel E. RNA flow cytometric FISH for investigations into HIV immunology, vaccination and cure strategies |
title | RNA flow cytometric FISH for investigations into HIV immunology, vaccination and cure strategies |
title_full | RNA flow cytometric FISH for investigations into HIV immunology, vaccination and cure strategies |
title_fullStr | RNA flow cytometric FISH for investigations into HIV immunology, vaccination and cure strategies |
title_full_unstemmed | RNA flow cytometric FISH for investigations into HIV immunology, vaccination and cure strategies |
title_short | RNA flow cytometric FISH for investigations into HIV immunology, vaccination and cure strategies |
title_sort | rna flow cytometric fish for investigations into hiv immunology, vaccination and cure strategies |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5594475/ https://www.ncbi.nlm.nih.gov/pubmed/28893281 http://dx.doi.org/10.1186/s12981-017-0171-x |
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