Cargando…

Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus

Zika virus (ZIKV) is a member of the flavivirus genus, and its genome is approximately 10.8 kilobases of positive-strand RNA enclosed in a capsid and surrounded by a membrane. Studies on the replication dynamics of ZIKV are scarce, which limits the development of antiviral agents and vaccines direct...

Descripción completa

Detalles Bibliográficos
Autores principales: Barreto-Vieira, Debora Ferreira, Jácome, Fernanda Cunha, da Silva, Marcos Alexandre Nunes, Caldas, Gabriela Cardoso, de Filippis, Ana Maria Bispo, de Sequeira, Patrícia Carvalho, de Souza, Elen Mello, Andrade, Audrien Alves, Manso, Pedro Paulo de Abreu, Trindade, Gisela Freitas, Lima, Sheila Maria Barbosa, Barth, Ortrud Monika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5595330/
https://www.ncbi.nlm.nih.gov/pubmed/28898286
http://dx.doi.org/10.1371/journal.pone.0184397
_version_ 1783263353545687040
author Barreto-Vieira, Debora Ferreira
Jácome, Fernanda Cunha
da Silva, Marcos Alexandre Nunes
Caldas, Gabriela Cardoso
de Filippis, Ana Maria Bispo
de Sequeira, Patrícia Carvalho
de Souza, Elen Mello
Andrade, Audrien Alves
Manso, Pedro Paulo de Abreu
Trindade, Gisela Freitas
Lima, Sheila Maria Barbosa
Barth, Ortrud Monika
author_facet Barreto-Vieira, Debora Ferreira
Jácome, Fernanda Cunha
da Silva, Marcos Alexandre Nunes
Caldas, Gabriela Cardoso
de Filippis, Ana Maria Bispo
de Sequeira, Patrícia Carvalho
de Souza, Elen Mello
Andrade, Audrien Alves
Manso, Pedro Paulo de Abreu
Trindade, Gisela Freitas
Lima, Sheila Maria Barbosa
Barth, Ortrud Monika
author_sort Barreto-Vieira, Debora Ferreira
collection PubMed
description Zika virus (ZIKV) is a member of the flavivirus genus, and its genome is approximately 10.8 kilobases of positive-strand RNA enclosed in a capsid and surrounded by a membrane. Studies on the replication dynamics of ZIKV are scarce, which limits the development of antiviral agents and vaccines directed against ZIKV. In this study, Aedes albopictus mosquito lineage cells (C6/36 cells) and African green monkey kidney epithelial cells (Vero cells) were inoculated with a ZIKV sample isolated from a Brazilian patient, and the infection was characterized by immunofluorescence staining, phase contrast light microscopy, transmission electron microscopy and real-time RT-PCR. The infection was observed in both cell lineages, and ZIKV particles were observed inside lysosomes, the rough endoplasmic reticulum and viroplasm-like structures. The susceptibility of C6/36 and Vero cells to ZIKV infection was demonstrated. Moreover, this study showed that part of the replicative cycle may occur within viroplasm-like structures, which has not been previously demonstrated in other flaviviruses.
format Online
Article
Text
id pubmed-5595330
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-55953302017-09-15 Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus Barreto-Vieira, Debora Ferreira Jácome, Fernanda Cunha da Silva, Marcos Alexandre Nunes Caldas, Gabriela Cardoso de Filippis, Ana Maria Bispo de Sequeira, Patrícia Carvalho de Souza, Elen Mello Andrade, Audrien Alves Manso, Pedro Paulo de Abreu Trindade, Gisela Freitas Lima, Sheila Maria Barbosa Barth, Ortrud Monika PLoS One Research Article Zika virus (ZIKV) is a member of the flavivirus genus, and its genome is approximately 10.8 kilobases of positive-strand RNA enclosed in a capsid and surrounded by a membrane. Studies on the replication dynamics of ZIKV are scarce, which limits the development of antiviral agents and vaccines directed against ZIKV. In this study, Aedes albopictus mosquito lineage cells (C6/36 cells) and African green monkey kidney epithelial cells (Vero cells) were inoculated with a ZIKV sample isolated from a Brazilian patient, and the infection was characterized by immunofluorescence staining, phase contrast light microscopy, transmission electron microscopy and real-time RT-PCR. The infection was observed in both cell lineages, and ZIKV particles were observed inside lysosomes, the rough endoplasmic reticulum and viroplasm-like structures. The susceptibility of C6/36 and Vero cells to ZIKV infection was demonstrated. Moreover, this study showed that part of the replicative cycle may occur within viroplasm-like structures, which has not been previously demonstrated in other flaviviruses. Public Library of Science 2017-09-12 /pmc/articles/PMC5595330/ /pubmed/28898286 http://dx.doi.org/10.1371/journal.pone.0184397 Text en © 2017 Barreto-Vieira et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Barreto-Vieira, Debora Ferreira
Jácome, Fernanda Cunha
da Silva, Marcos Alexandre Nunes
Caldas, Gabriela Cardoso
de Filippis, Ana Maria Bispo
de Sequeira, Patrícia Carvalho
de Souza, Elen Mello
Andrade, Audrien Alves
Manso, Pedro Paulo de Abreu
Trindade, Gisela Freitas
Lima, Sheila Maria Barbosa
Barth, Ortrud Monika
Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus
title Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus
title_full Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus
title_fullStr Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus
title_full_unstemmed Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus
title_short Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus
title_sort structural investigation of c6/36 and vero cell cultures infected with a brazilian zika virus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5595330/
https://www.ncbi.nlm.nih.gov/pubmed/28898286
http://dx.doi.org/10.1371/journal.pone.0184397
work_keys_str_mv AT barretovieiradeboraferreira structuralinvestigationofc636andverocellculturesinfectedwithabrazilianzikavirus
AT jacomefernandacunha structuralinvestigationofc636andverocellculturesinfectedwithabrazilianzikavirus
AT dasilvamarcosalexandrenunes structuralinvestigationofc636andverocellculturesinfectedwithabrazilianzikavirus
AT caldasgabrielacardoso structuralinvestigationofc636andverocellculturesinfectedwithabrazilianzikavirus
AT defilippisanamariabispo structuralinvestigationofc636andverocellculturesinfectedwithabrazilianzikavirus
AT desequeirapatriciacarvalho structuralinvestigationofc636andverocellculturesinfectedwithabrazilianzikavirus
AT desouzaelenmello structuralinvestigationofc636andverocellculturesinfectedwithabrazilianzikavirus
AT andradeaudrienalves structuralinvestigationofc636andverocellculturesinfectedwithabrazilianzikavirus
AT mansopedropaulodeabreu structuralinvestigationofc636andverocellculturesinfectedwithabrazilianzikavirus
AT trindadegiselafreitas structuralinvestigationofc636andverocellculturesinfectedwithabrazilianzikavirus
AT limasheilamariabarbosa structuralinvestigationofc636andverocellculturesinfectedwithabrazilianzikavirus
AT barthortrudmonika structuralinvestigationofc636andverocellculturesinfectedwithabrazilianzikavirus