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Enhanced synaptotagmin plasticity derived from pairing intrinsic disorder with synaptic vesicle lipids

Synaptotagmin 1 (Syt 1) is an integral membrane protein responsible for sensing the calcium ion (Ca(2+)) influx in neurons that triggers synaptic vesicle exocytosis. How Syt 1's intrinsically disordered region (IDR), a ∼60 residue sequence located between the protein's transmembrane helix...

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Autores principales: Fealey, Michael E., Thomas, David D., Hinderliter, Anne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5595411/
http://dx.doi.org/10.1080/19420889.2017.1343772
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author Fealey, Michael E.
Thomas, David D.
Hinderliter, Anne
author_facet Fealey, Michael E.
Thomas, David D.
Hinderliter, Anne
author_sort Fealey, Michael E.
collection PubMed
description Synaptotagmin 1 (Syt 1) is an integral membrane protein responsible for sensing the calcium ion (Ca(2+)) influx in neurons that triggers synaptic vesicle exocytosis. How Syt 1's intrinsically disordered region (IDR), a ∼60 residue sequence located between the protein's transmembrane helix and two Ca(2+)-sensing C2 domains, contributes to protein function is not well understood. The same is true of analogous IDRs located in the other synaptotagmin isoforms. Recently, we found that the Syt 1 IDR is structurally responsive to vesicles whose lipid composition mimics that of a synaptic vesicle organelle and that this sensitivity allosterically influences binding and folding behavior of the adjacent C2 domain. We believe these observations may be applicable to the study of other synaptotagmin isoforms and discuss generally how an IDR-membrane interaction could contribute to modulation of C2 domain function.
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spelling pubmed-55954112017-09-15 Enhanced synaptotagmin plasticity derived from pairing intrinsic disorder with synaptic vesicle lipids Fealey, Michael E. Thomas, David D. Hinderliter, Anne Commun Integr Biol Article Addendum Synaptotagmin 1 (Syt 1) is an integral membrane protein responsible for sensing the calcium ion (Ca(2+)) influx in neurons that triggers synaptic vesicle exocytosis. How Syt 1's intrinsically disordered region (IDR), a ∼60 residue sequence located between the protein's transmembrane helix and two Ca(2+)-sensing C2 domains, contributes to protein function is not well understood. The same is true of analogous IDRs located in the other synaptotagmin isoforms. Recently, we found that the Syt 1 IDR is structurally responsive to vesicles whose lipid composition mimics that of a synaptic vesicle organelle and that this sensitivity allosterically influences binding and folding behavior of the adjacent C2 domain. We believe these observations may be applicable to the study of other synaptotagmin isoforms and discuss generally how an IDR-membrane interaction could contribute to modulation of C2 domain function. Taylor & Francis 2017-07-26 /pmc/articles/PMC5595411/ http://dx.doi.org/10.1080/19420889.2017.1343772 Text en © 2017 The Author(s). Published with license by Taylor & Francis http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
spellingShingle Article Addendum
Fealey, Michael E.
Thomas, David D.
Hinderliter, Anne
Enhanced synaptotagmin plasticity derived from pairing intrinsic disorder with synaptic vesicle lipids
title Enhanced synaptotagmin plasticity derived from pairing intrinsic disorder with synaptic vesicle lipids
title_full Enhanced synaptotagmin plasticity derived from pairing intrinsic disorder with synaptic vesicle lipids
title_fullStr Enhanced synaptotagmin plasticity derived from pairing intrinsic disorder with synaptic vesicle lipids
title_full_unstemmed Enhanced synaptotagmin plasticity derived from pairing intrinsic disorder with synaptic vesicle lipids
title_short Enhanced synaptotagmin plasticity derived from pairing intrinsic disorder with synaptic vesicle lipids
title_sort enhanced synaptotagmin plasticity derived from pairing intrinsic disorder with synaptic vesicle lipids
topic Article Addendum
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5595411/
http://dx.doi.org/10.1080/19420889.2017.1343772
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