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Photobleaching in STED nanoscopy and its dependence on the photon flux applied for reversible silencing of the fluorophore
In STED (stimulated emission depletion) nanoscopy, the resolution and signal are limited by the fluorophore de-excitation efficiency and photobleaching. Here, we investigated their dependence on the pulse duration and power of the applied STED light for the popular 750 nm wavelength. In experiments...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5595794/ https://www.ncbi.nlm.nih.gov/pubmed/28900102 http://dx.doi.org/10.1038/s41598-017-09902-x |