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An automated workflow for quantifying RNA transcripts in individual cells in large data-sets

Advanced molecular probing techniques such as single molecule fluorescence in situ hybridization (smFISH) or RNAscope can be used to assess the quantity and spatial location of mRNA transcripts within cells. Quantifying mRNA expression in large image sets usually involves automated counting of fluor...

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Detalles Bibliográficos
Autores principales:	Pharris, Matthew C., Wu, Tzu-Ching, Chen, Xinping, Wang, Xu, Umulis, David M., Weake, Vikki M., Kinzer-Ursem, Tamara L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2017
Materias:	Biochemistry, Genetics and Molecular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5596354/ https://www.ncbi.nlm.nih.gov/pubmed/28932696 http://dx.doi.org/10.1016/j.mex.2017.08.002

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