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Di (2-ethylhexyl) phthalate exposure impairs meiotic progression and DNA damage repair in fetal mouse oocytes in vitro

Di (2-ethylhexyl) phthalate (DEHP), is the most common member of the class of phthalates that are used as plasticizers and have become common environmental contaminants. A number of studies have shown that DEHP exposure impacts reproductive health in both male and female mammals by acting as an estr...

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Autores principales: Liu, Jing-Cai, Lai, Fang-Nong, Li, Ling, Sun, Xiao-Feng, Cheng, Shun-Feng, Ge, Wei, Wang, Yu-Feng, Li, Lan, Zhang, Xi-Feng, De Felici, Massimo, Dyce, Paul W, Shen, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5596541/
https://www.ncbi.nlm.nih.gov/pubmed/28771232
http://dx.doi.org/10.1038/cddis.2017.350
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author Liu, Jing-Cai
Lai, Fang-Nong
Li, Ling
Sun, Xiao-Feng
Cheng, Shun-Feng
Ge, Wei
Wang, Yu-Feng
Li, Lan
Zhang, Xi-Feng
De Felici, Massimo
Dyce, Paul W
Shen, Wei
author_facet Liu, Jing-Cai
Lai, Fang-Nong
Li, Ling
Sun, Xiao-Feng
Cheng, Shun-Feng
Ge, Wei
Wang, Yu-Feng
Li, Lan
Zhang, Xi-Feng
De Felici, Massimo
Dyce, Paul W
Shen, Wei
author_sort Liu, Jing-Cai
collection PubMed
description Di (2-ethylhexyl) phthalate (DEHP), is the most common member of the class of phthalates that are used as plasticizers and have become common environmental contaminants. A number of studies have shown that DEHP exposure impacts reproductive health in both male and female mammals by acting as an estrogen analog. Here, we investigated the effects of DEHP on meiotic progression of fetal mouse oocytes by using an in vitro model of ovarian tissue culture. The results showed that 10 or 100 μM DEHP exposure inhibited the progression of oocytes throughout meiotic prophase I, specifically from the pachytene to diplotene stages. DEHP possibly impairs the ability to repair DNA double-strand breaks induced by meiotic recombination and as a consequence activates a pachytene check point. At later stages, such defects led to an increased number of oocytes showing apoptotic markers (TUNEL staining, expression of pro-apoptotic genes), resulting in reduced oocyte survival, gap junctions, and follicle assembly in the ovarian tissues. Microarray analysis of ovarian tissues exposed to DEHP showed altered expression of several genes including some involved in apoptosis and gonad development. The expression changes of some genes clustered in cell-cell communication and signal transduction, along with plasma membrane, extracellular matrix and ion channel function classes, were dependent on the DEHP concentration. Together, these results bring new support to the notion that exposure to DEHP during gestation might exert deleterious effects on ovary development, perturbing germ cell meiosis and the expression of genes involved in a wide range of biological processes including ovary development.
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spelling pubmed-55965412017-09-14 Di (2-ethylhexyl) phthalate exposure impairs meiotic progression and DNA damage repair in fetal mouse oocytes in vitro Liu, Jing-Cai Lai, Fang-Nong Li, Ling Sun, Xiao-Feng Cheng, Shun-Feng Ge, Wei Wang, Yu-Feng Li, Lan Zhang, Xi-Feng De Felici, Massimo Dyce, Paul W Shen, Wei Cell Death Dis Original Article Di (2-ethylhexyl) phthalate (DEHP), is the most common member of the class of phthalates that are used as plasticizers and have become common environmental contaminants. A number of studies have shown that DEHP exposure impacts reproductive health in both male and female mammals by acting as an estrogen analog. Here, we investigated the effects of DEHP on meiotic progression of fetal mouse oocytes by using an in vitro model of ovarian tissue culture. The results showed that 10 or 100 μM DEHP exposure inhibited the progression of oocytes throughout meiotic prophase I, specifically from the pachytene to diplotene stages. DEHP possibly impairs the ability to repair DNA double-strand breaks induced by meiotic recombination and as a consequence activates a pachytene check point. At later stages, such defects led to an increased number of oocytes showing apoptotic markers (TUNEL staining, expression of pro-apoptotic genes), resulting in reduced oocyte survival, gap junctions, and follicle assembly in the ovarian tissues. Microarray analysis of ovarian tissues exposed to DEHP showed altered expression of several genes including some involved in apoptosis and gonad development. The expression changes of some genes clustered in cell-cell communication and signal transduction, along with plasma membrane, extracellular matrix and ion channel function classes, were dependent on the DEHP concentration. Together, these results bring new support to the notion that exposure to DEHP during gestation might exert deleterious effects on ovary development, perturbing germ cell meiosis and the expression of genes involved in a wide range of biological processes including ovary development. Nature Publishing Group 2017-08 2017-08-03 /pmc/articles/PMC5596541/ /pubmed/28771232 http://dx.doi.org/10.1038/cddis.2017.350 Text en Copyright © 2017 The Author(s) http://creativecommons.org/licenses/by/4.0/ Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Original Article
Liu, Jing-Cai
Lai, Fang-Nong
Li, Ling
Sun, Xiao-Feng
Cheng, Shun-Feng
Ge, Wei
Wang, Yu-Feng
Li, Lan
Zhang, Xi-Feng
De Felici, Massimo
Dyce, Paul W
Shen, Wei
Di (2-ethylhexyl) phthalate exposure impairs meiotic progression and DNA damage repair in fetal mouse oocytes in vitro
title Di (2-ethylhexyl) phthalate exposure impairs meiotic progression and DNA damage repair in fetal mouse oocytes in vitro
title_full Di (2-ethylhexyl) phthalate exposure impairs meiotic progression and DNA damage repair in fetal mouse oocytes in vitro
title_fullStr Di (2-ethylhexyl) phthalate exposure impairs meiotic progression and DNA damage repair in fetal mouse oocytes in vitro
title_full_unstemmed Di (2-ethylhexyl) phthalate exposure impairs meiotic progression and DNA damage repair in fetal mouse oocytes in vitro
title_short Di (2-ethylhexyl) phthalate exposure impairs meiotic progression and DNA damage repair in fetal mouse oocytes in vitro
title_sort di (2-ethylhexyl) phthalate exposure impairs meiotic progression and dna damage repair in fetal mouse oocytes in vitro
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5596541/
https://www.ncbi.nlm.nih.gov/pubmed/28771232
http://dx.doi.org/10.1038/cddis.2017.350
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