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Fungal Communities Including Plant Pathogens in Near Surface Air Are Similar across Northwestern Europe

Information on the diversity of fungal spores in air is limited, and also the content of airborne spores of fungal plant pathogens is understudied. In the present study, a total of 152 air samples were taken from rooftops at urban settings in Slagelse, DK, Wageningen NL, and Rothamsted, UK together...

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Autores principales: Nicolaisen, Mogens, West, Jonathan S., Sapkota, Rumakanta, Canning, Gail G. M., Schoen, Cor, Justesen, Annemarie F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5596660/
https://www.ncbi.nlm.nih.gov/pubmed/28943873
http://dx.doi.org/10.3389/fmicb.2017.01729
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author Nicolaisen, Mogens
West, Jonathan S.
Sapkota, Rumakanta
Canning, Gail G. M.
Schoen, Cor
Justesen, Annemarie F.
author_facet Nicolaisen, Mogens
West, Jonathan S.
Sapkota, Rumakanta
Canning, Gail G. M.
Schoen, Cor
Justesen, Annemarie F.
author_sort Nicolaisen, Mogens
collection PubMed
description Information on the diversity of fungal spores in air is limited, and also the content of airborne spores of fungal plant pathogens is understudied. In the present study, a total of 152 air samples were taken from rooftops at urban settings in Slagelse, DK, Wageningen NL, and Rothamsted, UK together with 41 samples from above oilseed rape fields in Rothamsted. Samples were taken during 10-day periods in spring and autumn, each sample representing 1 day of sampling. The fungal content of samples was analyzed by metabarcoding of the fungal internal transcribed sequence 1 (ITS1) and by qPCR for specific fungi. The metabarcoding results demonstrated that season had significant effects on airborne fungal communities. In contrast, location did not have strong effects on the communities, even though locations were separated by up to 900 km. Also, a number of plant pathogens had strikingly similar patterns of abundance at the three locations. Rooftop samples were more diverse than samples taken above fields, probably reflecting greater mixing of air from a range of microenvironments for the rooftop sites. Pathogens that were known to be present in the crop were also found in air samples taken above the field. This paper is one of the first detailed studies of fungal composition in air with the focus on plant pathogens and shows that it is possible to detect a range of pathogens in rooftop air samplers using metabarcoding.
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spelling pubmed-55966602017-09-22 Fungal Communities Including Plant Pathogens in Near Surface Air Are Similar across Northwestern Europe Nicolaisen, Mogens West, Jonathan S. Sapkota, Rumakanta Canning, Gail G. M. Schoen, Cor Justesen, Annemarie F. Front Microbiol Microbiology Information on the diversity of fungal spores in air is limited, and also the content of airborne spores of fungal plant pathogens is understudied. In the present study, a total of 152 air samples were taken from rooftops at urban settings in Slagelse, DK, Wageningen NL, and Rothamsted, UK together with 41 samples from above oilseed rape fields in Rothamsted. Samples were taken during 10-day periods in spring and autumn, each sample representing 1 day of sampling. The fungal content of samples was analyzed by metabarcoding of the fungal internal transcribed sequence 1 (ITS1) and by qPCR for specific fungi. The metabarcoding results demonstrated that season had significant effects on airborne fungal communities. In contrast, location did not have strong effects on the communities, even though locations were separated by up to 900 km. Also, a number of plant pathogens had strikingly similar patterns of abundance at the three locations. Rooftop samples were more diverse than samples taken above fields, probably reflecting greater mixing of air from a range of microenvironments for the rooftop sites. Pathogens that were known to be present in the crop were also found in air samples taken above the field. This paper is one of the first detailed studies of fungal composition in air with the focus on plant pathogens and shows that it is possible to detect a range of pathogens in rooftop air samplers using metabarcoding. Frontiers Media S.A. 2017-09-08 /pmc/articles/PMC5596660/ /pubmed/28943873 http://dx.doi.org/10.3389/fmicb.2017.01729 Text en Copyright © 2017 Nicolaisen, West, Sapkota, Canning, Schoen and Justesen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Nicolaisen, Mogens
West, Jonathan S.
Sapkota, Rumakanta
Canning, Gail G. M.
Schoen, Cor
Justesen, Annemarie F.
Fungal Communities Including Plant Pathogens in Near Surface Air Are Similar across Northwestern Europe
title Fungal Communities Including Plant Pathogens in Near Surface Air Are Similar across Northwestern Europe
title_full Fungal Communities Including Plant Pathogens in Near Surface Air Are Similar across Northwestern Europe
title_fullStr Fungal Communities Including Plant Pathogens in Near Surface Air Are Similar across Northwestern Europe
title_full_unstemmed Fungal Communities Including Plant Pathogens in Near Surface Air Are Similar across Northwestern Europe
title_short Fungal Communities Including Plant Pathogens in Near Surface Air Are Similar across Northwestern Europe
title_sort fungal communities including plant pathogens in near surface air are similar across northwestern europe
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5596660/
https://www.ncbi.nlm.nih.gov/pubmed/28943873
http://dx.doi.org/10.3389/fmicb.2017.01729
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