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Vochysia rufa Stem Bark Extract Protects Endothelial Cells against High Glucose Damage

Background: Increased oxidative stress by persistent hyperglycemia is a widely accepted factor in vascular damage responsible for type 2 diabetes complications. The plant Vochysia rufa (Vr) has been used in folk medicine in Brazil for the treatment of diabetes. Thus; the protective effect of a Vr st...

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Autores principales: Moura de Gouveia, Neire, Ramos, Sonia, Martín, María Ángeles, Espindola, Foued Salmen, Goya, Luis, Palomino, Olga M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597076/
https://www.ncbi.nlm.nih.gov/pubmed/28930225
http://dx.doi.org/10.3390/medicines4010009
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author Moura de Gouveia, Neire
Ramos, Sonia
Martín, María Ángeles
Espindola, Foued Salmen
Goya, Luis
Palomino, Olga M.
author_facet Moura de Gouveia, Neire
Ramos, Sonia
Martín, María Ángeles
Espindola, Foued Salmen
Goya, Luis
Palomino, Olga M.
author_sort Moura de Gouveia, Neire
collection PubMed
description Background: Increased oxidative stress by persistent hyperglycemia is a widely accepted factor in vascular damage responsible for type 2 diabetes complications. The plant Vochysia rufa (Vr) has been used in folk medicine in Brazil for the treatment of diabetes. Thus; the protective effect of a Vr stem bark extract against a challenge by a high glucose concentration on EA.hy926 (EA) endothelial cells is evaluated. Methods: Vegetal material is extracted with distilled water by maceration and evaporated until dryness under vacuum. Then; it is isolated by capillary electrophoresis–tandem mass spectrometry. Cell viability is evaluated on EA cells treated with 0.5–100 µg/mL of the Vr extract for 24 h. The extract is diluted at concentrations of 5, 10 and 25 µg/mL and maintained for 24 h along with 30 mM of glucose to evaluate its protective effect on reduced glutathione (GSH); glutathione peroxidase (GPx) and reductase (GR) and protein carbonyl groups. Results: V. rufa stem bark is composed mainly of sugars; such as inositol; galactose; glucose; mannose; sacarose; arabinose and ribose. Treatment with Vr up to 100 µg/mL for 24 h did not affect cell viability. Treatment of EA cells with 30 mM of glucose for 24 h significantly increased the cell damage. EA cells treated with 30 mM of glucose showed a decrease of GSH concentration and increased Radical Oxygen Species (ROS) and activity of antioxidant enzymes and protein carbonyl levels; compared to control. Co-treatment of EA with 30 mM glucose plus 1–10 μg/mL Vr significantly reduced cell damage while 5–25 μg/mL Vr evoked a significant protection against the glucose insult; recovering ROS; GSH; antioxidant enzymes and carbonyls to baseline levels. Conclusion: V. rufa extract protects endothelial cells against oxidative damage by modulating ROS; GSH concentration; antioxidant enzyme activity and protein carbonyl levels.
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spelling pubmed-55970762017-09-14 Vochysia rufa Stem Bark Extract Protects Endothelial Cells against High Glucose Damage Moura de Gouveia, Neire Ramos, Sonia Martín, María Ángeles Espindola, Foued Salmen Goya, Luis Palomino, Olga M. Medicines (Basel) Article Background: Increased oxidative stress by persistent hyperglycemia is a widely accepted factor in vascular damage responsible for type 2 diabetes complications. The plant Vochysia rufa (Vr) has been used in folk medicine in Brazil for the treatment of diabetes. Thus; the protective effect of a Vr stem bark extract against a challenge by a high glucose concentration on EA.hy926 (EA) endothelial cells is evaluated. Methods: Vegetal material is extracted with distilled water by maceration and evaporated until dryness under vacuum. Then; it is isolated by capillary electrophoresis–tandem mass spectrometry. Cell viability is evaluated on EA cells treated with 0.5–100 µg/mL of the Vr extract for 24 h. The extract is diluted at concentrations of 5, 10 and 25 µg/mL and maintained for 24 h along with 30 mM of glucose to evaluate its protective effect on reduced glutathione (GSH); glutathione peroxidase (GPx) and reductase (GR) and protein carbonyl groups. Results: V. rufa stem bark is composed mainly of sugars; such as inositol; galactose; glucose; mannose; sacarose; arabinose and ribose. Treatment with Vr up to 100 µg/mL for 24 h did not affect cell viability. Treatment of EA cells with 30 mM of glucose for 24 h significantly increased the cell damage. EA cells treated with 30 mM of glucose showed a decrease of GSH concentration and increased Radical Oxygen Species (ROS) and activity of antioxidant enzymes and protein carbonyl levels; compared to control. Co-treatment of EA with 30 mM glucose plus 1–10 μg/mL Vr significantly reduced cell damage while 5–25 μg/mL Vr evoked a significant protection against the glucose insult; recovering ROS; GSH; antioxidant enzymes and carbonyls to baseline levels. Conclusion: V. rufa extract protects endothelial cells against oxidative damage by modulating ROS; GSH concentration; antioxidant enzyme activity and protein carbonyl levels. MDPI 2017-02-21 /pmc/articles/PMC5597076/ /pubmed/28930225 http://dx.doi.org/10.3390/medicines4010009 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Moura de Gouveia, Neire
Ramos, Sonia
Martín, María Ángeles
Espindola, Foued Salmen
Goya, Luis
Palomino, Olga M.
Vochysia rufa Stem Bark Extract Protects Endothelial Cells against High Glucose Damage
title Vochysia rufa Stem Bark Extract Protects Endothelial Cells against High Glucose Damage
title_full Vochysia rufa Stem Bark Extract Protects Endothelial Cells against High Glucose Damage
title_fullStr Vochysia rufa Stem Bark Extract Protects Endothelial Cells against High Glucose Damage
title_full_unstemmed Vochysia rufa Stem Bark Extract Protects Endothelial Cells against High Glucose Damage
title_short Vochysia rufa Stem Bark Extract Protects Endothelial Cells against High Glucose Damage
title_sort vochysia rufa stem bark extract protects endothelial cells against high glucose damage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597076/
https://www.ncbi.nlm.nih.gov/pubmed/28930225
http://dx.doi.org/10.3390/medicines4010009
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