Cargando…

Optimization and Characterization of Chitosan Enzymolysis by Pepsin

Pepsin was used to effectively degrade chitosan in order to make it more useful in biotechnological applications. The optimal conditions of enzymolysis were investigated on the basis of the response surface methodology (RSM). The structure of the degraded product was characterized by degree of depol...

Descripción completa

Detalles Bibliográficos
Autores principales: Gohi, Bi Foua Claude Alain, Zeng, Hong-Yan, Pan, A Dan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597186/
https://www.ncbi.nlm.nih.gov/pubmed/28952579
http://dx.doi.org/10.3390/bioengineering3030017
_version_ 1783263665426792448
author Gohi, Bi Foua Claude Alain
Zeng, Hong-Yan
Pan, A Dan
author_facet Gohi, Bi Foua Claude Alain
Zeng, Hong-Yan
Pan, A Dan
author_sort Gohi, Bi Foua Claude Alain
collection PubMed
description Pepsin was used to effectively degrade chitosan in order to make it more useful in biotechnological applications. The optimal conditions of enzymolysis were investigated on the basis of the response surface methodology (RSM). The structure of the degraded product was characterized by degree of depolymerization (DD), viscosity, molecular weight, FTIR, UV-VIS, SEM and polydispersity index analyses. The mechanism of chitosan degradation was correlated with cleavage of the glycosidic bond, whereby the chain of chitosan macromolecules was broken into smaller units, resulting in decreasing viscosity. The enzymolysis by pepsin was therefore a potentially applicable technique for the production of low molecular chitosan. Additionally, the substrate degradation kinetics of chitosan were also studied over a range of initial chitosan concentrations (3.0~18.0 g/L) in order to study the characteristics of chitosan degradation. The dependence of the rate of chitosan degradation on the concentration of the chitosan can be described by Haldane’s model. In this model, the initial chitosan concentration above which the pepsin undergoes inhibition is inferred theoretically to be about 10.5 g/L.
format Online
Article
Text
id pubmed-5597186
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-55971862017-09-21 Optimization and Characterization of Chitosan Enzymolysis by Pepsin Gohi, Bi Foua Claude Alain Zeng, Hong-Yan Pan, A Dan Bioengineering (Basel) Article Pepsin was used to effectively degrade chitosan in order to make it more useful in biotechnological applications. The optimal conditions of enzymolysis were investigated on the basis of the response surface methodology (RSM). The structure of the degraded product was characterized by degree of depolymerization (DD), viscosity, molecular weight, FTIR, UV-VIS, SEM and polydispersity index analyses. The mechanism of chitosan degradation was correlated with cleavage of the glycosidic bond, whereby the chain of chitosan macromolecules was broken into smaller units, resulting in decreasing viscosity. The enzymolysis by pepsin was therefore a potentially applicable technique for the production of low molecular chitosan. Additionally, the substrate degradation kinetics of chitosan were also studied over a range of initial chitosan concentrations (3.0~18.0 g/L) in order to study the characteristics of chitosan degradation. The dependence of the rate of chitosan degradation on the concentration of the chitosan can be described by Haldane’s model. In this model, the initial chitosan concentration above which the pepsin undergoes inhibition is inferred theoretically to be about 10.5 g/L. MDPI 2016-07-01 /pmc/articles/PMC5597186/ /pubmed/28952579 http://dx.doi.org/10.3390/bioengineering3030017 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gohi, Bi Foua Claude Alain
Zeng, Hong-Yan
Pan, A Dan
Optimization and Characterization of Chitosan Enzymolysis by Pepsin
title Optimization and Characterization of Chitosan Enzymolysis by Pepsin
title_full Optimization and Characterization of Chitosan Enzymolysis by Pepsin
title_fullStr Optimization and Characterization of Chitosan Enzymolysis by Pepsin
title_full_unstemmed Optimization and Characterization of Chitosan Enzymolysis by Pepsin
title_short Optimization and Characterization of Chitosan Enzymolysis by Pepsin
title_sort optimization and characterization of chitosan enzymolysis by pepsin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597186/
https://www.ncbi.nlm.nih.gov/pubmed/28952579
http://dx.doi.org/10.3390/bioengineering3030017
work_keys_str_mv AT gohibifouaclaudealain optimizationandcharacterizationofchitosanenzymolysisbypepsin
AT zenghongyan optimizationandcharacterizationofchitosanenzymolysisbypepsin
AT panadan optimizationandcharacterizationofchitosanenzymolysisbypepsin