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Optimization and Characterization of Chitosan Enzymolysis by Pepsin
Pepsin was used to effectively degrade chitosan in order to make it more useful in biotechnological applications. The optimal conditions of enzymolysis were investigated on the basis of the response surface methodology (RSM). The structure of the degraded product was characterized by degree of depol...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597186/ https://www.ncbi.nlm.nih.gov/pubmed/28952579 http://dx.doi.org/10.3390/bioengineering3030017 |
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author | Gohi, Bi Foua Claude Alain Zeng, Hong-Yan Pan, A Dan |
author_facet | Gohi, Bi Foua Claude Alain Zeng, Hong-Yan Pan, A Dan |
author_sort | Gohi, Bi Foua Claude Alain |
collection | PubMed |
description | Pepsin was used to effectively degrade chitosan in order to make it more useful in biotechnological applications. The optimal conditions of enzymolysis were investigated on the basis of the response surface methodology (RSM). The structure of the degraded product was characterized by degree of depolymerization (DD), viscosity, molecular weight, FTIR, UV-VIS, SEM and polydispersity index analyses. The mechanism of chitosan degradation was correlated with cleavage of the glycosidic bond, whereby the chain of chitosan macromolecules was broken into smaller units, resulting in decreasing viscosity. The enzymolysis by pepsin was therefore a potentially applicable technique for the production of low molecular chitosan. Additionally, the substrate degradation kinetics of chitosan were also studied over a range of initial chitosan concentrations (3.0~18.0 g/L) in order to study the characteristics of chitosan degradation. The dependence of the rate of chitosan degradation on the concentration of the chitosan can be described by Haldane’s model. In this model, the initial chitosan concentration above which the pepsin undergoes inhibition is inferred theoretically to be about 10.5 g/L. |
format | Online Article Text |
id | pubmed-5597186 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-55971862017-09-21 Optimization and Characterization of Chitosan Enzymolysis by Pepsin Gohi, Bi Foua Claude Alain Zeng, Hong-Yan Pan, A Dan Bioengineering (Basel) Article Pepsin was used to effectively degrade chitosan in order to make it more useful in biotechnological applications. The optimal conditions of enzymolysis were investigated on the basis of the response surface methodology (RSM). The structure of the degraded product was characterized by degree of depolymerization (DD), viscosity, molecular weight, FTIR, UV-VIS, SEM and polydispersity index analyses. The mechanism of chitosan degradation was correlated with cleavage of the glycosidic bond, whereby the chain of chitosan macromolecules was broken into smaller units, resulting in decreasing viscosity. The enzymolysis by pepsin was therefore a potentially applicable technique for the production of low molecular chitosan. Additionally, the substrate degradation kinetics of chitosan were also studied over a range of initial chitosan concentrations (3.0~18.0 g/L) in order to study the characteristics of chitosan degradation. The dependence of the rate of chitosan degradation on the concentration of the chitosan can be described by Haldane’s model. In this model, the initial chitosan concentration above which the pepsin undergoes inhibition is inferred theoretically to be about 10.5 g/L. MDPI 2016-07-01 /pmc/articles/PMC5597186/ /pubmed/28952579 http://dx.doi.org/10.3390/bioengineering3030017 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gohi, Bi Foua Claude Alain Zeng, Hong-Yan Pan, A Dan Optimization and Characterization of Chitosan Enzymolysis by Pepsin |
title | Optimization and Characterization of Chitosan Enzymolysis by Pepsin |
title_full | Optimization and Characterization of Chitosan Enzymolysis by Pepsin |
title_fullStr | Optimization and Characterization of Chitosan Enzymolysis by Pepsin |
title_full_unstemmed | Optimization and Characterization of Chitosan Enzymolysis by Pepsin |
title_short | Optimization and Characterization of Chitosan Enzymolysis by Pepsin |
title_sort | optimization and characterization of chitosan enzymolysis by pepsin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597186/ https://www.ncbi.nlm.nih.gov/pubmed/28952579 http://dx.doi.org/10.3390/bioengineering3030017 |
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