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Optimization of the expression, purification and polymerase activity reaction conditions of recombinant human PrimPol

Human PrimPol is a DNA primase/polymerase involved in DNA damage tolerance and prevents nuclear genome instability. PrimPol is also localized to the mitochondria, but its precise function in mitochondrial DNA maintenance has remained elusive. PrimPol works both as a translesion (TLS) polymerase and...

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Detalles Bibliográficos
Autores principales:	Boldinova, Elizaveta O., Stojkovič, Gorazd, Khairullin, Rafil, Wanrooij, Sjoerd, Makarova, Alena V.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2017
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597260/ https://www.ncbi.nlm.nih.gov/pubmed/28902865 http://dx.doi.org/10.1371/journal.pone.0184489

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