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A Simple Picaxe Microcontroller Pulse Source for Juxtacellular Neuronal Labelling †

Juxtacellular neuronal labelling is a method which allows neurophysiologists to fill physiologically-identified neurons with small positively-charged marker molecules. Labelled neurons are identified by histochemical processing of brain sections along with immunohistochemical identification of neuro...

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Autor principal: Verberne, Anthony J. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597270/
https://www.ncbi.nlm.nih.gov/pubmed/28952589
http://dx.doi.org/10.3390/bioengineering3040027
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author Verberne, Anthony J. M.
author_facet Verberne, Anthony J. M.
author_sort Verberne, Anthony J. M.
collection PubMed
description Juxtacellular neuronal labelling is a method which allows neurophysiologists to fill physiologically-identified neurons with small positively-charged marker molecules. Labelled neurons are identified by histochemical processing of brain sections along with immunohistochemical identification of neuropeptides, neurotransmitters, neurotransmitter transporters or biosynthetic enzymes. A microcontroller-based pulser circuit and associated BASIC software script is described for incorporation into the design of a commercially-available intracellular electrometer for use in juxtacellular neuronal labelling. Printed circuit board construction has been used for reliability and reproducibility. The current design obviates the need for a separate digital pulse source and simplifies the juxtacellular neuronal labelling procedure.
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spelling pubmed-55972702017-09-21 A Simple Picaxe Microcontroller Pulse Source for Juxtacellular Neuronal Labelling † Verberne, Anthony J. M. Bioengineering (Basel) Communication Juxtacellular neuronal labelling is a method which allows neurophysiologists to fill physiologically-identified neurons with small positively-charged marker molecules. Labelled neurons are identified by histochemical processing of brain sections along with immunohistochemical identification of neuropeptides, neurotransmitters, neurotransmitter transporters or biosynthetic enzymes. A microcontroller-based pulser circuit and associated BASIC software script is described for incorporation into the design of a commercially-available intracellular electrometer for use in juxtacellular neuronal labelling. Printed circuit board construction has been used for reliability and reproducibility. The current design obviates the need for a separate digital pulse source and simplifies the juxtacellular neuronal labelling procedure. MDPI 2016-10-19 /pmc/articles/PMC5597270/ /pubmed/28952589 http://dx.doi.org/10.3390/bioengineering3040027 Text en © 2016 by the author. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Verberne, Anthony J. M.
A Simple Picaxe Microcontroller Pulse Source for Juxtacellular Neuronal Labelling †
title A Simple Picaxe Microcontroller Pulse Source for Juxtacellular Neuronal Labelling †
title_full A Simple Picaxe Microcontroller Pulse Source for Juxtacellular Neuronal Labelling †
title_fullStr A Simple Picaxe Microcontroller Pulse Source for Juxtacellular Neuronal Labelling †
title_full_unstemmed A Simple Picaxe Microcontroller Pulse Source for Juxtacellular Neuronal Labelling †
title_short A Simple Picaxe Microcontroller Pulse Source for Juxtacellular Neuronal Labelling †
title_sort simple picaxe microcontroller pulse source for juxtacellular neuronal labelling †
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597270/
https://www.ncbi.nlm.nih.gov/pubmed/28952589
http://dx.doi.org/10.3390/bioengineering3040027
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