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Longitudinal Stretching for Maturation of Vascular Tissues Using Magnetic Forces
Cellular spheroids were studied to determine their use as “bioinks” in the biofabrication of tissue engineered constructs. Specifically, magnetic forces were used to mediate the cyclic longitudinal stretching of tissues composed of Janus magnetic cellular spheroids (JMCSs), as part of a post-process...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597272/ https://www.ncbi.nlm.nih.gov/pubmed/28952591 http://dx.doi.org/10.3390/bioengineering3040029 |
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author | Olsen, Timothy R. Casco, Megan Herbst, Austin Evans, Grace Rothermel, Taylor Pruett, Lauren Reid, Jared Barry, Kelly Jaeggli, Michael P. Simionescu, Dan T. Visconti, Richard P. Alexis, Frank |
author_facet | Olsen, Timothy R. Casco, Megan Herbst, Austin Evans, Grace Rothermel, Taylor Pruett, Lauren Reid, Jared Barry, Kelly Jaeggli, Michael P. Simionescu, Dan T. Visconti, Richard P. Alexis, Frank |
author_sort | Olsen, Timothy R. |
collection | PubMed |
description | Cellular spheroids were studied to determine their use as “bioinks” in the biofabrication of tissue engineered constructs. Specifically, magnetic forces were used to mediate the cyclic longitudinal stretching of tissues composed of Janus magnetic cellular spheroids (JMCSs), as part of a post-processing method for enhancing the deposition and mechanical properties of an extracellular matrix (ECM). The purpose was to accelerate the conventional tissue maturation process via novel post-processing techniques that accelerate the functional, structural, and mechanical mimicking of native tissues. The results of a forty-day study of JMCSs indicated an expression of collagen I, collagen IV, elastin, and fibronectin, which are important vascular ECM proteins. Most notably, the subsequent exposure of fused tissue sheets composed of JMCSs to magnetic forces did not hinder the production of these key proteins. Quantitative results demonstrate that cyclic longitudinal stretching of the tissue sheets mediated by these magnetic forces increased the Young’s modulus and induced collagen fiber alignment over a seven day period, when compared to statically conditioned controls. Specifically, the elastin and collagen content of these dynamically-conditioned sheets were 35- and three-fold greater, respectively, at seven days compared to the statically-conditioned controls at three days. These findings indicate the potential of using magnetic forces in tissue maturation, specifically through the cyclic longitudinal stretching of tissues. |
format | Online Article Text |
id | pubmed-5597272 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-55972722017-09-21 Longitudinal Stretching for Maturation of Vascular Tissues Using Magnetic Forces Olsen, Timothy R. Casco, Megan Herbst, Austin Evans, Grace Rothermel, Taylor Pruett, Lauren Reid, Jared Barry, Kelly Jaeggli, Michael P. Simionescu, Dan T. Visconti, Richard P. Alexis, Frank Bioengineering (Basel) Article Cellular spheroids were studied to determine their use as “bioinks” in the biofabrication of tissue engineered constructs. Specifically, magnetic forces were used to mediate the cyclic longitudinal stretching of tissues composed of Janus magnetic cellular spheroids (JMCSs), as part of a post-processing method for enhancing the deposition and mechanical properties of an extracellular matrix (ECM). The purpose was to accelerate the conventional tissue maturation process via novel post-processing techniques that accelerate the functional, structural, and mechanical mimicking of native tissues. The results of a forty-day study of JMCSs indicated an expression of collagen I, collagen IV, elastin, and fibronectin, which are important vascular ECM proteins. Most notably, the subsequent exposure of fused tissue sheets composed of JMCSs to magnetic forces did not hinder the production of these key proteins. Quantitative results demonstrate that cyclic longitudinal stretching of the tissue sheets mediated by these magnetic forces increased the Young’s modulus and induced collagen fiber alignment over a seven day period, when compared to statically conditioned controls. Specifically, the elastin and collagen content of these dynamically-conditioned sheets were 35- and three-fold greater, respectively, at seven days compared to the statically-conditioned controls at three days. These findings indicate the potential of using magnetic forces in tissue maturation, specifically through the cyclic longitudinal stretching of tissues. MDPI 2016-11-16 /pmc/articles/PMC5597272/ /pubmed/28952591 http://dx.doi.org/10.3390/bioengineering3040029 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Olsen, Timothy R. Casco, Megan Herbst, Austin Evans, Grace Rothermel, Taylor Pruett, Lauren Reid, Jared Barry, Kelly Jaeggli, Michael P. Simionescu, Dan T. Visconti, Richard P. Alexis, Frank Longitudinal Stretching for Maturation of Vascular Tissues Using Magnetic Forces |
title | Longitudinal Stretching for Maturation of Vascular Tissues Using Magnetic Forces |
title_full | Longitudinal Stretching for Maturation of Vascular Tissues Using Magnetic Forces |
title_fullStr | Longitudinal Stretching for Maturation of Vascular Tissues Using Magnetic Forces |
title_full_unstemmed | Longitudinal Stretching for Maturation of Vascular Tissues Using Magnetic Forces |
title_short | Longitudinal Stretching for Maturation of Vascular Tissues Using Magnetic Forces |
title_sort | longitudinal stretching for maturation of vascular tissues using magnetic forces |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597272/ https://www.ncbi.nlm.nih.gov/pubmed/28952591 http://dx.doi.org/10.3390/bioengineering3040029 |
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