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Longitudinal Stretching for Maturation of Vascular Tissues Using Magnetic Forces

Cellular spheroids were studied to determine their use as “bioinks” in the biofabrication of tissue engineered constructs. Specifically, magnetic forces were used to mediate the cyclic longitudinal stretching of tissues composed of Janus magnetic cellular spheroids (JMCSs), as part of a post-process...

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Autores principales: Olsen, Timothy R., Casco, Megan, Herbst, Austin, Evans, Grace, Rothermel, Taylor, Pruett, Lauren, Reid, Jared, Barry, Kelly, Jaeggli, Michael P., Simionescu, Dan T., Visconti, Richard P., Alexis, Frank
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597272/
https://www.ncbi.nlm.nih.gov/pubmed/28952591
http://dx.doi.org/10.3390/bioengineering3040029
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author Olsen, Timothy R.
Casco, Megan
Herbst, Austin
Evans, Grace
Rothermel, Taylor
Pruett, Lauren
Reid, Jared
Barry, Kelly
Jaeggli, Michael P.
Simionescu, Dan T.
Visconti, Richard P.
Alexis, Frank
author_facet Olsen, Timothy R.
Casco, Megan
Herbst, Austin
Evans, Grace
Rothermel, Taylor
Pruett, Lauren
Reid, Jared
Barry, Kelly
Jaeggli, Michael P.
Simionescu, Dan T.
Visconti, Richard P.
Alexis, Frank
author_sort Olsen, Timothy R.
collection PubMed
description Cellular spheroids were studied to determine their use as “bioinks” in the biofabrication of tissue engineered constructs. Specifically, magnetic forces were used to mediate the cyclic longitudinal stretching of tissues composed of Janus magnetic cellular spheroids (JMCSs), as part of a post-processing method for enhancing the deposition and mechanical properties of an extracellular matrix (ECM). The purpose was to accelerate the conventional tissue maturation process via novel post-processing techniques that accelerate the functional, structural, and mechanical mimicking of native tissues. The results of a forty-day study of JMCSs indicated an expression of collagen I, collagen IV, elastin, and fibronectin, which are important vascular ECM proteins. Most notably, the subsequent exposure of fused tissue sheets composed of JMCSs to magnetic forces did not hinder the production of these key proteins. Quantitative results demonstrate that cyclic longitudinal stretching of the tissue sheets mediated by these magnetic forces increased the Young’s modulus and induced collagen fiber alignment over a seven day period, when compared to statically conditioned controls. Specifically, the elastin and collagen content of these dynamically-conditioned sheets were 35- and three-fold greater, respectively, at seven days compared to the statically-conditioned controls at three days. These findings indicate the potential of using magnetic forces in tissue maturation, specifically through the cyclic longitudinal stretching of tissues.
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spelling pubmed-55972722017-09-21 Longitudinal Stretching for Maturation of Vascular Tissues Using Magnetic Forces Olsen, Timothy R. Casco, Megan Herbst, Austin Evans, Grace Rothermel, Taylor Pruett, Lauren Reid, Jared Barry, Kelly Jaeggli, Michael P. Simionescu, Dan T. Visconti, Richard P. Alexis, Frank Bioengineering (Basel) Article Cellular spheroids were studied to determine their use as “bioinks” in the biofabrication of tissue engineered constructs. Specifically, magnetic forces were used to mediate the cyclic longitudinal stretching of tissues composed of Janus magnetic cellular spheroids (JMCSs), as part of a post-processing method for enhancing the deposition and mechanical properties of an extracellular matrix (ECM). The purpose was to accelerate the conventional tissue maturation process via novel post-processing techniques that accelerate the functional, structural, and mechanical mimicking of native tissues. The results of a forty-day study of JMCSs indicated an expression of collagen I, collagen IV, elastin, and fibronectin, which are important vascular ECM proteins. Most notably, the subsequent exposure of fused tissue sheets composed of JMCSs to magnetic forces did not hinder the production of these key proteins. Quantitative results demonstrate that cyclic longitudinal stretching of the tissue sheets mediated by these magnetic forces increased the Young’s modulus and induced collagen fiber alignment over a seven day period, when compared to statically conditioned controls. Specifically, the elastin and collagen content of these dynamically-conditioned sheets were 35- and three-fold greater, respectively, at seven days compared to the statically-conditioned controls at three days. These findings indicate the potential of using magnetic forces in tissue maturation, specifically through the cyclic longitudinal stretching of tissues. MDPI 2016-11-16 /pmc/articles/PMC5597272/ /pubmed/28952591 http://dx.doi.org/10.3390/bioengineering3040029 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Olsen, Timothy R.
Casco, Megan
Herbst, Austin
Evans, Grace
Rothermel, Taylor
Pruett, Lauren
Reid, Jared
Barry, Kelly
Jaeggli, Michael P.
Simionescu, Dan T.
Visconti, Richard P.
Alexis, Frank
Longitudinal Stretching for Maturation of Vascular Tissues Using Magnetic Forces
title Longitudinal Stretching for Maturation of Vascular Tissues Using Magnetic Forces
title_full Longitudinal Stretching for Maturation of Vascular Tissues Using Magnetic Forces
title_fullStr Longitudinal Stretching for Maturation of Vascular Tissues Using Magnetic Forces
title_full_unstemmed Longitudinal Stretching for Maturation of Vascular Tissues Using Magnetic Forces
title_short Longitudinal Stretching for Maturation of Vascular Tissues Using Magnetic Forces
title_sort longitudinal stretching for maturation of vascular tissues using magnetic forces
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597272/
https://www.ncbi.nlm.nih.gov/pubmed/28952591
http://dx.doi.org/10.3390/bioengineering3040029
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