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Improving heterologous membrane protein production in Escherichia coli by combining transcriptional tuning and codon usage algorithms
High-level, recombinant production of membrane-integrated proteins in Escherichia coli is extremely relevant for many purposes, but has also been proven challenging. Here we study a combination of transcriptional fine-tuning in E. coli LEMO21(DE3) with different codon usage algorithms for heterologo...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597330/ https://www.ncbi.nlm.nih.gov/pubmed/28902855 http://dx.doi.org/10.1371/journal.pone.0184355 |