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Protective effects of grape seed and skin extract against high-fat-diet-induced lipotoxicity in rat lung

BACKGROUND: Obesity is a public health problem characterized by increased fat accumulation in different tissues. Obesity is directly linked to breathing problems and medical complications with lung, including obstructive sleep apnea syndrome, obesity hypoventilation syndrome, chronic obstructive pul...

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Autores principales: El Ayed, Mohamed, Kadri, Safwen, Smine, Selima, Elkahoui, Salem, Limam, Ferid, Aouani, Ezzedine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598067/
https://www.ncbi.nlm.nih.gov/pubmed/28903761
http://dx.doi.org/10.1186/s12944-017-0561-z
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author El Ayed, Mohamed
Kadri, Safwen
Smine, Selima
Elkahoui, Salem
Limam, Ferid
Aouani, Ezzedine
author_facet El Ayed, Mohamed
Kadri, Safwen
Smine, Selima
Elkahoui, Salem
Limam, Ferid
Aouani, Ezzedine
author_sort El Ayed, Mohamed
collection PubMed
description BACKGROUND: Obesity is a public health problem characterized by increased fat accumulation in different tissues. Obesity is directly linked to breathing problems and medical complications with lung, including obstructive sleep apnea syndrome, obesity hypoventilation syndrome, chronic obstructive pulmonary disease, asthma….In the present work, we aimed to investigate the effect of high fat diet (HFD) on lung lipotoxicity, oxidative stress, fatty acid composition and proportions in lung and implication in asthma development. The likely protection provided by grape seed extract (GSSE) was also investigated. METHODS: In order to assess HFD effect on lung and GSSE protection we used a rat model. We analyzed the lipid plasma profile, lung peroxidation and antioxidant activities (SOD, CAT and POD). We also analyzed transition metals (Ca2+, Mg2+, Zn2+ and iron) and lung free fatty acids using gas chromatography coupled to mass spectrometry (GC-MS). RESULTS: HFD induced lipid profile imbalance increasing cholesterol and VLDL-C. HFD also induced an oxidative stress assessed by elevated MDA level and the drop of antioxidant activities such as SOD, CAT and POD. Moreover, HFD induced mineral disturbances by decreasing magnesium level and increasing Calcium and iron levels. HFD induced also disturbances in lung fatty acid composition by increasing oleic, stearic and arachidonic acids. Interestingly, GSSE alleviated all these deleterious effects of HFD treatment. CONCLUSION: As a whole, GSSE had a significant preventive effect against HFD-induced obesity, and hence may be used as an anti-obesity agent, and a benefic agent with potential applications against damages in lung tissue.
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spelling pubmed-55980672017-09-18 Protective effects of grape seed and skin extract against high-fat-diet-induced lipotoxicity in rat lung El Ayed, Mohamed Kadri, Safwen Smine, Selima Elkahoui, Salem Limam, Ferid Aouani, Ezzedine Lipids Health Dis Research BACKGROUND: Obesity is a public health problem characterized by increased fat accumulation in different tissues. Obesity is directly linked to breathing problems and medical complications with lung, including obstructive sleep apnea syndrome, obesity hypoventilation syndrome, chronic obstructive pulmonary disease, asthma….In the present work, we aimed to investigate the effect of high fat diet (HFD) on lung lipotoxicity, oxidative stress, fatty acid composition and proportions in lung and implication in asthma development. The likely protection provided by grape seed extract (GSSE) was also investigated. METHODS: In order to assess HFD effect on lung and GSSE protection we used a rat model. We analyzed the lipid plasma profile, lung peroxidation and antioxidant activities (SOD, CAT and POD). We also analyzed transition metals (Ca2+, Mg2+, Zn2+ and iron) and lung free fatty acids using gas chromatography coupled to mass spectrometry (GC-MS). RESULTS: HFD induced lipid profile imbalance increasing cholesterol and VLDL-C. HFD also induced an oxidative stress assessed by elevated MDA level and the drop of antioxidant activities such as SOD, CAT and POD. Moreover, HFD induced mineral disturbances by decreasing magnesium level and increasing Calcium and iron levels. HFD induced also disturbances in lung fatty acid composition by increasing oleic, stearic and arachidonic acids. Interestingly, GSSE alleviated all these deleterious effects of HFD treatment. CONCLUSION: As a whole, GSSE had a significant preventive effect against HFD-induced obesity, and hence may be used as an anti-obesity agent, and a benefic agent with potential applications against damages in lung tissue. BioMed Central 2017-09-13 /pmc/articles/PMC5598067/ /pubmed/28903761 http://dx.doi.org/10.1186/s12944-017-0561-z Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
El Ayed, Mohamed
Kadri, Safwen
Smine, Selima
Elkahoui, Salem
Limam, Ferid
Aouani, Ezzedine
Protective effects of grape seed and skin extract against high-fat-diet-induced lipotoxicity in rat lung
title Protective effects of grape seed and skin extract against high-fat-diet-induced lipotoxicity in rat lung
title_full Protective effects of grape seed and skin extract against high-fat-diet-induced lipotoxicity in rat lung
title_fullStr Protective effects of grape seed and skin extract against high-fat-diet-induced lipotoxicity in rat lung
title_full_unstemmed Protective effects of grape seed and skin extract against high-fat-diet-induced lipotoxicity in rat lung
title_short Protective effects of grape seed and skin extract against high-fat-diet-induced lipotoxicity in rat lung
title_sort protective effects of grape seed and skin extract against high-fat-diet-induced lipotoxicity in rat lung
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598067/
https://www.ncbi.nlm.nih.gov/pubmed/28903761
http://dx.doi.org/10.1186/s12944-017-0561-z
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