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RNA-seq comparative analysis of Peking ducks spleen gene expression 24 h post-infected with duck plague virulent or attenuated virus

Duck plague virus (DPV), a member of alphaherpesvirus sub-family, can cause significant economic losses on duck farms in China. DPV Chinese virulent strain (CHv) is highly pathogenic and could induce massive ducks death. Attenuated DPV vaccines (CHa) have been put into service against duck plague wi...

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Autores principales: Liu, Tian, Cheng, Anchun, Wang, Mingshu, Jia, Renyong, Yang, Qiao, Wu, Ying, Sun, Kunfeng, Zhu, Dekang, Chen, Shun, Liu, Mafeng, Zhao, XinXin, Chen, Xiaoyue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598070/
https://www.ncbi.nlm.nih.gov/pubmed/28903751
http://dx.doi.org/10.1186/s13567-017-0456-z
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author Liu, Tian
Cheng, Anchun
Wang, Mingshu
Jia, Renyong
Yang, Qiao
Wu, Ying
Sun, Kunfeng
Zhu, Dekang
Chen, Shun
Liu, Mafeng
Zhao, XinXin
Chen, Xiaoyue
author_facet Liu, Tian
Cheng, Anchun
Wang, Mingshu
Jia, Renyong
Yang, Qiao
Wu, Ying
Sun, Kunfeng
Zhu, Dekang
Chen, Shun
Liu, Mafeng
Zhao, XinXin
Chen, Xiaoyue
author_sort Liu, Tian
collection PubMed
description Duck plague virus (DPV), a member of alphaherpesvirus sub-family, can cause significant economic losses on duck farms in China. DPV Chinese virulent strain (CHv) is highly pathogenic and could induce massive ducks death. Attenuated DPV vaccines (CHa) have been put into service against duck plague with billions of doses in China each year. Researches on DPV have been development for many years, however, a comprehensive understanding of molecular mechanisms underlying pathogenicity of CHv strain and protection of CHa strain to ducks is still blank. In present study, we performed RNA-seq technology to analyze transcriptome profiling of duck spleens for the first time to identify differentially expressed genes (DEGs) associated with the infection of CHv and CHa at 24 h. Comparison of gene expression with mock ducks revealed 748 DEGs and 484 DEGs after CHv and CHa infection, respectively. Gene pathway analysis of DEGs highlighted valuable biological processes involved in host immune response, cell apoptosis and viral invasion. Genes expressed in those pathways were different in CHv infected duck spleens and CHa vaccinated duck spleens. The results may provide valuable information for us to explore the reasons of pathogenicity caused by CHv strain and protection activated by CHa strain.
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spelling pubmed-55980702017-09-18 RNA-seq comparative analysis of Peking ducks spleen gene expression 24 h post-infected with duck plague virulent or attenuated virus Liu, Tian Cheng, Anchun Wang, Mingshu Jia, Renyong Yang, Qiao Wu, Ying Sun, Kunfeng Zhu, Dekang Chen, Shun Liu, Mafeng Zhao, XinXin Chen, Xiaoyue Vet Res Research Article Duck plague virus (DPV), a member of alphaherpesvirus sub-family, can cause significant economic losses on duck farms in China. DPV Chinese virulent strain (CHv) is highly pathogenic and could induce massive ducks death. Attenuated DPV vaccines (CHa) have been put into service against duck plague with billions of doses in China each year. Researches on DPV have been development for many years, however, a comprehensive understanding of molecular mechanisms underlying pathogenicity of CHv strain and protection of CHa strain to ducks is still blank. In present study, we performed RNA-seq technology to analyze transcriptome profiling of duck spleens for the first time to identify differentially expressed genes (DEGs) associated with the infection of CHv and CHa at 24 h. Comparison of gene expression with mock ducks revealed 748 DEGs and 484 DEGs after CHv and CHa infection, respectively. Gene pathway analysis of DEGs highlighted valuable biological processes involved in host immune response, cell apoptosis and viral invasion. Genes expressed in those pathways were different in CHv infected duck spleens and CHa vaccinated duck spleens. The results may provide valuable information for us to explore the reasons of pathogenicity caused by CHv strain and protection activated by CHa strain. BioMed Central 2017-09-13 2017 /pmc/articles/PMC5598070/ /pubmed/28903751 http://dx.doi.org/10.1186/s13567-017-0456-z Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Liu, Tian
Cheng, Anchun
Wang, Mingshu
Jia, Renyong
Yang, Qiao
Wu, Ying
Sun, Kunfeng
Zhu, Dekang
Chen, Shun
Liu, Mafeng
Zhao, XinXin
Chen, Xiaoyue
RNA-seq comparative analysis of Peking ducks spleen gene expression 24 h post-infected with duck plague virulent or attenuated virus
title RNA-seq comparative analysis of Peking ducks spleen gene expression 24 h post-infected with duck plague virulent or attenuated virus
title_full RNA-seq comparative analysis of Peking ducks spleen gene expression 24 h post-infected with duck plague virulent or attenuated virus
title_fullStr RNA-seq comparative analysis of Peking ducks spleen gene expression 24 h post-infected with duck plague virulent or attenuated virus
title_full_unstemmed RNA-seq comparative analysis of Peking ducks spleen gene expression 24 h post-infected with duck plague virulent or attenuated virus
title_short RNA-seq comparative analysis of Peking ducks spleen gene expression 24 h post-infected with duck plague virulent or attenuated virus
title_sort rna-seq comparative analysis of peking ducks spleen gene expression 24 h post-infected with duck plague virulent or attenuated virus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598070/
https://www.ncbi.nlm.nih.gov/pubmed/28903751
http://dx.doi.org/10.1186/s13567-017-0456-z
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