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Cellular uptake of quercetin and luteolin and their effects on monoamine oxidase-A in human neuroblastoma SH-SY5Y cells

Monoamine oxidase-A (MAO-A) is the main enzyme in the metabolism of the neurotransmitter serotonin (5-hydroxytryptamine). Elevated activity of MAO-A in the brain may contribute to the pathogenesis of depressive disorders. Plant flavonoids, such as flavonol quercetin and flavone luteolin, have been s...

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Autores principales: Bandaruk, Yauhen, Mukai, Rie, Terao, Junji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598286/
https://www.ncbi.nlm.nih.gov/pubmed/28962277
http://dx.doi.org/10.1016/j.toxrep.2014.08.016
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author Bandaruk, Yauhen
Mukai, Rie
Terao, Junji
author_facet Bandaruk, Yauhen
Mukai, Rie
Terao, Junji
author_sort Bandaruk, Yauhen
collection PubMed
description Monoamine oxidase-A (MAO-A) is the main enzyme in the metabolism of the neurotransmitter serotonin (5-hydroxytryptamine). Elevated activity of MAO-A in the brain may contribute to the pathogenesis of depressive disorders. Plant flavonoids, such as flavonol quercetin and flavone luteolin, have been suggested to be potential antidepressant compounds because they exert a suppressive effect on the MAO-A reaction. We evaluated the effects of these flavonoids on MAO-A activity and protein level using SH-SY5Y as model serotoninergic nerve cells. Quercetin and luteolin were incorporated into SH-SY5Y cells rapidly and converted to O-methylated derivatives. Luteolin accumulated in cells after 24-h incubation, whereas quercetin disappeared completely from cell fractions and culture medium. Addition of ascorbic acid prevented the disappearance of quercetin and allowed it to exert its cytotoxicity (similar to luteolin) at >10 μM. Luteolin and quercetin were incorporated into mitochondria fractions within 1-h incubation and attenuated MAO-A activity slightly but significantly. After 24-h incubation, luteolin attenuated MAO-A activity, but quercetin needed ascorbic acid for its attenuation. Neither luteolin nor quercetin significantly affected MAO-A protein level. These data suggest that luteolin and quercetin can be direct inhibitors of MAO-A in nerve cells by targeting mitochondria.
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spelling pubmed-55982862017-09-28 Cellular uptake of quercetin and luteolin and their effects on monoamine oxidase-A in human neuroblastoma SH-SY5Y cells Bandaruk, Yauhen Mukai, Rie Terao, Junji Toxicol Rep Article Monoamine oxidase-A (MAO-A) is the main enzyme in the metabolism of the neurotransmitter serotonin (5-hydroxytryptamine). Elevated activity of MAO-A in the brain may contribute to the pathogenesis of depressive disorders. Plant flavonoids, such as flavonol quercetin and flavone luteolin, have been suggested to be potential antidepressant compounds because they exert a suppressive effect on the MAO-A reaction. We evaluated the effects of these flavonoids on MAO-A activity and protein level using SH-SY5Y as model serotoninergic nerve cells. Quercetin and luteolin were incorporated into SH-SY5Y cells rapidly and converted to O-methylated derivatives. Luteolin accumulated in cells after 24-h incubation, whereas quercetin disappeared completely from cell fractions and culture medium. Addition of ascorbic acid prevented the disappearance of quercetin and allowed it to exert its cytotoxicity (similar to luteolin) at >10 μM. Luteolin and quercetin were incorporated into mitochondria fractions within 1-h incubation and attenuated MAO-A activity slightly but significantly. After 24-h incubation, luteolin attenuated MAO-A activity, but quercetin needed ascorbic acid for its attenuation. Neither luteolin nor quercetin significantly affected MAO-A protein level. These data suggest that luteolin and quercetin can be direct inhibitors of MAO-A in nerve cells by targeting mitochondria. Elsevier 2014-09-06 /pmc/articles/PMC5598286/ /pubmed/28962277 http://dx.doi.org/10.1016/j.toxrep.2014.08.016 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Article
Bandaruk, Yauhen
Mukai, Rie
Terao, Junji
Cellular uptake of quercetin and luteolin and their effects on monoamine oxidase-A in human neuroblastoma SH-SY5Y cells
title Cellular uptake of quercetin and luteolin and their effects on monoamine oxidase-A in human neuroblastoma SH-SY5Y cells
title_full Cellular uptake of quercetin and luteolin and their effects on monoamine oxidase-A in human neuroblastoma SH-SY5Y cells
title_fullStr Cellular uptake of quercetin and luteolin and their effects on monoamine oxidase-A in human neuroblastoma SH-SY5Y cells
title_full_unstemmed Cellular uptake of quercetin and luteolin and their effects on monoamine oxidase-A in human neuroblastoma SH-SY5Y cells
title_short Cellular uptake of quercetin and luteolin and their effects on monoamine oxidase-A in human neuroblastoma SH-SY5Y cells
title_sort cellular uptake of quercetin and luteolin and their effects on monoamine oxidase-a in human neuroblastoma sh-sy5y cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598286/
https://www.ncbi.nlm.nih.gov/pubmed/28962277
http://dx.doi.org/10.1016/j.toxrep.2014.08.016
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