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Fourier transform infrared (FT-IR) study on cyanide induced biochemical and structural changes in rat sperm

In the recent years, great attention had been focused on cyanide toxicity because of its widespread use in industries and considered to be a ubiquitous pollutant in the environment. Therefore, the current study aimed to evaluate the toxic effect of cyanide on rat sperms at molecular level by using F...

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Autores principales: Shivanoor, Shiddappa Mallappa, David, Muniswamy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598370/
https://www.ncbi.nlm.nih.gov/pubmed/28962477
http://dx.doi.org/10.1016/j.toxrep.2015.10.004
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author Shivanoor, Shiddappa Mallappa
David, Muniswamy
author_facet Shivanoor, Shiddappa Mallappa
David, Muniswamy
author_sort Shivanoor, Shiddappa Mallappa
collection PubMed
description In the recent years, great attention had been focused on cyanide toxicity because of its widespread use in industries and considered to be a ubiquitous pollutant in the environment. Therefore, the current study aimed to evaluate the toxic effect of cyanide on rat sperms at molecular level by using FT-IR technique. For this purpose, rats were randomly divided into four groups and treated with 0.0, 0.64, 1.2 and 3.2 mg kg(−1) body weight (BW) for the period of 90 days. The group treated with lower dose (0.64 mg kg(−1) BW) showed an insignificant change in all the peaks, except the peaks assigned to olefinic [bond, double bond]C—H, CH(2) asymmetric and CH(2) symmetric stretching vibration in the lipids. While, the groups treated with higher doses (1.2 and 3.2 mg kg(−1) BW) showed the significant decrease in the area under the peaks corresponds to different bio-molecules. In addition, spectral second derivative analysis showed the significant alteration in α-helix, turns, β-sheet, aggregated β-sheet and random coil structures in the proteins. In conclusion, the selected higher dosage of cyanide had caused significant decrease in the biochemical composition of rat sperms along with structural changes in the proteins. The FT-IR technique is an excellent tool used for the analysis of oxidative damage in the sperms.
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spelling pubmed-55983702017-09-28 Fourier transform infrared (FT-IR) study on cyanide induced biochemical and structural changes in rat sperm Shivanoor, Shiddappa Mallappa David, Muniswamy Toxicol Rep Article In the recent years, great attention had been focused on cyanide toxicity because of its widespread use in industries and considered to be a ubiquitous pollutant in the environment. Therefore, the current study aimed to evaluate the toxic effect of cyanide on rat sperms at molecular level by using FT-IR technique. For this purpose, rats were randomly divided into four groups and treated with 0.0, 0.64, 1.2 and 3.2 mg kg(−1) body weight (BW) for the period of 90 days. The group treated with lower dose (0.64 mg kg(−1) BW) showed an insignificant change in all the peaks, except the peaks assigned to olefinic [bond, double bond]C—H, CH(2) asymmetric and CH(2) symmetric stretching vibration in the lipids. While, the groups treated with higher doses (1.2 and 3.2 mg kg(−1) BW) showed the significant decrease in the area under the peaks corresponds to different bio-molecules. In addition, spectral second derivative analysis showed the significant alteration in α-helix, turns, β-sheet, aggregated β-sheet and random coil structures in the proteins. In conclusion, the selected higher dosage of cyanide had caused significant decrease in the biochemical composition of rat sperms along with structural changes in the proteins. The FT-IR technique is an excellent tool used for the analysis of oxidative damage in the sperms. Elsevier 2015-10-22 /pmc/articles/PMC5598370/ /pubmed/28962477 http://dx.doi.org/10.1016/j.toxrep.2015.10.004 Text en © 2015 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Shivanoor, Shiddappa Mallappa
David, Muniswamy
Fourier transform infrared (FT-IR) study on cyanide induced biochemical and structural changes in rat sperm
title Fourier transform infrared (FT-IR) study on cyanide induced biochemical and structural changes in rat sperm
title_full Fourier transform infrared (FT-IR) study on cyanide induced biochemical and structural changes in rat sperm
title_fullStr Fourier transform infrared (FT-IR) study on cyanide induced biochemical and structural changes in rat sperm
title_full_unstemmed Fourier transform infrared (FT-IR) study on cyanide induced biochemical and structural changes in rat sperm
title_short Fourier transform infrared (FT-IR) study on cyanide induced biochemical and structural changes in rat sperm
title_sort fourier transform infrared (ft-ir) study on cyanide induced biochemical and structural changes in rat sperm
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598370/
https://www.ncbi.nlm.nih.gov/pubmed/28962477
http://dx.doi.org/10.1016/j.toxrep.2015.10.004
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