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Phytic acid decreases deoxynivalenol and fumonisin B1-induced changes on swine jejunal explants

The purpose of the present study was to investigate the effects of phytic acid (IP6) on morphological and immunohistochemical parameters on intestinal explants exposed to deoxynivalenol (DON) and fumonisin B1 (FB1). The jejunal explants were exposed for 4 h to different treatments: control, DON (10...

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Detalles Bibliográficos
Autores principales: da Silva, Elisângela Olegário, Gerez, Juliana Rubira, do Carmo Drape, Thalisie, Bracarense, Ana Paula F.R.L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598429/
https://www.ncbi.nlm.nih.gov/pubmed/28962245
http://dx.doi.org/10.1016/j.toxrep.2014.05.001
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author da Silva, Elisângela Olegário
Gerez, Juliana Rubira
do Carmo Drape, Thalisie
Bracarense, Ana Paula F.R.L.
author_facet da Silva, Elisângela Olegário
Gerez, Juliana Rubira
do Carmo Drape, Thalisie
Bracarense, Ana Paula F.R.L.
author_sort da Silva, Elisângela Olegário
collection PubMed
description The purpose of the present study was to investigate the effects of phytic acid (IP6) on morphological and immunohistochemical parameters on intestinal explants exposed to deoxynivalenol (DON) and fumonisin B1 (FB1). The jejunal explants were exposed for 4 h to different treatments: control, DON (10 μM), DON plus 2.5 mM or 5 mM IP6, FB1 (70 μM), and FB1 plus 2.5 mM or 5 mM IP6. Both mycotoxins induced significant intestinal lesions and decreased villi height. The presence of 2.5 mM and 5 mM IP6 significantly inhibited the morphological changes caused by the mycotoxins. DON induced a significant increase in caspase-3 (83%) and cyclooxygenase-2 (71.3%) expression compared with the control. The presence of 5 mM IP6 induced a significant decrease in caspase-3 (43.7%) and Cox-2 (48%) expression compared with the DON group. FB1 induced a significant increase in caspase-3 expression (47%) compared to the control, whereas IP6 induced no significant change in this expression. A significant decrease in cell proliferation was observed when explants were exposed to 5 mM of IP6 in comparison with the DON and FB1 groups. The present data provide evidence that phytic acid modulates the toxic effects induced by DON and FB1 on intestinal tissue.
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spelling pubmed-55984292017-09-28 Phytic acid decreases deoxynivalenol and fumonisin B1-induced changes on swine jejunal explants da Silva, Elisângela Olegário Gerez, Juliana Rubira do Carmo Drape, Thalisie Bracarense, Ana Paula F.R.L. Toxicol Rep Article The purpose of the present study was to investigate the effects of phytic acid (IP6) on morphological and immunohistochemical parameters on intestinal explants exposed to deoxynivalenol (DON) and fumonisin B1 (FB1). The jejunal explants were exposed for 4 h to different treatments: control, DON (10 μM), DON plus 2.5 mM or 5 mM IP6, FB1 (70 μM), and FB1 plus 2.5 mM or 5 mM IP6. Both mycotoxins induced significant intestinal lesions and decreased villi height. The presence of 2.5 mM and 5 mM IP6 significantly inhibited the morphological changes caused by the mycotoxins. DON induced a significant increase in caspase-3 (83%) and cyclooxygenase-2 (71.3%) expression compared with the control. The presence of 5 mM IP6 induced a significant decrease in caspase-3 (43.7%) and Cox-2 (48%) expression compared with the DON group. FB1 induced a significant increase in caspase-3 expression (47%) compared to the control, whereas IP6 induced no significant change in this expression. A significant decrease in cell proliferation was observed when explants were exposed to 5 mM of IP6 in comparison with the DON and FB1 groups. The present data provide evidence that phytic acid modulates the toxic effects induced by DON and FB1 on intestinal tissue. Elsevier 2014-05-29 /pmc/articles/PMC5598429/ /pubmed/28962245 http://dx.doi.org/10.1016/j.toxrep.2014.05.001 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Article
da Silva, Elisângela Olegário
Gerez, Juliana Rubira
do Carmo Drape, Thalisie
Bracarense, Ana Paula F.R.L.
Phytic acid decreases deoxynivalenol and fumonisin B1-induced changes on swine jejunal explants
title Phytic acid decreases deoxynivalenol and fumonisin B1-induced changes on swine jejunal explants
title_full Phytic acid decreases deoxynivalenol and fumonisin B1-induced changes on swine jejunal explants
title_fullStr Phytic acid decreases deoxynivalenol and fumonisin B1-induced changes on swine jejunal explants
title_full_unstemmed Phytic acid decreases deoxynivalenol and fumonisin B1-induced changes on swine jejunal explants
title_short Phytic acid decreases deoxynivalenol and fumonisin B1-induced changes on swine jejunal explants
title_sort phytic acid decreases deoxynivalenol and fumonisin b1-induced changes on swine jejunal explants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598429/
https://www.ncbi.nlm.nih.gov/pubmed/28962245
http://dx.doi.org/10.1016/j.toxrep.2014.05.001
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