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Effects of chromic chloride on chick embryo fibroblast viability
The objective of this study is to evaluate the effects of chromic chloride (CrCl(3)) on chick embryo fibroblast (CEF) viability. The cells were incubated with CrCl(3) (0.02, 0.1, 0.5, 2.5, 12.5, and 62.5 μM), and the viability was determined using MTT assay, morphological detection and flow cytometr...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598472/ https://www.ncbi.nlm.nih.gov/pubmed/28962390 http://dx.doi.org/10.1016/j.toxrep.2015.03.007 |
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author | Liu, Mingchao Liu, Yanhan Cheng, Ziqiang Liu, Jianzhu Chai, Tongjie |
author_facet | Liu, Mingchao Liu, Yanhan Cheng, Ziqiang Liu, Jianzhu Chai, Tongjie |
author_sort | Liu, Mingchao |
collection | PubMed |
description | The objective of this study is to evaluate the effects of chromic chloride (CrCl(3)) on chick embryo fibroblast (CEF) viability. The cells were incubated with CrCl(3) (0.02, 0.1, 0.5, 2.5, 12.5, and 62.5 μM), and the viability was determined using MTT assay, morphological detection and flow cytometry. The results show that lower concentrations of CrCl(3) (0.02, 0.1, and 0.5 μM) did not damage CEF viability. At 0.1 μM, CrCl(3) can increase CEF viability (P < 0.05). However, at higher concentrations of CrCl(3) (2.5, 12.5, and 62.5 μM), the number of apoptotic and necrotic cells (P < 0.01) and intracellular reactive oxygen species (P < 0.01) increased. In addition, decreased mitochondrial membrane potential (P < 0.01) and enhanced intracellular calcium levels (P < 0.01) were observed after the exposure. Moreover, apoptotic morphological changes induced by these processes in CEF were confirmed using Hoechst 33258 staining. Cell death induced by higher concentrations of CrCl(3) was caused by an apoptotic and a necrotic mechanism, whereas the main mechanism of oxidative stress and induced mitochondrial dysfunction was apoptotic death. The induced apoptotic death in CEF is concentration- and time-dependent. |
format | Online Article Text |
id | pubmed-5598472 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-55984722017-09-28 Effects of chromic chloride on chick embryo fibroblast viability Liu, Mingchao Liu, Yanhan Cheng, Ziqiang Liu, Jianzhu Chai, Tongjie Toxicol Rep Article The objective of this study is to evaluate the effects of chromic chloride (CrCl(3)) on chick embryo fibroblast (CEF) viability. The cells were incubated with CrCl(3) (0.02, 0.1, 0.5, 2.5, 12.5, and 62.5 μM), and the viability was determined using MTT assay, morphological detection and flow cytometry. The results show that lower concentrations of CrCl(3) (0.02, 0.1, and 0.5 μM) did not damage CEF viability. At 0.1 μM, CrCl(3) can increase CEF viability (P < 0.05). However, at higher concentrations of CrCl(3) (2.5, 12.5, and 62.5 μM), the number of apoptotic and necrotic cells (P < 0.01) and intracellular reactive oxygen species (P < 0.01) increased. In addition, decreased mitochondrial membrane potential (P < 0.01) and enhanced intracellular calcium levels (P < 0.01) were observed after the exposure. Moreover, apoptotic morphological changes induced by these processes in CEF were confirmed using Hoechst 33258 staining. Cell death induced by higher concentrations of CrCl(3) was caused by an apoptotic and a necrotic mechanism, whereas the main mechanism of oxidative stress and induced mitochondrial dysfunction was apoptotic death. The induced apoptotic death in CEF is concentration- and time-dependent. Elsevier 2015-03-23 /pmc/articles/PMC5598472/ /pubmed/28962390 http://dx.doi.org/10.1016/j.toxrep.2015.03.007 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Liu, Mingchao Liu, Yanhan Cheng, Ziqiang Liu, Jianzhu Chai, Tongjie Effects of chromic chloride on chick embryo fibroblast viability |
title | Effects of chromic chloride on chick embryo fibroblast viability |
title_full | Effects of chromic chloride on chick embryo fibroblast viability |
title_fullStr | Effects of chromic chloride on chick embryo fibroblast viability |
title_full_unstemmed | Effects of chromic chloride on chick embryo fibroblast viability |
title_short | Effects of chromic chloride on chick embryo fibroblast viability |
title_sort | effects of chromic chloride on chick embryo fibroblast viability |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598472/ https://www.ncbi.nlm.nih.gov/pubmed/28962390 http://dx.doi.org/10.1016/j.toxrep.2015.03.007 |
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