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Effects of chromic chloride on chick embryo fibroblast viability

The objective of this study is to evaluate the effects of chromic chloride (CrCl(3)) on chick embryo fibroblast (CEF) viability. The cells were incubated with CrCl(3) (0.02, 0.1, 0.5, 2.5, 12.5, and 62.5 μM), and the viability was determined using MTT assay, morphological detection and flow cytometr...

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Detalles Bibliográficos
Autores principales: Liu, Mingchao, Liu, Yanhan, Cheng, Ziqiang, Liu, Jianzhu, Chai, Tongjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598472/
https://www.ncbi.nlm.nih.gov/pubmed/28962390
http://dx.doi.org/10.1016/j.toxrep.2015.03.007
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author Liu, Mingchao
Liu, Yanhan
Cheng, Ziqiang
Liu, Jianzhu
Chai, Tongjie
author_facet Liu, Mingchao
Liu, Yanhan
Cheng, Ziqiang
Liu, Jianzhu
Chai, Tongjie
author_sort Liu, Mingchao
collection PubMed
description The objective of this study is to evaluate the effects of chromic chloride (CrCl(3)) on chick embryo fibroblast (CEF) viability. The cells were incubated with CrCl(3) (0.02, 0.1, 0.5, 2.5, 12.5, and 62.5 μM), and the viability was determined using MTT assay, morphological detection and flow cytometry. The results show that lower concentrations of CrCl(3) (0.02, 0.1, and 0.5 μM) did not damage CEF viability. At 0.1 μM, CrCl(3) can increase CEF viability (P < 0.05). However, at higher concentrations of CrCl(3) (2.5, 12.5, and 62.5 μM), the number of apoptotic and necrotic cells (P < 0.01) and intracellular reactive oxygen species (P < 0.01) increased. In addition, decreased mitochondrial membrane potential (P < 0.01) and enhanced intracellular calcium levels (P < 0.01) were observed after the exposure. Moreover, apoptotic morphological changes induced by these processes in CEF were confirmed using Hoechst 33258 staining. Cell death induced by higher concentrations of CrCl(3) was caused by an apoptotic and a necrotic mechanism, whereas the main mechanism of oxidative stress and induced mitochondrial dysfunction was apoptotic death. The induced apoptotic death in CEF is concentration- and time-dependent.
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spelling pubmed-55984722017-09-28 Effects of chromic chloride on chick embryo fibroblast viability Liu, Mingchao Liu, Yanhan Cheng, Ziqiang Liu, Jianzhu Chai, Tongjie Toxicol Rep Article The objective of this study is to evaluate the effects of chromic chloride (CrCl(3)) on chick embryo fibroblast (CEF) viability. The cells were incubated with CrCl(3) (0.02, 0.1, 0.5, 2.5, 12.5, and 62.5 μM), and the viability was determined using MTT assay, morphological detection and flow cytometry. The results show that lower concentrations of CrCl(3) (0.02, 0.1, and 0.5 μM) did not damage CEF viability. At 0.1 μM, CrCl(3) can increase CEF viability (P < 0.05). However, at higher concentrations of CrCl(3) (2.5, 12.5, and 62.5 μM), the number of apoptotic and necrotic cells (P < 0.01) and intracellular reactive oxygen species (P < 0.01) increased. In addition, decreased mitochondrial membrane potential (P < 0.01) and enhanced intracellular calcium levels (P < 0.01) were observed after the exposure. Moreover, apoptotic morphological changes induced by these processes in CEF were confirmed using Hoechst 33258 staining. Cell death induced by higher concentrations of CrCl(3) was caused by an apoptotic and a necrotic mechanism, whereas the main mechanism of oxidative stress and induced mitochondrial dysfunction was apoptotic death. The induced apoptotic death in CEF is concentration- and time-dependent. Elsevier 2015-03-23 /pmc/articles/PMC5598472/ /pubmed/28962390 http://dx.doi.org/10.1016/j.toxrep.2015.03.007 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Liu, Mingchao
Liu, Yanhan
Cheng, Ziqiang
Liu, Jianzhu
Chai, Tongjie
Effects of chromic chloride on chick embryo fibroblast viability
title Effects of chromic chloride on chick embryo fibroblast viability
title_full Effects of chromic chloride on chick embryo fibroblast viability
title_fullStr Effects of chromic chloride on chick embryo fibroblast viability
title_full_unstemmed Effects of chromic chloride on chick embryo fibroblast viability
title_short Effects of chromic chloride on chick embryo fibroblast viability
title_sort effects of chromic chloride on chick embryo fibroblast viability
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598472/
https://www.ncbi.nlm.nih.gov/pubmed/28962390
http://dx.doi.org/10.1016/j.toxrep.2015.03.007
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