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In vitro analysis of the phytotoxic and genotoxic potential of Aligarh wastewater and Mathura refinery wastewater

Present report deals with the phytotoxicity and genotoxicity of Mathura refinery wastewater and Aligarh wastewater of Northern India. The IC(50) value in Allium cepa root growth inhibition test was recorded to be 0.14X and 0.10X for Mathura refinery and Aligarh industrial wastewaters, respectively....

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Autores principales: Fazili, Naveed Ahmad, Ahmad, Masood
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598512/
https://www.ncbi.nlm.nih.gov/pubmed/28962311
http://dx.doi.org/10.1016/j.toxrep.2014.06.004
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author Fazili, Naveed Ahmad
Ahmad, Masood
author_facet Fazili, Naveed Ahmad
Ahmad, Masood
author_sort Fazili, Naveed Ahmad
collection PubMed
description Present report deals with the phytotoxicity and genotoxicity of Mathura refinery wastewater and Aligarh wastewater of Northern India. The IC(50) value in Allium cepa root growth inhibition test was recorded to be 0.14X and 0.10X for Mathura refinery and Aligarh industrial wastewaters, respectively. Significant decline in the survival of various Escherichia coli K12 DNA repair defective mutants was observed when the tester strains were exposed to the aforementioned samples. The order of sensitivity was invariably as: AB1157 (wild type) < AB2494 (lexA mutant) < AB2463 (recA mutant) < AB2480 (uvrA recA double mutant). These results suggested a significant amount of DNA damage within the bacterial cells exposed to test wastewaters. A. cepa genotoxicity test also demonstrated a considerable amount of chromosomal damage of A. cepa brought about by the test samples. The aberration index (A.I.) for Aligarh wastewater and refinery wastewater was recorded to be 11.2% and 14.7%, respectively, whereas the aquaguard mineral water serving as negative control displayed the A.I. value to be 2.6%. Interestingly, genotoxicity of both industrial wastewaters was reduced to a remarkable extent in presence of mannitol, the hydroxyl radical scavenger. Present study clearly indicated a distinct pattern of the chromosomal aberrations showing predominantly stickiness and stray chromosomes in case of AWW while clumping and stickiness in case of RWW, thereby affirming the genotoxicity of both test waters.
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spelling pubmed-55985122017-09-28 In vitro analysis of the phytotoxic and genotoxic potential of Aligarh wastewater and Mathura refinery wastewater Fazili, Naveed Ahmad Ahmad, Masood Toxicol Rep Article Present report deals with the phytotoxicity and genotoxicity of Mathura refinery wastewater and Aligarh wastewater of Northern India. The IC(50) value in Allium cepa root growth inhibition test was recorded to be 0.14X and 0.10X for Mathura refinery and Aligarh industrial wastewaters, respectively. Significant decline in the survival of various Escherichia coli K12 DNA repair defective mutants was observed when the tester strains were exposed to the aforementioned samples. The order of sensitivity was invariably as: AB1157 (wild type) < AB2494 (lexA mutant) < AB2463 (recA mutant) < AB2480 (uvrA recA double mutant). These results suggested a significant amount of DNA damage within the bacterial cells exposed to test wastewaters. A. cepa genotoxicity test also demonstrated a considerable amount of chromosomal damage of A. cepa brought about by the test samples. The aberration index (A.I.) for Aligarh wastewater and refinery wastewater was recorded to be 11.2% and 14.7%, respectively, whereas the aquaguard mineral water serving as negative control displayed the A.I. value to be 2.6%. Interestingly, genotoxicity of both industrial wastewaters was reduced to a remarkable extent in presence of mannitol, the hydroxyl radical scavenger. Present study clearly indicated a distinct pattern of the chromosomal aberrations showing predominantly stickiness and stray chromosomes in case of AWW while clumping and stickiness in case of RWW, thereby affirming the genotoxicity of both test waters. Elsevier 2014-06-27 /pmc/articles/PMC5598512/ /pubmed/28962311 http://dx.doi.org/10.1016/j.toxrep.2014.06.004 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Article
Fazili, Naveed Ahmad
Ahmad, Masood
In vitro analysis of the phytotoxic and genotoxic potential of Aligarh wastewater and Mathura refinery wastewater
title In vitro analysis of the phytotoxic and genotoxic potential of Aligarh wastewater and Mathura refinery wastewater
title_full In vitro analysis of the phytotoxic and genotoxic potential of Aligarh wastewater and Mathura refinery wastewater
title_fullStr In vitro analysis of the phytotoxic and genotoxic potential of Aligarh wastewater and Mathura refinery wastewater
title_full_unstemmed In vitro analysis of the phytotoxic and genotoxic potential of Aligarh wastewater and Mathura refinery wastewater
title_short In vitro analysis of the phytotoxic and genotoxic potential of Aligarh wastewater and Mathura refinery wastewater
title_sort in vitro analysis of the phytotoxic and genotoxic potential of aligarh wastewater and mathura refinery wastewater
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598512/
https://www.ncbi.nlm.nih.gov/pubmed/28962311
http://dx.doi.org/10.1016/j.toxrep.2014.06.004
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