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Hematological and biochemical effects of sub-chronic artesunate exposure in rats

Artesunate is a potent and rapidly acting blood schizontocide used to treat chloroquine resistant malaria. Artesunate has been reported to cause embryo, reduced reproductive capacity, hepatotoxicity, neurotoxicity and hematological abnormalities. Previously toxicity studies on artesunate have been d...

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Autores principales: Bigoniya, Papiya, Sahu, Taranginee, Tiwari, Vikalp
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598518/
https://www.ncbi.nlm.nih.gov/pubmed/28962361
http://dx.doi.org/10.1016/j.toxrep.2015.01.007
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author Bigoniya, Papiya
Sahu, Taranginee
Tiwari, Vikalp
author_facet Bigoniya, Papiya
Sahu, Taranginee
Tiwari, Vikalp
author_sort Bigoniya, Papiya
collection PubMed
description Artesunate is a potent and rapidly acting blood schizontocide used to treat chloroquine resistant malaria. Artesunate has been reported to cause embryo, reduced reproductive capacity, hepatotoxicity, neurotoxicity and hematological abnormalities. Previously toxicity studies on artesunate have been done in 2–10 mg/kg dose range mostly for 7 days, scientific studies on sub-chronic exposure of artesunate is not been reported so for. The present study evaluates sub-chronic safety profile of artesunate on 45 days oral administration at 2, 4 and 8 mg/kg/day. Authentication of artesunate has been done by color test, pH, melting point, loss on drying, UV(max), TLC and HPLC study. Artesunate has non-significant effect on liver and kidney weight. Serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), serum alkaline phosphate (ALP), cholesterol (TC), triglyceride (TG), total protein, albumin, bilirubin, creatinine, urea and glucose content were estimated after 45 days treatment along with hematological screening. Artesunate treatment for 45 days significantly increased (p < 0.05–0.001) SGOT, SGPT, ALP, TC, TG, total bilirubin, glucose level at 8 mg/kg/day dose. It has non-significant effect on serum total protein, albumin, creatinine and urea. Hemoglobin, total RBC, platelet, lymphocytes, basophil, mean cell volume and mean corpuscular hemoglobin concentration have not changed but total WBC, neutrophil, eosinophil, packed cell volume and mean cell hemoglobin were increased significantly (p < 0.01) at 8 mg/kg/day dose. Artesunate treatment at 4 and 8 mg/kg/day showed sinusoidal dilation, cytoplasmic vaculation, focal necrosis, sinusoidal congestion and extensive inflammatory changes, whereas kidney was free of any deleterious effect. CONCLUSION: Sub-chronic exposure of artesunate at 8 mg/kg/day dose for 45 days period cause hepatic damage along with hematological abnormalities signifying safety concern.
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spelling pubmed-55985182017-09-28 Hematological and biochemical effects of sub-chronic artesunate exposure in rats Bigoniya, Papiya Sahu, Taranginee Tiwari, Vikalp Toxicol Rep Article Artesunate is a potent and rapidly acting blood schizontocide used to treat chloroquine resistant malaria. Artesunate has been reported to cause embryo, reduced reproductive capacity, hepatotoxicity, neurotoxicity and hematological abnormalities. Previously toxicity studies on artesunate have been done in 2–10 mg/kg dose range mostly for 7 days, scientific studies on sub-chronic exposure of artesunate is not been reported so for. The present study evaluates sub-chronic safety profile of artesunate on 45 days oral administration at 2, 4 and 8 mg/kg/day. Authentication of artesunate has been done by color test, pH, melting point, loss on drying, UV(max), TLC and HPLC study. Artesunate has non-significant effect on liver and kidney weight. Serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), serum alkaline phosphate (ALP), cholesterol (TC), triglyceride (TG), total protein, albumin, bilirubin, creatinine, urea and glucose content were estimated after 45 days treatment along with hematological screening. Artesunate treatment for 45 days significantly increased (p < 0.05–0.001) SGOT, SGPT, ALP, TC, TG, total bilirubin, glucose level at 8 mg/kg/day dose. It has non-significant effect on serum total protein, albumin, creatinine and urea. Hemoglobin, total RBC, platelet, lymphocytes, basophil, mean cell volume and mean corpuscular hemoglobin concentration have not changed but total WBC, neutrophil, eosinophil, packed cell volume and mean cell hemoglobin were increased significantly (p < 0.01) at 8 mg/kg/day dose. Artesunate treatment at 4 and 8 mg/kg/day showed sinusoidal dilation, cytoplasmic vaculation, focal necrosis, sinusoidal congestion and extensive inflammatory changes, whereas kidney was free of any deleterious effect. CONCLUSION: Sub-chronic exposure of artesunate at 8 mg/kg/day dose for 45 days period cause hepatic damage along with hematological abnormalities signifying safety concern. Elsevier 2015-01-25 /pmc/articles/PMC5598518/ /pubmed/28962361 http://dx.doi.org/10.1016/j.toxrep.2015.01.007 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Bigoniya, Papiya
Sahu, Taranginee
Tiwari, Vikalp
Hematological and biochemical effects of sub-chronic artesunate exposure in rats
title Hematological and biochemical effects of sub-chronic artesunate exposure in rats
title_full Hematological and biochemical effects of sub-chronic artesunate exposure in rats
title_fullStr Hematological and biochemical effects of sub-chronic artesunate exposure in rats
title_full_unstemmed Hematological and biochemical effects of sub-chronic artesunate exposure in rats
title_short Hematological and biochemical effects of sub-chronic artesunate exposure in rats
title_sort hematological and biochemical effects of sub-chronic artesunate exposure in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598518/
https://www.ncbi.nlm.nih.gov/pubmed/28962361
http://dx.doi.org/10.1016/j.toxrep.2015.01.007
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