Generation of a serum free CHO DG44 cell line stably producing a broadly protective anti-influenza virus monoclonal antibody

Because of the broad neutralization and in vivo protection across influenza A and influenza B virus strains, monoclonal antibody CR9114 is widely used in influenza virus research as a positive control in many experiments. To produce amounts sufficient for the demand requires regular transient transf...

Descripción completa

Detalles Bibliográficos
Autores principales: Chromikova, Veronika, Zaragoza, Maria A., Krammer, Florian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598927/
https://www.ncbi.nlm.nih.gov/pubmed/28910287
http://dx.doi.org/10.1371/journal.pone.0183315
_version_ 1783264000778174464
author Chromikova, Veronika
Zaragoza, Maria A.
Krammer, Florian
author_facet Chromikova, Veronika
Zaragoza, Maria A.
Krammer, Florian
author_sort Chromikova, Veronika
collection PubMed
description Because of the broad neutralization and in vivo protection across influenza A and influenza B virus strains, monoclonal antibody CR9114 is widely used in influenza virus research as a positive control in many experiments. To produce amounts sufficient for the demand requires regular transient transfections, resulting in varying yield as well as differing batch to batch quality. Here, we report the development of a serum-free CHO DG44 cell line, stably producing a CR9114-like antibody with a potential to become a useful influenza virus research tool.
format Online
Article
Text
id pubmed-5598927
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-55989272017-09-22 Generation of a serum free CHO DG44 cell line stably producing a broadly protective anti-influenza virus monoclonal antibody Chromikova, Veronika Zaragoza, Maria A. Krammer, Florian PLoS One Research Article Because of the broad neutralization and in vivo protection across influenza A and influenza B virus strains, monoclonal antibody CR9114 is widely used in influenza virus research as a positive control in many experiments. To produce amounts sufficient for the demand requires regular transient transfections, resulting in varying yield as well as differing batch to batch quality. Here, we report the development of a serum-free CHO DG44 cell line, stably producing a CR9114-like antibody with a potential to become a useful influenza virus research tool. Public Library of Science 2017-09-14 /pmc/articles/PMC5598927/ /pubmed/28910287 http://dx.doi.org/10.1371/journal.pone.0183315 Text en © 2017 Chromikova et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Chromikova, Veronika
Zaragoza, Maria A.
Krammer, Florian
Generation of a serum free CHO DG44 cell line stably producing a broadly protective anti-influenza virus monoclonal antibody
title Generation of a serum free CHO DG44 cell line stably producing a broadly protective anti-influenza virus monoclonal antibody
title_full Generation of a serum free CHO DG44 cell line stably producing a broadly protective anti-influenza virus monoclonal antibody
title_fullStr Generation of a serum free CHO DG44 cell line stably producing a broadly protective anti-influenza virus monoclonal antibody
title_full_unstemmed Generation of a serum free CHO DG44 cell line stably producing a broadly protective anti-influenza virus monoclonal antibody
title_short Generation of a serum free CHO DG44 cell line stably producing a broadly protective anti-influenza virus monoclonal antibody
title_sort generation of a serum free cho dg44 cell line stably producing a broadly protective anti-influenza virus monoclonal antibody
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598927/
https://www.ncbi.nlm.nih.gov/pubmed/28910287
http://dx.doi.org/10.1371/journal.pone.0183315
work_keys_str_mv AT chromikovaveronika generationofaserumfreechodg44celllinestablyproducingabroadlyprotectiveantiinfluenzavirusmonoclonalantibody
AT zaragozamariaa generationofaserumfreechodg44celllinestablyproducingabroadlyprotectiveantiinfluenzavirusmonoclonalantibody
AT krammerflorian generationofaserumfreechodg44celllinestablyproducingabroadlyprotectiveantiinfluenzavirusmonoclonalantibody

Ejemplares similares