Cargando…

Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons

Historically, room-temperature structure determination was succeeded by cryo-crystallography to mitigate radiation damage. Here, we demonstrate that serial millisecond crystallography at a synchrotron beamline equipped with high-viscosity injector and high frame-rate detector allows typical crystall...

Descripción completa

Detalles Bibliográficos
Autores principales:	Weinert, Tobias, Olieric, Natacha, Cheng, Robert, Brünle, Steffen, James, Daniel, Ozerov, Dmitry, Gashi, Dardan, Vera, Laura, Marsh, May, Jaeger, Kathrin, Dworkowski, Florian, Panepucci, Ezequiel, Basu, Shibom, Skopintsev, Petr, Doré, Andrew S., Geng, Tian, Cooke, Robert M., Liang, Mengning, Prota, Andrea E., Panneels, Valerie, Nogly, Przemyslaw, Ermler, Ulrich, Schertler, Gebhard, Hennig, Michael, Steinmetz, Michel O., Wang, Meitian, Standfuss, Jörg
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5599499/ https://www.ncbi.nlm.nih.gov/pubmed/28912485 http://dx.doi.org/10.1038/s41467-017-00630-4

Ejemplares similares

Lipidic cubic phase serial millisecond crystallography using synchrotron radiation
por: Nogly, Przemyslaw, et al.
Publicado: (2015)

Watching the release of a photopharmacological drug from tubulin using time-resolved serial crystallography
por: Wranik, Maximilian, et al.
Publicado: (2023)

In situ serial crystallography for rapid de novo membrane protein structure determination
por: Huang, Chia-Ying, et al.
Publicado: (2018)

Correction of rhodopsin serial crystallography diffraction intensities for a lattice-translocation defect
por: Rodrigues, Matthew J., et al.
Publicado: (2023)

Advances in long-wavelength native phasing at X-ray free-electron lasers
por: Nass, Karol, et al.
Publicado: (2020)

Time-resolved structural studies with serial crystallography: A new light on retinal proteins
por: Panneels, Valérie, et al.
Publicado: (2015)

Batch crystallization of rhodopsin for structural dynamics using an X-ray free-electron laser
por: Wu, Wenting, et al.
Publicado: (2015)

In meso in situ serial X-ray crystallography of soluble and membrane proteins
por: Huang, Chia-Ying, et al.
Publicado: (2015)

Low-pass spectral analysis of time-resolved serial femtosecond crystallography data
por: Casadei, Cecilia M., et al.
Publicado: (2023)

Acoustic levitation and rotation of thin films and their application for room temperature protein crystallography
por: Kepa, Michal. W., et al.
Publicado: (2022)

Publisher Correction: Acoustic levitation and rotation of thin films and their application for room temperature protein crystallography
por: Kepa, Michal. W., et al.
Publicado: (2022)

Automated data collection and real-time data analysis suite for serial synchrotron crystallography
por: Basu, Shibom, et al.
Publicado: (2019)

PRIGo: a new multi-axis goniometer for macromolecular crystallography
por: Waltersperger, Sandro, et al.
Publicado: (2015)

In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures
por: Huang, Chia-Ying, et al.
Publicado: (2016)

EIGER detector: application in macromolecular crystallography
por: Casanas, Arnau, et al.
Publicado: (2016)

A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers
por: Wranik, Maximilian, et al.
Publicado: (2023)

D3, the new diffractometer for the macromolecular crystallography beamlines of the Swiss Light Source
por: Fuchs, Martin R., et al.
Publicado: (2014)

A tool for visualizing protein motions in time-resolved crystallography
por: Wickstrand, Cecilia, et al.
Publicado: (2020)

Ultrafast structural changes direct the first molecular events of vision
por: Gruhl, Thomas, et al.
Publicado: (2023)

Low-dose in situ prelocation of protein microcrystals by 2D X-ray phase-contrast imaging for serial crystallography
por: Martiel, Isabelle, et al.
Publicado: (2020)

Crystal structure of jumping spider rhodopsin-1 as a light sensitive GPCR
por: Varma, Niranjan, et al.
Publicado: (2019)

Kilohertz serial crystallography with the JUNGFRAU detector at a fourth-generation synchrotron source
por: Leonarski, Filip, et al.
Publicado: (2023)

Pink-beam serial femtosecond crystallography for accurate structure-factor determination at an X-ray free-electron laser
por: Nass, Karol, et al.
Publicado: (2021)

X-ray fluorescence detection for serial macromolecular crystallography using a JUNGFRAU pixel detector
por: Martiel, Isabelle, et al.
Publicado: (2020)

Making routine native SAD a reality: lessons from beamline X06DA at the Swiss Light Source
por: Basu, Shibom, et al.
Publicado: (2019)

3D-printed holders for in meso in situ fixed-target serial X-ray crystallography
por: Huang, Chia-Ying, et al.
Publicado: (2020)

DA+ data acquisition and analysis software at the Swiss Light Source macromolecular crystallography beamlines
por: Wojdyla, Justyna Aleksandra, et al.
Publicado: (2018)

Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation
por: Martin-Garcia, Jose M., et al.
Publicado: (2017)

Millisecond cryo-trapping by the spitrobot crystal plunger simplifies time-resolved crystallography
por: Mehrabi, Pedram, et al.
Publicado: (2023)

Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
por: Nogly, Przemyslaw, et al.
Publicado: (2016)

Serial crystallography at synchrotrons and X-ray lasers
por: Standfuss, Jörg, et al.
Publicado: (2017)

Millisecond time-resolved serial oscillation crystallography of a blue-light photoreceptor at a synchrotron
por: Aumonier, Sylvain, et al.
Publicado: (2020)

Crystal structure of rhodopsin in complex with a mini-G(o) sheds light on the principles of G protein selectivity
por: Tsai, Ching-Ju, et al.
Publicado: (2018)

Protein crystallography with a micrometre-sized synchrotron-radiation beam
por: Moukhametzianov, Rouslan, et al.
Publicado: (2008)

Millisecond mix-and-quench crystallography (MMQX) enables time-resolved studies of PEPCK with remote data collection
por: Clinger, Jonathan A., et al.
Publicado: (2021)

Versatile microporous polymer-based supports for serial macromolecular crystallography
por: Martiel, Isabelle, et al.
Publicado: (2021)

Data-collection strategy for challenging native SAD phasing
por: Olieric, Vincent, et al.
Publicado: (2016)

Probing ligand binding of endothiapepsin by ‘temperature-resolved’ macromolecular crystallography
por: Huang, Chia-Ying, et al.
Publicado: (2022)

Room temperature structures beyond 1.5 Å by serial femtosecond crystallography
por: Schmidt, Marius, et al.
Publicado: (2015)

Crystallization Scale Preparation of a Stable GPCR Signaling Complex between Constitutively Active Rhodopsin and G-Protein
por: Maeda, Shoji, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_gvrla5gm7aq6ih67l1v8qolqlq