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Cohesins and condensins orchestrate the 4D dynamics of yeast chromosomes during the cell cycle

Duplication and segregation of chromosomes involves dynamic reorganization of their internal structure by conserved architectural proteins, including the structural maintenance of chromosomes (SMC) complexes cohesin and condensin. Despite active investigation of the roles of these factors, a genome‐...

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Autores principales: Lazar‐Stefanita, Luciana, Scolari, Vittore F, Mercy, Guillaume, Muller, Héloise, Guérin, Thomas M, Thierry, Agnès, Mozziconacci, Julien, Koszul, Romain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5599795/
https://www.ncbi.nlm.nih.gov/pubmed/28729434
http://dx.doi.org/10.15252/embj.201797342
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author Lazar‐Stefanita, Luciana
Scolari, Vittore F
Mercy, Guillaume
Muller, Héloise
Guérin, Thomas M
Thierry, Agnès
Mozziconacci, Julien
Koszul, Romain
author_facet Lazar‐Stefanita, Luciana
Scolari, Vittore F
Mercy, Guillaume
Muller, Héloise
Guérin, Thomas M
Thierry, Agnès
Mozziconacci, Julien
Koszul, Romain
author_sort Lazar‐Stefanita, Luciana
collection PubMed
description Duplication and segregation of chromosomes involves dynamic reorganization of their internal structure by conserved architectural proteins, including the structural maintenance of chromosomes (SMC) complexes cohesin and condensin. Despite active investigation of the roles of these factors, a genome‐wide view of dynamic chromosome architecture at both small and large scale during cell division is still missing. Here, we report the first comprehensive 4D analysis of the higher‐order organization of the Saccharomyces cerevisiae genome throughout the cell cycle and investigate the roles of SMC complexes in controlling structural transitions. During replication, cohesion establishment promotes numerous long‐range intra‐chromosomal contacts and correlates with the individualization of chromosomes, which culminates at metaphase. In anaphase, mitotic chromosomes are abruptly reorganized depending on mechanical forces exerted by the mitotic spindle. Formation of a condensin‐dependent loop bridging the centromere cluster with the rDNA loci suggests that condensin‐mediated forces may also directly facilitate segregation. This work therefore comprehensively recapitulates cell cycle‐dependent chromosome dynamics in a unicellular eukaryote, but also unveils new features of chromosome structural reorganization during highly conserved stages of cell division.
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spelling pubmed-55997952017-09-15 Cohesins and condensins orchestrate the 4D dynamics of yeast chromosomes during the cell cycle Lazar‐Stefanita, Luciana Scolari, Vittore F Mercy, Guillaume Muller, Héloise Guérin, Thomas M Thierry, Agnès Mozziconacci, Julien Koszul, Romain EMBO J Articles Duplication and segregation of chromosomes involves dynamic reorganization of their internal structure by conserved architectural proteins, including the structural maintenance of chromosomes (SMC) complexes cohesin and condensin. Despite active investigation of the roles of these factors, a genome‐wide view of dynamic chromosome architecture at both small and large scale during cell division is still missing. Here, we report the first comprehensive 4D analysis of the higher‐order organization of the Saccharomyces cerevisiae genome throughout the cell cycle and investigate the roles of SMC complexes in controlling structural transitions. During replication, cohesion establishment promotes numerous long‐range intra‐chromosomal contacts and correlates with the individualization of chromosomes, which culminates at metaphase. In anaphase, mitotic chromosomes are abruptly reorganized depending on mechanical forces exerted by the mitotic spindle. Formation of a condensin‐dependent loop bridging the centromere cluster with the rDNA loci suggests that condensin‐mediated forces may also directly facilitate segregation. This work therefore comprehensively recapitulates cell cycle‐dependent chromosome dynamics in a unicellular eukaryote, but also unveils new features of chromosome structural reorganization during highly conserved stages of cell division. John Wiley and Sons Inc. 2017-07-20 2017-09-15 /pmc/articles/PMC5599795/ /pubmed/28729434 http://dx.doi.org/10.15252/embj.201797342 Text en © 2017 The Authors. Published under the terms of the CC BY 4.0 license This is an open access article under the terms of the Creative Commons Attribution 4.0 (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Lazar‐Stefanita, Luciana
Scolari, Vittore F
Mercy, Guillaume
Muller, Héloise
Guérin, Thomas M
Thierry, Agnès
Mozziconacci, Julien
Koszul, Romain
Cohesins and condensins orchestrate the 4D dynamics of yeast chromosomes during the cell cycle
title Cohesins and condensins orchestrate the 4D dynamics of yeast chromosomes during the cell cycle
title_full Cohesins and condensins orchestrate the 4D dynamics of yeast chromosomes during the cell cycle
title_fullStr Cohesins and condensins orchestrate the 4D dynamics of yeast chromosomes during the cell cycle
title_full_unstemmed Cohesins and condensins orchestrate the 4D dynamics of yeast chromosomes during the cell cycle
title_short Cohesins and condensins orchestrate the 4D dynamics of yeast chromosomes during the cell cycle
title_sort cohesins and condensins orchestrate the 4d dynamics of yeast chromosomes during the cell cycle
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5599795/
https://www.ncbi.nlm.nih.gov/pubmed/28729434
http://dx.doi.org/10.15252/embj.201797342
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