Cargando…
Oil and eicosapentaenoic acid production by the diatom Phaeodactylum tricornutum cultivated outdoors in Green Wall Panel (GWP®) reactors
Phaeodactylum tricornutum is a widely studied diatom and has been proposed as a source of oil and polyunsaturated fatty acids (PUFA), particularly eicosapentaenoic acid (EPA). Recent studies indicate that lipid accumulation occurs under nutritional stress. Aim of this research was to determine how c...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5599966/ https://www.ncbi.nlm.nih.gov/pubmed/28627710 http://dx.doi.org/10.1002/bit.26353 |
_version_ | 1783264158356078592 |
---|---|
author | Rodolfi, Liliana Biondi, Natascia Guccione, Alessia Bassi, Niccolò D'Ottavio, Massimo Arganaraz, Gimena Tredici, Mario R. |
author_facet | Rodolfi, Liliana Biondi, Natascia Guccione, Alessia Bassi, Niccolò D'Ottavio, Massimo Arganaraz, Gimena Tredici, Mario R. |
author_sort | Rodolfi, Liliana |
collection | PubMed |
description | Phaeodactylum tricornutum is a widely studied diatom and has been proposed as a source of oil and polyunsaturated fatty acids (PUFA), particularly eicosapentaenoic acid (EPA). Recent studies indicate that lipid accumulation occurs under nutritional stress. Aim of this research was to determine how changes in nitrogen availability affect productivity, oil yield, and fatty acid (FA) composition of P. tricornutum UTEX 640. After preliminary laboratory trials, outdoor experiments were carried out in 40‐L GWP® reactors under different nitrogen regimes in batch. Nitrogen replete cultures achieved the highest productivity of biomass (about 18 g m(−2) d(−1)) and EPA (about 0.35 g m(−2) d(−1)), whereas nitrogen‐starved cultures achieved the highest FA productivity (about 2.6 g m(−2) d(−1)). The annual potential yield of P. tricornutum grown outdoors in GWP® reactors is 730 kg of EPA per hectare under nutrient‐replete conditions and 5,800 kg of FA per hectare under nitrogen starvation. Biotechnol. Bioeng. 2017;114: 2204–2210. © 2017 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc. |
format | Online Article Text |
id | pubmed-5599966 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-55999662017-10-02 Oil and eicosapentaenoic acid production by the diatom Phaeodactylum tricornutum cultivated outdoors in Green Wall Panel (GWP®) reactors Rodolfi, Liliana Biondi, Natascia Guccione, Alessia Bassi, Niccolò D'Ottavio, Massimo Arganaraz, Gimena Tredici, Mario R. Biotechnol Bioeng Articles Phaeodactylum tricornutum is a widely studied diatom and has been proposed as a source of oil and polyunsaturated fatty acids (PUFA), particularly eicosapentaenoic acid (EPA). Recent studies indicate that lipid accumulation occurs under nutritional stress. Aim of this research was to determine how changes in nitrogen availability affect productivity, oil yield, and fatty acid (FA) composition of P. tricornutum UTEX 640. After preliminary laboratory trials, outdoor experiments were carried out in 40‐L GWP® reactors under different nitrogen regimes in batch. Nitrogen replete cultures achieved the highest productivity of biomass (about 18 g m(−2) d(−1)) and EPA (about 0.35 g m(−2) d(−1)), whereas nitrogen‐starved cultures achieved the highest FA productivity (about 2.6 g m(−2) d(−1)). The annual potential yield of P. tricornutum grown outdoors in GWP® reactors is 730 kg of EPA per hectare under nutrient‐replete conditions and 5,800 kg of FA per hectare under nitrogen starvation. Biotechnol. Bioeng. 2017;114: 2204–2210. © 2017 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc. John Wiley and Sons Inc. 2017-07-11 2017-10 /pmc/articles/PMC5599966/ /pubmed/28627710 http://dx.doi.org/10.1002/bit.26353 Text en © 2017 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Articles Rodolfi, Liliana Biondi, Natascia Guccione, Alessia Bassi, Niccolò D'Ottavio, Massimo Arganaraz, Gimena Tredici, Mario R. Oil and eicosapentaenoic acid production by the diatom Phaeodactylum tricornutum cultivated outdoors in Green Wall Panel (GWP®) reactors |
title | Oil and eicosapentaenoic acid production by the diatom Phaeodactylum tricornutum cultivated outdoors in Green Wall Panel (GWP®) reactors |
title_full | Oil and eicosapentaenoic acid production by the diatom Phaeodactylum tricornutum cultivated outdoors in Green Wall Panel (GWP®) reactors |
title_fullStr | Oil and eicosapentaenoic acid production by the diatom Phaeodactylum tricornutum cultivated outdoors in Green Wall Panel (GWP®) reactors |
title_full_unstemmed | Oil and eicosapentaenoic acid production by the diatom Phaeodactylum tricornutum cultivated outdoors in Green Wall Panel (GWP®) reactors |
title_short | Oil and eicosapentaenoic acid production by the diatom Phaeodactylum tricornutum cultivated outdoors in Green Wall Panel (GWP®) reactors |
title_sort | oil and eicosapentaenoic acid production by the diatom phaeodactylum tricornutum cultivated outdoors in green wall panel (gwp®) reactors |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5599966/ https://www.ncbi.nlm.nih.gov/pubmed/28627710 http://dx.doi.org/10.1002/bit.26353 |
work_keys_str_mv | AT rodolfililiana oilandeicosapentaenoicacidproductionbythediatomphaeodactylumtricornutumcultivatedoutdoorsingreenwallpanelgwpreactors AT biondinatascia oilandeicosapentaenoicacidproductionbythediatomphaeodactylumtricornutumcultivatedoutdoorsingreenwallpanelgwpreactors AT guccionealessia oilandeicosapentaenoicacidproductionbythediatomphaeodactylumtricornutumcultivatedoutdoorsingreenwallpanelgwpreactors AT bassiniccolo oilandeicosapentaenoicacidproductionbythediatomphaeodactylumtricornutumcultivatedoutdoorsingreenwallpanelgwpreactors AT dottaviomassimo oilandeicosapentaenoicacidproductionbythediatomphaeodactylumtricornutumcultivatedoutdoorsingreenwallpanelgwpreactors AT arganarazgimena oilandeicosapentaenoicacidproductionbythediatomphaeodactylumtricornutumcultivatedoutdoorsingreenwallpanelgwpreactors AT tredicimarior oilandeicosapentaenoicacidproductionbythediatomphaeodactylumtricornutumcultivatedoutdoorsingreenwallpanelgwpreactors |