Investigation of chimeric reads using the MinION

Following a nanopore sequencing run of PCR products of three amplicons less than 1kb, an abundance of reads failed quality control due to template/complement mismatch. A BLAST search demonstrated that some of the failed reads mapped to two different genes -- an unexpected observation, given that PCR...

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Detalles Bibliográficos
Autores principales: White, Ruby, Pellefigues, Christophe, Ronchese, Franca, Lamiable, Olivier, Eccles, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000Research 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5600009/
https://www.ncbi.nlm.nih.gov/pubmed/28928943
http://dx.doi.org/10.12688/f1000research.11547.2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5600009/
https://www.ncbi.nlm.nih.gov/pubmed/28928943
http://dx.doi.org/10.12688/f1000research.11547.2

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