Locus-specific integration of extrachromosomal transgenes in C. elegans with the CRISPR/Cas9 system

We established a method to generate integration from extrachromosomal arrays with the CRISPR/Cas9 system. Multi-copy transgenes were integrated into the defined loci of chromosomes by this method, while a multi-copy transgene is integrated into random loci by previous methods, such as UV- and gamma-...

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Detalles Bibliográficos
Autores principales: Yoshina, Sawako, Suehiro, Yuji, Kage-Nakadai, Eriko, Mitani, Shohei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5600330/
https://www.ncbi.nlm.nih.gov/pubmed/28955808
http://dx.doi.org/10.1016/j.bbrep.2015.11.017

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5600330/
https://www.ncbi.nlm.nih.gov/pubmed/28955808
http://dx.doi.org/10.1016/j.bbrep.2015.11.017

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