Detection of microwave radiation of cytochrome CYP102 A1 solution during the enzyme reaction

Microwave radiation at 3.4–4.2 GHz frequency of the cytochrome P450 CYP102 A1 (BM3) solution was registered during the lauric acid hydroxylation reaction. The microwave radiation generation was shown to occur following the addition of electron donor NADPH to a system containing an enzyme and a subst...

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Autores principales: Ivanov, Yu.D., Malsagova, K.A., Izotov, A.A., Pleshakova, T.O., Tatur, V.Yu., Vesnin, S.G., Ivanova, N.D., Usanov, S.A., Archakov, A.I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5600332/
https://www.ncbi.nlm.nih.gov/pubmed/28955835
http://dx.doi.org/10.1016/j.bbrep.2015.12.013
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author Ivanov, Yu.D.
Malsagova, K.A.
Izotov, A.A.
Pleshakova, T.O.
Tatur, V.Yu.
Vesnin, S.G.
Ivanova, N.D.
Usanov, S.A.
Archakov, A.I.
author_facet Ivanov, Yu.D.
Malsagova, K.A.
Izotov, A.A.
Pleshakova, T.O.
Tatur, V.Yu.
Vesnin, S.G.
Ivanova, N.D.
Usanov, S.A.
Archakov, A.I.
author_sort Ivanov, Yu.D.
collection PubMed
description Microwave radiation at 3.4–4.2 GHz frequency of the cytochrome P450 CYP102 A1 (BM3) solution was registered during the lauric acid hydroxylation reaction. The microwave radiation generation was shown to occur following the addition of electron donor NADPH to a system containing an enzyme and a substrate. The radiation occurs for the enzyme solutions with enzyme concentrations of 10(−8) and 10(−9) М. The microwave radiation effect elicited by the aqueous enzyme solution was observed for the first time. The results obtained can be used to elaborate a new approach to enzyme systems research, including studying of the mechanism of interaction of a functioning enzyme system with microenvironment.
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spelling pubmed-56003322017-09-27 Detection of microwave radiation of cytochrome CYP102 A1 solution during the enzyme reaction Ivanov, Yu.D. Malsagova, K.A. Izotov, A.A. Pleshakova, T.O. Tatur, V.Yu. Vesnin, S.G. Ivanova, N.D. Usanov, S.A. Archakov, A.I. Biochem Biophys Rep Research Article Microwave radiation at 3.4–4.2 GHz frequency of the cytochrome P450 CYP102 A1 (BM3) solution was registered during the lauric acid hydroxylation reaction. The microwave radiation generation was shown to occur following the addition of electron donor NADPH to a system containing an enzyme and a substrate. The radiation occurs for the enzyme solutions with enzyme concentrations of 10(−8) and 10(−9) М. The microwave radiation effect elicited by the aqueous enzyme solution was observed for the first time. The results obtained can be used to elaborate a new approach to enzyme systems research, including studying of the mechanism of interaction of a functioning enzyme system with microenvironment. Elsevier 2015-12-31 /pmc/articles/PMC5600332/ /pubmed/28955835 http://dx.doi.org/10.1016/j.bbrep.2015.12.013 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Ivanov, Yu.D.
Malsagova, K.A.
Izotov, A.A.
Pleshakova, T.O.
Tatur, V.Yu.
Vesnin, S.G.
Ivanova, N.D.
Usanov, S.A.
Archakov, A.I.
Detection of microwave radiation of cytochrome CYP102 A1 solution during the enzyme reaction
title Detection of microwave radiation of cytochrome CYP102 A1 solution during the enzyme reaction
title_full Detection of microwave radiation of cytochrome CYP102 A1 solution during the enzyme reaction
title_fullStr Detection of microwave radiation of cytochrome CYP102 A1 solution during the enzyme reaction
title_full_unstemmed Detection of microwave radiation of cytochrome CYP102 A1 solution during the enzyme reaction
title_short Detection of microwave radiation of cytochrome CYP102 A1 solution during the enzyme reaction
title_sort detection of microwave radiation of cytochrome cyp102 a1 solution during the enzyme reaction
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5600332/
https://www.ncbi.nlm.nih.gov/pubmed/28955835
http://dx.doi.org/10.1016/j.bbrep.2015.12.013
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