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Deleterious effect of Usutu virus on human neural cells

In the last decade, the number of emerging Flaviviruses described worldwide has increased considerably. Among them Zika virus (ZIKV) and Usutu virus (USUV) are African mosquito-borne viruses that recently emerged. Recently, ZIKV has been intensely studied due to major outbreaks associated with neona...

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Autores principales: Salinas, Sara, Constant, Orianne, Desmetz, Caroline, Barthelemy, Jonathan, Lemaitre, Jean-Marc, Milhavet, Ollivier, Nagot, Nicolas, Foulongne, Vincent, Perrin, Florence E., Saiz, Juan-Carlos, Lecollinet, Sylvie, Van de Perre, Philippe, Simonin, Yannick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5600396/
https://www.ncbi.nlm.nih.gov/pubmed/28873445
http://dx.doi.org/10.1371/journal.pntd.0005913
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author Salinas, Sara
Constant, Orianne
Desmetz, Caroline
Barthelemy, Jonathan
Lemaitre, Jean-Marc
Milhavet, Ollivier
Nagot, Nicolas
Foulongne, Vincent
Perrin, Florence E.
Saiz, Juan-Carlos
Lecollinet, Sylvie
Van de Perre, Philippe
Simonin, Yannick
author_facet Salinas, Sara
Constant, Orianne
Desmetz, Caroline
Barthelemy, Jonathan
Lemaitre, Jean-Marc
Milhavet, Ollivier
Nagot, Nicolas
Foulongne, Vincent
Perrin, Florence E.
Saiz, Juan-Carlos
Lecollinet, Sylvie
Van de Perre, Philippe
Simonin, Yannick
author_sort Salinas, Sara
collection PubMed
description In the last decade, the number of emerging Flaviviruses described worldwide has increased considerably. Among them Zika virus (ZIKV) and Usutu virus (USUV) are African mosquito-borne viruses that recently emerged. Recently, ZIKV has been intensely studied due to major outbreaks associated with neonatal death and birth defects, as well as neurological symptoms. USUV pathogenesis remains largely unexplored, despite significant human and veterinary associated disorders. Circulation of USUV in Africa was documented more than 50 years ago, and it emerged in Europe two decades ago, causing massive bird mortality. More recently, USUV has been described to be associated with neurological disorders in humans such as encephalitis and meningoencephalitis, highlighting USUV as a potential health threat. The aim of this study was to evaluate the ability of USUV to infect neuronal cells. Our results indicate that USUV efficiently infects neurons, astrocytes, microglia and IPSc-derived human neuronal stem cells. When compared to ZIKV, USUV led to a higher infection rate, viral production, as well as stronger cell death and anti-viral response. Our results highlight the need to better characterize the physiopathology related to USUV infection in order to anticipate the potential threat of USUV emergence.
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spelling pubmed-56003962017-09-22 Deleterious effect of Usutu virus on human neural cells Salinas, Sara Constant, Orianne Desmetz, Caroline Barthelemy, Jonathan Lemaitre, Jean-Marc Milhavet, Ollivier Nagot, Nicolas Foulongne, Vincent Perrin, Florence E. Saiz, Juan-Carlos Lecollinet, Sylvie Van de Perre, Philippe Simonin, Yannick PLoS Negl Trop Dis Research Article In the last decade, the number of emerging Flaviviruses described worldwide has increased considerably. Among them Zika virus (ZIKV) and Usutu virus (USUV) are African mosquito-borne viruses that recently emerged. Recently, ZIKV has been intensely studied due to major outbreaks associated with neonatal death and birth defects, as well as neurological symptoms. USUV pathogenesis remains largely unexplored, despite significant human and veterinary associated disorders. Circulation of USUV in Africa was documented more than 50 years ago, and it emerged in Europe two decades ago, causing massive bird mortality. More recently, USUV has been described to be associated with neurological disorders in humans such as encephalitis and meningoencephalitis, highlighting USUV as a potential health threat. The aim of this study was to evaluate the ability of USUV to infect neuronal cells. Our results indicate that USUV efficiently infects neurons, astrocytes, microglia and IPSc-derived human neuronal stem cells. When compared to ZIKV, USUV led to a higher infection rate, viral production, as well as stronger cell death and anti-viral response. Our results highlight the need to better characterize the physiopathology related to USUV infection in order to anticipate the potential threat of USUV emergence. Public Library of Science 2017-09-05 /pmc/articles/PMC5600396/ /pubmed/28873445 http://dx.doi.org/10.1371/journal.pntd.0005913 Text en © 2017 Salinas et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Salinas, Sara
Constant, Orianne
Desmetz, Caroline
Barthelemy, Jonathan
Lemaitre, Jean-Marc
Milhavet, Ollivier
Nagot, Nicolas
Foulongne, Vincent
Perrin, Florence E.
Saiz, Juan-Carlos
Lecollinet, Sylvie
Van de Perre, Philippe
Simonin, Yannick
Deleterious effect of Usutu virus on human neural cells
title Deleterious effect of Usutu virus on human neural cells
title_full Deleterious effect of Usutu virus on human neural cells
title_fullStr Deleterious effect of Usutu virus on human neural cells
title_full_unstemmed Deleterious effect of Usutu virus on human neural cells
title_short Deleterious effect of Usutu virus on human neural cells
title_sort deleterious effect of usutu virus on human neural cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5600396/
https://www.ncbi.nlm.nih.gov/pubmed/28873445
http://dx.doi.org/10.1371/journal.pntd.0005913
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