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Hydrogen Sulfide Facilitates the Impaired Sensitivity of Carotid Sinus Baroreflex in Rats with Vascular Calcification
Arterial baroreflex is a general mechanism maintaining cardiovascular homeostasis; its sensitivity is reduced in vascular calcification (VC). Hydrogen sulfide (H(2)S) treatment facilitates baroreflexive sensitivity in normal and hypertensive rats. Here, we aimed to detect the effect of H(2)S on baro...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5601057/ https://www.ncbi.nlm.nih.gov/pubmed/28955233 http://dx.doi.org/10.3389/fphar.2017.00629 |
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author | Li, Hui Teng, Xu Yang, Rui Guo, Qi Xue, Hongmei Xiao, Lin Duan, Xiaocui Tian, Danyang Feng, Xiaohong Wu, Yuming |
author_facet | Li, Hui Teng, Xu Yang, Rui Guo, Qi Xue, Hongmei Xiao, Lin Duan, Xiaocui Tian, Danyang Feng, Xiaohong Wu, Yuming |
author_sort | Li, Hui |
collection | PubMed |
description | Arterial baroreflex is a general mechanism maintaining cardiovascular homeostasis; its sensitivity is reduced in vascular calcification (VC). Hydrogen sulfide (H(2)S) treatment facilitates baroreflexive sensitivity in normal and hypertensive rats. Here, we aimed to detect the effect of H(2)S on baroreflexive sensitivity in rats with VC. The rat VC model was induced by vitamin D(3) plus nicotine for 4 weeks. The sensitivity of baroreflex was detected by perfusing the isolated carotid sinus. VC was assessed by hematoxylin and eosin (H&E) staining, Ca(2+) content and alkaline phosphatase (ALP) activity. Protein levels were detected by western blot analysis. Vitamin D(3) plus nicotine induced structural disorder and elevated Ca(2+) content in the aortic and carotid arterial wall and increased plasma ALP activity. In the calcified aorta and carotid artery, protein levels of contractile phenotype markers of vascular smooth muscle cells (VSMCs) were downregulated and that of osteoblast-like phenotype markers and endoplasmic reticulum stress (ERS) markers were upregulated. NaHS treatment ameliorated the histologic disorder and Ca(2+) content in the calcified aorta and carotid artery, inhibited the elevated plasma ALP activity, and prevented the transformation of the VSMC phenotype and activation of ERS in rats with VC. Chronic NaHS treatment prevented the impairment of the baroreflex sensitivity and acute NaHS treatment dose-dependently improved the sensitivity in rats with VC. Our results suggested that H(2)S could directly facilitate the impairment of baroreflex in rats with VC and ameliorate VC, which might provide new target and strategy for regulation of the baroreflex and therapy of VC. |
format | Online Article Text |
id | pubmed-5601057 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-56010572017-09-27 Hydrogen Sulfide Facilitates the Impaired Sensitivity of Carotid Sinus Baroreflex in Rats with Vascular Calcification Li, Hui Teng, Xu Yang, Rui Guo, Qi Xue, Hongmei Xiao, Lin Duan, Xiaocui Tian, Danyang Feng, Xiaohong Wu, Yuming Front Pharmacol Pharmacology Arterial baroreflex is a general mechanism maintaining cardiovascular homeostasis; its sensitivity is reduced in vascular calcification (VC). Hydrogen sulfide (H(2)S) treatment facilitates baroreflexive sensitivity in normal and hypertensive rats. Here, we aimed to detect the effect of H(2)S on baroreflexive sensitivity in rats with VC. The rat VC model was induced by vitamin D(3) plus nicotine for 4 weeks. The sensitivity of baroreflex was detected by perfusing the isolated carotid sinus. VC was assessed by hematoxylin and eosin (H&E) staining, Ca(2+) content and alkaline phosphatase (ALP) activity. Protein levels were detected by western blot analysis. Vitamin D(3) plus nicotine induced structural disorder and elevated Ca(2+) content in the aortic and carotid arterial wall and increased plasma ALP activity. In the calcified aorta and carotid artery, protein levels of contractile phenotype markers of vascular smooth muscle cells (VSMCs) were downregulated and that of osteoblast-like phenotype markers and endoplasmic reticulum stress (ERS) markers were upregulated. NaHS treatment ameliorated the histologic disorder and Ca(2+) content in the calcified aorta and carotid artery, inhibited the elevated plasma ALP activity, and prevented the transformation of the VSMC phenotype and activation of ERS in rats with VC. Chronic NaHS treatment prevented the impairment of the baroreflex sensitivity and acute NaHS treatment dose-dependently improved the sensitivity in rats with VC. Our results suggested that H(2)S could directly facilitate the impairment of baroreflex in rats with VC and ameliorate VC, which might provide new target and strategy for regulation of the baroreflex and therapy of VC. Frontiers Media S.A. 2017-09-12 /pmc/articles/PMC5601057/ /pubmed/28955233 http://dx.doi.org/10.3389/fphar.2017.00629 Text en Copyright © 2017 Li, Teng, Yang, Guo, Xue, Xiao, Duan, Tian, Feng and Wu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Li, Hui Teng, Xu Yang, Rui Guo, Qi Xue, Hongmei Xiao, Lin Duan, Xiaocui Tian, Danyang Feng, Xiaohong Wu, Yuming Hydrogen Sulfide Facilitates the Impaired Sensitivity of Carotid Sinus Baroreflex in Rats with Vascular Calcification |
title | Hydrogen Sulfide Facilitates the Impaired Sensitivity of Carotid Sinus Baroreflex in Rats with Vascular Calcification |
title_full | Hydrogen Sulfide Facilitates the Impaired Sensitivity of Carotid Sinus Baroreflex in Rats with Vascular Calcification |
title_fullStr | Hydrogen Sulfide Facilitates the Impaired Sensitivity of Carotid Sinus Baroreflex in Rats with Vascular Calcification |
title_full_unstemmed | Hydrogen Sulfide Facilitates the Impaired Sensitivity of Carotid Sinus Baroreflex in Rats with Vascular Calcification |
title_short | Hydrogen Sulfide Facilitates the Impaired Sensitivity of Carotid Sinus Baroreflex in Rats with Vascular Calcification |
title_sort | hydrogen sulfide facilitates the impaired sensitivity of carotid sinus baroreflex in rats with vascular calcification |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5601057/ https://www.ncbi.nlm.nih.gov/pubmed/28955233 http://dx.doi.org/10.3389/fphar.2017.00629 |
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