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Exosome-encapsulated microRNAs as circulating biomarkers for colorectal cancer

Currently available studies have suggested that a number of exosome-encapsulated microRNAs (miRNAs) are recognized as stable biomarkers for cancers. However, little is known about the effect of exosomal miRNAs on colorectal cancer (CRC). The aim of study is to identify specific miRNAs in serum exoso...

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Autores principales: Yan, Shushan, Han, Bing, Gao, Shunyuan, Wang, Xiaochen, Wang, Zengfang, Wang, Fakai, Zhang, Jianjun, Xu, Donghua, Sun, Beicheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5601128/
https://www.ncbi.nlm.nih.gov/pubmed/28947960
http://dx.doi.org/10.18632/oncotarget.18557
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author Yan, Shushan
Han, Bing
Gao, Shunyuan
Wang, Xiaochen
Wang, Zengfang
Wang, Fakai
Zhang, Jianjun
Xu, Donghua
Sun, Beicheng
author_facet Yan, Shushan
Han, Bing
Gao, Shunyuan
Wang, Xiaochen
Wang, Zengfang
Wang, Fakai
Zhang, Jianjun
Xu, Donghua
Sun, Beicheng
author_sort Yan, Shushan
collection PubMed
description Currently available studies have suggested that a number of exosome-encapsulated microRNAs (miRNAs) are recognized as stable biomarkers for cancers. However, little is known about the effect of exosomal miRNAs on colorectal cancer (CRC). The aim of study is to identify specific miRNAs in serum exosomes, which may serve as potential diagnostic and prognostic biomarkers and therapeutic targets for CRC. Microarray analyses of miRNAs in serum exosomes from 3 primary CRC patients and 3 healthy controls were performed. Those differentially expressed exosome-encapsulated miRNAs were verified in exosome-enriched serum samples from 77 CRC patients and 20 healthy controls by quantitative real-time PCR (qRT-PCR). A total of 39 aberrantly expressed miRNAs in serum exosomes were identified by microarray analysis. After confirmation by qRT-PCR, we found that 5 exosome-encapsulated miRNAs (miR-638, miR-5787, miR-8075, miR-6869-5p and miR-548c-5p) were significantly down-regulated, while 2 exosome-encapsulated miRNAs (miR-486-5p and miR-3180-5p) were significantly up-regulated in serum. Decreased levels of miR-638 in serum exosomes were associated with increased risk of liver metastasis and later TNM stage of CRC. Networks analyses revealed that 5 aberrantly expressed miRNAs (miR-638, miR-5787, miR-8075, miR-6869-5p, and miR-548c-5p) might be involved in the process of glucose metabolism in CRC. The present study shows the specific serum profile of exosome-encapsulated miRNAs in CRC. Those specific miRNAs in serum exosomes may serve as disease biomarkers and novel therapeutic targets for CRC.
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spelling pubmed-56011282017-09-25 Exosome-encapsulated microRNAs as circulating biomarkers for colorectal cancer Yan, Shushan Han, Bing Gao, Shunyuan Wang, Xiaochen Wang, Zengfang Wang, Fakai Zhang, Jianjun Xu, Donghua Sun, Beicheng Oncotarget Research Paper Currently available studies have suggested that a number of exosome-encapsulated microRNAs (miRNAs) are recognized as stable biomarkers for cancers. However, little is known about the effect of exosomal miRNAs on colorectal cancer (CRC). The aim of study is to identify specific miRNAs in serum exosomes, which may serve as potential diagnostic and prognostic biomarkers and therapeutic targets for CRC. Microarray analyses of miRNAs in serum exosomes from 3 primary CRC patients and 3 healthy controls were performed. Those differentially expressed exosome-encapsulated miRNAs were verified in exosome-enriched serum samples from 77 CRC patients and 20 healthy controls by quantitative real-time PCR (qRT-PCR). A total of 39 aberrantly expressed miRNAs in serum exosomes were identified by microarray analysis. After confirmation by qRT-PCR, we found that 5 exosome-encapsulated miRNAs (miR-638, miR-5787, miR-8075, miR-6869-5p and miR-548c-5p) were significantly down-regulated, while 2 exosome-encapsulated miRNAs (miR-486-5p and miR-3180-5p) were significantly up-regulated in serum. Decreased levels of miR-638 in serum exosomes were associated with increased risk of liver metastasis and later TNM stage of CRC. Networks analyses revealed that 5 aberrantly expressed miRNAs (miR-638, miR-5787, miR-8075, miR-6869-5p, and miR-548c-5p) might be involved in the process of glucose metabolism in CRC. The present study shows the specific serum profile of exosome-encapsulated miRNAs in CRC. Those specific miRNAs in serum exosomes may serve as disease biomarkers and novel therapeutic targets for CRC. Impact Journals LLC 2017-06-16 /pmc/articles/PMC5601128/ /pubmed/28947960 http://dx.doi.org/10.18632/oncotarget.18557 Text en Copyright: © 2017 Yan et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Paper
Yan, Shushan
Han, Bing
Gao, Shunyuan
Wang, Xiaochen
Wang, Zengfang
Wang, Fakai
Zhang, Jianjun
Xu, Donghua
Sun, Beicheng
Exosome-encapsulated microRNAs as circulating biomarkers for colorectal cancer
title Exosome-encapsulated microRNAs as circulating biomarkers for colorectal cancer
title_full Exosome-encapsulated microRNAs as circulating biomarkers for colorectal cancer
title_fullStr Exosome-encapsulated microRNAs as circulating biomarkers for colorectal cancer
title_full_unstemmed Exosome-encapsulated microRNAs as circulating biomarkers for colorectal cancer
title_short Exosome-encapsulated microRNAs as circulating biomarkers for colorectal cancer
title_sort exosome-encapsulated micrornas as circulating biomarkers for colorectal cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5601128/
https://www.ncbi.nlm.nih.gov/pubmed/28947960
http://dx.doi.org/10.18632/oncotarget.18557
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