Cargando…

Inhibition of avian tumor virus replication by CCCH-type zinc finger antiviral protein

CCCH type zinc finger antiviral protein (ZAP) is a host restriction factor that inhibits the replication of a variety of viruses in mammals. However, little is known about its antiviral activity on avian tumor virus. Avian leukosis virus subgroup J (ALV-J), an oncogenic retrovirus, induces myelocyto...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, Mingjun, Ma, Xiaoqian, Cui, Xiyao, Zhou, Jing, Li, Chengui, Huang, Libo, Shang, Yingli, Cheng, Ziqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5601699/
https://www.ncbi.nlm.nih.gov/pubmed/28938603
http://dx.doi.org/10.18632/oncotarget.19378
_version_ 1783264436303167488
author Zhu, Mingjun
Ma, Xiaoqian
Cui, Xiyao
Zhou, Jing
Li, Chengui
Huang, Libo
Shang, Yingli
Cheng, Ziqiang
author_facet Zhu, Mingjun
Ma, Xiaoqian
Cui, Xiyao
Zhou, Jing
Li, Chengui
Huang, Libo
Shang, Yingli
Cheng, Ziqiang
author_sort Zhu, Mingjun
collection PubMed
description CCCH type zinc finger antiviral protein (ZAP) is a host restriction factor that inhibits the replication of a variety of viruses in mammals. However, little is known about its antiviral activity on avian tumor virus. Avian leukosis virus subgroup J (ALV-J), an oncogenic retrovirus, induces myelocytomas and various other tumors in meat and egg type chickens. Here, we identified a chicken ZAP (chZAP) that increased at early stage, and subsequently decreased after infection of ALV-J in DF-1 cells, indicating the inducible feature of the endogenous chZAP. To demonstrate the inhibitory effect on ALV-J replication by chZAP, we expressed exogenous chZAP by lentivirus based vectors in DF-1 cells that infected by ALV-J. The result showed that overexpression of chZAP significantly inhibited ALV-J replication at both mRNA level and protein level. Consequently, knockdown of endogenous chZAP by RNAi facilitated ALV-J replication in DF-1 cells. Further, we demonstrated that chZAP interacts with SU protein (encode by gp85 gene) of ALV-J in cytoplasm. Taken together, our results demonstrated that chZAP inhibits ALV-J by both mRNA and protein pathway and it may shed light on a novel antiviral approach in poultry.
format Online
Article
Text
id pubmed-5601699
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Impact Journals LLC
record_format MEDLINE/PubMed
spelling pubmed-56016992017-09-21 Inhibition of avian tumor virus replication by CCCH-type zinc finger antiviral protein Zhu, Mingjun Ma, Xiaoqian Cui, Xiyao Zhou, Jing Li, Chengui Huang, Libo Shang, Yingli Cheng, Ziqiang Oncotarget Research Paper CCCH type zinc finger antiviral protein (ZAP) is a host restriction factor that inhibits the replication of a variety of viruses in mammals. However, little is known about its antiviral activity on avian tumor virus. Avian leukosis virus subgroup J (ALV-J), an oncogenic retrovirus, induces myelocytomas and various other tumors in meat and egg type chickens. Here, we identified a chicken ZAP (chZAP) that increased at early stage, and subsequently decreased after infection of ALV-J in DF-1 cells, indicating the inducible feature of the endogenous chZAP. To demonstrate the inhibitory effect on ALV-J replication by chZAP, we expressed exogenous chZAP by lentivirus based vectors in DF-1 cells that infected by ALV-J. The result showed that overexpression of chZAP significantly inhibited ALV-J replication at both mRNA level and protein level. Consequently, knockdown of endogenous chZAP by RNAi facilitated ALV-J replication in DF-1 cells. Further, we demonstrated that chZAP interacts with SU protein (encode by gp85 gene) of ALV-J in cytoplasm. Taken together, our results demonstrated that chZAP inhibits ALV-J by both mRNA and protein pathway and it may shed light on a novel antiviral approach in poultry. Impact Journals LLC 2017-07-19 /pmc/articles/PMC5601699/ /pubmed/28938603 http://dx.doi.org/10.18632/oncotarget.19378 Text en Copyright: © 2017 Zhu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Zhu, Mingjun
Ma, Xiaoqian
Cui, Xiyao
Zhou, Jing
Li, Chengui
Huang, Libo
Shang, Yingli
Cheng, Ziqiang
Inhibition of avian tumor virus replication by CCCH-type zinc finger antiviral protein
title Inhibition of avian tumor virus replication by CCCH-type zinc finger antiviral protein
title_full Inhibition of avian tumor virus replication by CCCH-type zinc finger antiviral protein
title_fullStr Inhibition of avian tumor virus replication by CCCH-type zinc finger antiviral protein
title_full_unstemmed Inhibition of avian tumor virus replication by CCCH-type zinc finger antiviral protein
title_short Inhibition of avian tumor virus replication by CCCH-type zinc finger antiviral protein
title_sort inhibition of avian tumor virus replication by ccch-type zinc finger antiviral protein
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5601699/
https://www.ncbi.nlm.nih.gov/pubmed/28938603
http://dx.doi.org/10.18632/oncotarget.19378
work_keys_str_mv AT zhumingjun inhibitionofaviantumorvirusreplicationbyccchtypezincfingerantiviralprotein
AT maxiaoqian inhibitionofaviantumorvirusreplicationbyccchtypezincfingerantiviralprotein
AT cuixiyao inhibitionofaviantumorvirusreplicationbyccchtypezincfingerantiviralprotein
AT zhoujing inhibitionofaviantumorvirusreplicationbyccchtypezincfingerantiviralprotein
AT lichengui inhibitionofaviantumorvirusreplicationbyccchtypezincfingerantiviralprotein
AT huanglibo inhibitionofaviantumorvirusreplicationbyccchtypezincfingerantiviralprotein
AT shangyingli inhibitionofaviantumorvirusreplicationbyccchtypezincfingerantiviralprotein
AT chengziqiang inhibitionofaviantumorvirusreplicationbyccchtypezincfingerantiviralprotein