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miR-543 promotes colorectal cancer proliferation and metastasis by targeting KLF4
Till now, miR-543 expression has been demonstrated to be involved in the development of some cancers. However, reports about its expression and mechanism in colorectal cancer (CRC) were conflicting [1, 2]. Here, we investigated clinical implications of miR-543 and mechanisms underlying miR-543-media...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5601729/ https://www.ncbi.nlm.nih.gov/pubmed/28938633 http://dx.doi.org/10.18632/oncotarget.19495 |
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author | Zhai, Fangbing Cao, Chunhong Zhang, Liang Zhang, Jianhua |
author_facet | Zhai, Fangbing Cao, Chunhong Zhang, Liang Zhang, Jianhua |
author_sort | Zhai, Fangbing |
collection | PubMed |
description | Till now, miR-543 expression has been demonstrated to be involved in the development of some cancers. However, reports about its expression and mechanism in colorectal cancer (CRC) were conflicting [1, 2]. Here, we investigated clinical implications of miR-543 and mechanisms underlying miR-543-mediated CRC development. In this study, real-time quantitative PCR (qRT-PCR) validated miR-543 was highly expressed in CRC samples and cell lines. MiR-543 was closely associated with tumor size, TNM stage and metastasis. In addition, survival analysis showed that high miR-543 expression was obviously correlated with poor overall survival and disease-free survival. Mechanically, downregulation of miR-543 by miR-543 inhibitor obviously repressed cell proliferation, promoted apoptosis, affected migration and invasion. Moreover, luciferase reporter analysis identified that Krüppel-like Factor-4 (KLF4) was a direct target of miR-543, and there was an obvious inverse correlation between miR-543 and KLF4 expression in CRC tissues. Furthermore, KLF4 down-regulation favors miR-543-induced oncogenic effect on cell proliferation, apoptosis, migration, and invasion. In conclusion, this study indicated that miR-543 facilitates colorectal cancer proliferation and metastasis by targeting KLF4, and miR-543 may serve as a promising target for the treatment of CRC patients. |
format | Online Article Text |
id | pubmed-5601729 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-56017292017-09-21 miR-543 promotes colorectal cancer proliferation and metastasis by targeting KLF4 Zhai, Fangbing Cao, Chunhong Zhang, Liang Zhang, Jianhua Oncotarget Research Paper Till now, miR-543 expression has been demonstrated to be involved in the development of some cancers. However, reports about its expression and mechanism in colorectal cancer (CRC) were conflicting [1, 2]. Here, we investigated clinical implications of miR-543 and mechanisms underlying miR-543-mediated CRC development. In this study, real-time quantitative PCR (qRT-PCR) validated miR-543 was highly expressed in CRC samples and cell lines. MiR-543 was closely associated with tumor size, TNM stage and metastasis. In addition, survival analysis showed that high miR-543 expression was obviously correlated with poor overall survival and disease-free survival. Mechanically, downregulation of miR-543 by miR-543 inhibitor obviously repressed cell proliferation, promoted apoptosis, affected migration and invasion. Moreover, luciferase reporter analysis identified that Krüppel-like Factor-4 (KLF4) was a direct target of miR-543, and there was an obvious inverse correlation between miR-543 and KLF4 expression in CRC tissues. Furthermore, KLF4 down-regulation favors miR-543-induced oncogenic effect on cell proliferation, apoptosis, migration, and invasion. In conclusion, this study indicated that miR-543 facilitates colorectal cancer proliferation and metastasis by targeting KLF4, and miR-543 may serve as a promising target for the treatment of CRC patients. Impact Journals LLC 2017-07-22 /pmc/articles/PMC5601729/ /pubmed/28938633 http://dx.doi.org/10.18632/oncotarget.19495 Text en Copyright: © 2017 Zhai et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Zhai, Fangbing Cao, Chunhong Zhang, Liang Zhang, Jianhua miR-543 promotes colorectal cancer proliferation and metastasis by targeting KLF4 |
title | miR-543 promotes colorectal cancer proliferation and metastasis by targeting KLF4 |
title_full | miR-543 promotes colorectal cancer proliferation and metastasis by targeting KLF4 |
title_fullStr | miR-543 promotes colorectal cancer proliferation and metastasis by targeting KLF4 |
title_full_unstemmed | miR-543 promotes colorectal cancer proliferation and metastasis by targeting KLF4 |
title_short | miR-543 promotes colorectal cancer proliferation and metastasis by targeting KLF4 |
title_sort | mir-543 promotes colorectal cancer proliferation and metastasis by targeting klf4 |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5601729/ https://www.ncbi.nlm.nih.gov/pubmed/28938633 http://dx.doi.org/10.18632/oncotarget.19495 |
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