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A controlled comparison of the BacT/ALERT® 3D and VIRTUO™ microbial detection systems

The performance of the next-generation BacT/ALERT® VIRTUO™ Microbial Detection System (VIRTUO™, bioMérieux Inc., Hazelwood, MO) was compared to the BacT/ALERT® 3D Microbial Detection System (3D, bioMérieux Inc., Durham, NC) using BacT/ALERT® FA Plus (FA Plus), BacT/ALERT® PF Plus (PF Plus), BacT/ALE...

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Detalles Bibliográficos
Autores principales: Totty, H., Ullery, M., Spontak, J., Viray, J., Adamik, M., Katzin, B., Dunne, W. M., Deol, P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602088/
https://www.ncbi.nlm.nih.gov/pubmed/28500506
http://dx.doi.org/10.1007/s10096-017-2994-8
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author Totty, H.
Ullery, M.
Spontak, J.
Viray, J.
Adamik, M.
Katzin, B.
Dunne, W. M.
Deol, P.
author_facet Totty, H.
Ullery, M.
Spontak, J.
Viray, J.
Adamik, M.
Katzin, B.
Dunne, W. M.
Deol, P.
author_sort Totty, H.
collection PubMed
description The performance of the next-generation BacT/ALERT® VIRTUO™ Microbial Detection System (VIRTUO™, bioMérieux Inc., Hazelwood, MO) was compared to the BacT/ALERT® 3D Microbial Detection System (3D, bioMérieux Inc., Durham, NC) using BacT/ALERT® FA Plus (FA Plus), BacT/ALERT® PF Plus (PF Plus), BacT/ALERT® FN Plus (FN Plus), BacT/ALERT® Standard Aerobic (SA), and BacT/ALERT® Standard Anaerobic (SN) blood culture bottles (bioMérieux Inc., Durham, NC). A seeded limit of detection (LoD) study was performed for each bottle type in both systems. The LoD studies demonstrated that both systems were capable of detecting organisms at nearly identical levels [<10 colony-forming units (CFU) per bottle], with no significant difference. Following LoD determination, a seeded study was performed to compare the time to detection (TTD) between the systems using a panel of clinically relevant microorganisms inoculated at or near the LoD with 0, 4, or 10 mL of healthy human blood. VIRTUO™ exhibited a faster TTD by an average of 3.5 h, as well as demonstrated a significantly improved detection rate of 99.9% compared to 98.8% with 3D (p-value <0.05). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10096-017-2994-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-56020882017-10-04 A controlled comparison of the BacT/ALERT® 3D and VIRTUO™ microbial detection systems Totty, H. Ullery, M. Spontak, J. Viray, J. Adamik, M. Katzin, B. Dunne, W. M. Deol, P. Eur J Clin Microbiol Infect Dis Original Article The performance of the next-generation BacT/ALERT® VIRTUO™ Microbial Detection System (VIRTUO™, bioMérieux Inc., Hazelwood, MO) was compared to the BacT/ALERT® 3D Microbial Detection System (3D, bioMérieux Inc., Durham, NC) using BacT/ALERT® FA Plus (FA Plus), BacT/ALERT® PF Plus (PF Plus), BacT/ALERT® FN Plus (FN Plus), BacT/ALERT® Standard Aerobic (SA), and BacT/ALERT® Standard Anaerobic (SN) blood culture bottles (bioMérieux Inc., Durham, NC). A seeded limit of detection (LoD) study was performed for each bottle type in both systems. The LoD studies demonstrated that both systems were capable of detecting organisms at nearly identical levels [<10 colony-forming units (CFU) per bottle], with no significant difference. Following LoD determination, a seeded study was performed to compare the time to detection (TTD) between the systems using a panel of clinically relevant microorganisms inoculated at or near the LoD with 0, 4, or 10 mL of healthy human blood. VIRTUO™ exhibited a faster TTD by an average of 3.5 h, as well as demonstrated a significantly improved detection rate of 99.9% compared to 98.8% with 3D (p-value <0.05). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10096-017-2994-8) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2017-05-12 2017 /pmc/articles/PMC5602088/ /pubmed/28500506 http://dx.doi.org/10.1007/s10096-017-2994-8 Text en © The Author(s) 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Totty, H.
Ullery, M.
Spontak, J.
Viray, J.
Adamik, M.
Katzin, B.
Dunne, W. M.
Deol, P.
A controlled comparison of the BacT/ALERT® 3D and VIRTUO™ microbial detection systems
title A controlled comparison of the BacT/ALERT® 3D and VIRTUO™ microbial detection systems
title_full A controlled comparison of the BacT/ALERT® 3D and VIRTUO™ microbial detection systems
title_fullStr A controlled comparison of the BacT/ALERT® 3D and VIRTUO™ microbial detection systems
title_full_unstemmed A controlled comparison of the BacT/ALERT® 3D and VIRTUO™ microbial detection systems
title_short A controlled comparison of the BacT/ALERT® 3D and VIRTUO™ microbial detection systems
title_sort controlled comparison of the bact/alert® 3d and virtuo™ microbial detection systems
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602088/
https://www.ncbi.nlm.nih.gov/pubmed/28500506
http://dx.doi.org/10.1007/s10096-017-2994-8
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