Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals

To understand the epidemiological significance of Pneumocystis detection in a lung tissue sample of non-immunosuppressed individuals, we examined sampling procedures, laboratory methodology, and patient characteristics of autopsy series reported in the literature. Number of tissue specimens, DNA-ext...

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Autores principales: Vargas, S. L., Ponce, C., Bustamante, R., Calderón, E., Nevez, G., De Armas, Y., Matos, O., Miller, R. F., Gallo, M. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602097/
https://www.ncbi.nlm.nih.gov/pubmed/28584896
http://dx.doi.org/10.1007/s10096-017-3006-8
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author Vargas, S. L.
Ponce, C.
Bustamante, R.
Calderón, E.
Nevez, G.
De Armas, Y.
Matos, O.
Miller, R. F.
Gallo, M. J.
author_facet Vargas, S. L.
Ponce, C.
Bustamante, R.
Calderón, E.
Nevez, G.
De Armas, Y.
Matos, O.
Miller, R. F.
Gallo, M. J.
author_sort Vargas, S. L.
collection PubMed
description To understand the epidemiological significance of Pneumocystis detection in a lung tissue sample of non-immunosuppressed individuals, we examined sampling procedures, laboratory methodology, and patient characteristics of autopsy series reported in the literature. Number of tissue specimens, DNA-extraction procedures, age and underlying diagnosis highly influence yield and are critical to understand yield differences of Pneumocystis among reports of pulmonary colonization in immunocompetent individuals.
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spelling pubmed-56020972017-10-04 Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals Vargas, S. L. Ponce, C. Bustamante, R. Calderón, E. Nevez, G. De Armas, Y. Matos, O. Miller, R. F. Gallo, M. J. Eur J Clin Microbiol Infect Dis Review To understand the epidemiological significance of Pneumocystis detection in a lung tissue sample of non-immunosuppressed individuals, we examined sampling procedures, laboratory methodology, and patient characteristics of autopsy series reported in the literature. Number of tissue specimens, DNA-extraction procedures, age and underlying diagnosis highly influence yield and are critical to understand yield differences of Pneumocystis among reports of pulmonary colonization in immunocompetent individuals. Springer Berlin Heidelberg 2017-06-05 2017 /pmc/articles/PMC5602097/ /pubmed/28584896 http://dx.doi.org/10.1007/s10096-017-3006-8 Text en © The Author(s) 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Review
Vargas, S. L.
Ponce, C.
Bustamante, R.
Calderón, E.
Nevez, G.
De Armas, Y.
Matos, O.
Miller, R. F.
Gallo, M. J.
Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals
title Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals
title_full Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals
title_fullStr Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals
title_full_unstemmed Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals
title_short Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals
title_sort importance of tissue sampling, laboratory methods, and patient characteristics for detection of pneumocystis in autopsied lungs of non-immunosuppressed individuals
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602097/
https://www.ncbi.nlm.nih.gov/pubmed/28584896
http://dx.doi.org/10.1007/s10096-017-3006-8
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