Cargando…
Enzymatic production of single-molecule FISH and RNA capture probes
Arrays of singly labeled short oligonucleotides that hybridize to a specific target revolutionized RNA biology, enabling quantitative, single-molecule microscopy analysis and high-efficiency RNA/RNP capture. Here, we describe a simple and efficient method that allows flexible functionalization of in...
Autores principales: | Gaspar, Imre, Wippich, Frank, Ephrussi, Anne |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602115/ https://www.ncbi.nlm.nih.gov/pubmed/28698239 http://dx.doi.org/10.1261/rna.061184.117 |
Ejemplares similares
-
Strength in numbers: quantitative single‐molecule RNA detection assays
por: Gaspar, Imre, et al.
Publicado: (2015) -
Nutritional stress-induced regulation of microtubule organization and mRNP transport by HDAC1 controlled α-tubulin acetylation
por: Wippich, Frank, et al.
Publicado: (2023) -
Multiplex target capture with double-stranded DNA probes
por: Shen, Peidong, et al.
Publicado: (2013) -
Nondestructive enzymatic deamination enables single-molecule long-read amplicon sequencing for the determination of 5-methylcytosine and 5-hydroxymethylcytosine at single-base resolution
por: Sun, Zhiyi, et al.
Publicado: (2021) -
Single molecule molecular inversion probes for targeted, high-accuracy detection of low-frequency variation
por: Hiatt, Joseph B., et al.
Publicado: (2013)