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miR-509-5p inhibits cellular proliferation and migration via targeting MDM2 in pancreatic cancer cells
OBJECTIVE: This study aimed to explore the effect of miR-509-5p on pancreatic cancer progression and clarify the underlying mechanisms. METHODS: Real-time quantitative reverse transcription polymerase chain reaction was employed to determine miR-509-5p expression in pancreatic cancer tissues and non...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602284/ https://www.ncbi.nlm.nih.gov/pubmed/28979137 http://dx.doi.org/10.2147/OTT.S130378 |
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author | Li, Xin Li, Yan Wan, Li Chen, Rui Chen, Fei |
author_facet | Li, Xin Li, Yan Wan, Li Chen, Rui Chen, Fei |
author_sort | Li, Xin |
collection | PubMed |
description | OBJECTIVE: This study aimed to explore the effect of miR-509-5p on pancreatic cancer progression and clarify the underlying mechanisms. METHODS: Real-time quantitative reverse transcription polymerase chain reaction was employed to determine miR-509-5p expression in pancreatic cancer tissues and noncancerous adjacent tissues. CCK-8 and Transwell experiments were employed to examine cellular proliferation, migration, and invasion after miR-509-5p mimic or inhibitor transfection. Bioinformatics tools were used to identify the target gene of miR-509-5p, and cotransfection of the target gene and miR-509-5p mimic was performed to determine the effect on the proliferation and migration of pancreatic cancer cells. A xenograft mouse model and histological analysis were also used to test the effect of miR-509-5p on tumor growth in vivo. RESULTS: miR-509-5p expression was dramatically downregulated in pancreatic cancer tissues and in pancreatic cancer cell lines. miR-509-5p mimic markedly inhibited PANC-1 cell proliferation, migration, and invasion. Conversely, miR-509-5p inhibitor promoted PANC-1 cell proliferation, migration, and invasion. Furthermore, the 3′UTR–specific target site luciferase reporter assay also showed that miR-509-5p negatively regulated MDM2 at the post-transcriptional level. miR-509-5p effectively reversed the MDM2 overexpression-induced increase in PANC-1 cell proliferation and invasion. Moreover, miR-509-5p inhibited tumor growth and accelerated cell death in the tumor samples. CONCLUSIONS: Our results suggested that miR-509-5p served as a new tumor suppressor via targeting the MDM2 gene, inhibiting pancreatic cancer progression. |
format | Online Article Text |
id | pubmed-5602284 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-56022842017-10-04 miR-509-5p inhibits cellular proliferation and migration via targeting MDM2 in pancreatic cancer cells Li, Xin Li, Yan Wan, Li Chen, Rui Chen, Fei Onco Targets Ther Original Research OBJECTIVE: This study aimed to explore the effect of miR-509-5p on pancreatic cancer progression and clarify the underlying mechanisms. METHODS: Real-time quantitative reverse transcription polymerase chain reaction was employed to determine miR-509-5p expression in pancreatic cancer tissues and noncancerous adjacent tissues. CCK-8 and Transwell experiments were employed to examine cellular proliferation, migration, and invasion after miR-509-5p mimic or inhibitor transfection. Bioinformatics tools were used to identify the target gene of miR-509-5p, and cotransfection of the target gene and miR-509-5p mimic was performed to determine the effect on the proliferation and migration of pancreatic cancer cells. A xenograft mouse model and histological analysis were also used to test the effect of miR-509-5p on tumor growth in vivo. RESULTS: miR-509-5p expression was dramatically downregulated in pancreatic cancer tissues and in pancreatic cancer cell lines. miR-509-5p mimic markedly inhibited PANC-1 cell proliferation, migration, and invasion. Conversely, miR-509-5p inhibitor promoted PANC-1 cell proliferation, migration, and invasion. Furthermore, the 3′UTR–specific target site luciferase reporter assay also showed that miR-509-5p negatively regulated MDM2 at the post-transcriptional level. miR-509-5p effectively reversed the MDM2 overexpression-induced increase in PANC-1 cell proliferation and invasion. Moreover, miR-509-5p inhibited tumor growth and accelerated cell death in the tumor samples. CONCLUSIONS: Our results suggested that miR-509-5p served as a new tumor suppressor via targeting the MDM2 gene, inhibiting pancreatic cancer progression. Dove Medical Press 2017-09-11 /pmc/articles/PMC5602284/ /pubmed/28979137 http://dx.doi.org/10.2147/OTT.S130378 Text en © 2017 Li et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Li, Xin Li, Yan Wan, Li Chen, Rui Chen, Fei miR-509-5p inhibits cellular proliferation and migration via targeting MDM2 in pancreatic cancer cells |
title | miR-509-5p inhibits cellular proliferation and migration via targeting MDM2 in pancreatic cancer cells |
title_full | miR-509-5p inhibits cellular proliferation and migration via targeting MDM2 in pancreatic cancer cells |
title_fullStr | miR-509-5p inhibits cellular proliferation and migration via targeting MDM2 in pancreatic cancer cells |
title_full_unstemmed | miR-509-5p inhibits cellular proliferation and migration via targeting MDM2 in pancreatic cancer cells |
title_short | miR-509-5p inhibits cellular proliferation and migration via targeting MDM2 in pancreatic cancer cells |
title_sort | mir-509-5p inhibits cellular proliferation and migration via targeting mdm2 in pancreatic cancer cells |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602284/ https://www.ncbi.nlm.nih.gov/pubmed/28979137 http://dx.doi.org/10.2147/OTT.S130378 |
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