Cargando…

miR-509-5p inhibits cellular proliferation and migration via targeting MDM2 in pancreatic cancer cells

OBJECTIVE: This study aimed to explore the effect of miR-509-5p on pancreatic cancer progression and clarify the underlying mechanisms. METHODS: Real-time quantitative reverse transcription polymerase chain reaction was employed to determine miR-509-5p expression in pancreatic cancer tissues and non...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Xin, Li, Yan, Wan, Li, Chen, Rui, Chen, Fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602284/
https://www.ncbi.nlm.nih.gov/pubmed/28979137
http://dx.doi.org/10.2147/OTT.S130378
_version_ 1783264553292791808
author Li, Xin
Li, Yan
Wan, Li
Chen, Rui
Chen, Fei
author_facet Li, Xin
Li, Yan
Wan, Li
Chen, Rui
Chen, Fei
author_sort Li, Xin
collection PubMed
description OBJECTIVE: This study aimed to explore the effect of miR-509-5p on pancreatic cancer progression and clarify the underlying mechanisms. METHODS: Real-time quantitative reverse transcription polymerase chain reaction was employed to determine miR-509-5p expression in pancreatic cancer tissues and noncancerous adjacent tissues. CCK-8 and Transwell experiments were employed to examine cellular proliferation, migration, and invasion after miR-509-5p mimic or inhibitor transfection. Bioinformatics tools were used to identify the target gene of miR-509-5p, and cotransfection of the target gene and miR-509-5p mimic was performed to determine the effect on the proliferation and migration of pancreatic cancer cells. A xenograft mouse model and histological analysis were also used to test the effect of miR-509-5p on tumor growth in vivo. RESULTS: miR-509-5p expression was dramatically downregulated in pancreatic cancer tissues and in pancreatic cancer cell lines. miR-509-5p mimic markedly inhibited PANC-1 cell proliferation, migration, and invasion. Conversely, miR-509-5p inhibitor promoted PANC-1 cell proliferation, migration, and invasion. Furthermore, the 3′UTR–specific target site luciferase reporter assay also showed that miR-509-5p negatively regulated MDM2 at the post-transcriptional level. miR-509-5p effectively reversed the MDM2 overexpression-induced increase in PANC-1 cell proliferation and invasion. Moreover, miR-509-5p inhibited tumor growth and accelerated cell death in the tumor samples. CONCLUSIONS: Our results suggested that miR-509-5p served as a new tumor suppressor via targeting the MDM2 gene, inhibiting pancreatic cancer progression.
format Online
Article
Text
id pubmed-5602284
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Dove Medical Press
record_format MEDLINE/PubMed
spelling pubmed-56022842017-10-04 miR-509-5p inhibits cellular proliferation and migration via targeting MDM2 in pancreatic cancer cells Li, Xin Li, Yan Wan, Li Chen, Rui Chen, Fei Onco Targets Ther Original Research OBJECTIVE: This study aimed to explore the effect of miR-509-5p on pancreatic cancer progression and clarify the underlying mechanisms. METHODS: Real-time quantitative reverse transcription polymerase chain reaction was employed to determine miR-509-5p expression in pancreatic cancer tissues and noncancerous adjacent tissues. CCK-8 and Transwell experiments were employed to examine cellular proliferation, migration, and invasion after miR-509-5p mimic or inhibitor transfection. Bioinformatics tools were used to identify the target gene of miR-509-5p, and cotransfection of the target gene and miR-509-5p mimic was performed to determine the effect on the proliferation and migration of pancreatic cancer cells. A xenograft mouse model and histological analysis were also used to test the effect of miR-509-5p on tumor growth in vivo. RESULTS: miR-509-5p expression was dramatically downregulated in pancreatic cancer tissues and in pancreatic cancer cell lines. miR-509-5p mimic markedly inhibited PANC-1 cell proliferation, migration, and invasion. Conversely, miR-509-5p inhibitor promoted PANC-1 cell proliferation, migration, and invasion. Furthermore, the 3′UTR–specific target site luciferase reporter assay also showed that miR-509-5p negatively regulated MDM2 at the post-transcriptional level. miR-509-5p effectively reversed the MDM2 overexpression-induced increase in PANC-1 cell proliferation and invasion. Moreover, miR-509-5p inhibited tumor growth and accelerated cell death in the tumor samples. CONCLUSIONS: Our results suggested that miR-509-5p served as a new tumor suppressor via targeting the MDM2 gene, inhibiting pancreatic cancer progression. Dove Medical Press 2017-09-11 /pmc/articles/PMC5602284/ /pubmed/28979137 http://dx.doi.org/10.2147/OTT.S130378 Text en © 2017 Li et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Li, Xin
Li, Yan
Wan, Li
Chen, Rui
Chen, Fei
miR-509-5p inhibits cellular proliferation and migration via targeting MDM2 in pancreatic cancer cells
title miR-509-5p inhibits cellular proliferation and migration via targeting MDM2 in pancreatic cancer cells
title_full miR-509-5p inhibits cellular proliferation and migration via targeting MDM2 in pancreatic cancer cells
title_fullStr miR-509-5p inhibits cellular proliferation and migration via targeting MDM2 in pancreatic cancer cells
title_full_unstemmed miR-509-5p inhibits cellular proliferation and migration via targeting MDM2 in pancreatic cancer cells
title_short miR-509-5p inhibits cellular proliferation and migration via targeting MDM2 in pancreatic cancer cells
title_sort mir-509-5p inhibits cellular proliferation and migration via targeting mdm2 in pancreatic cancer cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602284/
https://www.ncbi.nlm.nih.gov/pubmed/28979137
http://dx.doi.org/10.2147/OTT.S130378
work_keys_str_mv AT lixin mir5095pinhibitscellularproliferationandmigrationviatargetingmdm2inpancreaticcancercells
AT liyan mir5095pinhibitscellularproliferationandmigrationviatargetingmdm2inpancreaticcancercells
AT wanli mir5095pinhibitscellularproliferationandmigrationviatargetingmdm2inpancreaticcancercells
AT chenrui mir5095pinhibitscellularproliferationandmigrationviatargetingmdm2inpancreaticcancercells
AT chenfei mir5095pinhibitscellularproliferationandmigrationviatargetingmdm2inpancreaticcancercells