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Transcriptional correlates of memory maintenance following long-term sensitization of Aplysia californica

We characterized the transcriptional response accompanying maintenance of long-term sensitization (LTS) memory in the pleural ganglia of Aplysia californica using microarray (N = 8) and qPCR (N = 11 additional samples). We found that 24 h after memory induction there is strong regulation of 1198 tra...

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Autores principales: Conte, Catherine, Herdegen, Samantha, Kamal, Saman, Patel, Jency, Patel, Ushma, Perez, Leticia, Rivota, Marissa, Calin-Jageman, Robert J., Calin-Jageman, Irina E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602346/
https://www.ncbi.nlm.nih.gov/pubmed/28916625
http://dx.doi.org/10.1101/lm.045450.117
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author Conte, Catherine
Herdegen, Samantha
Kamal, Saman
Patel, Jency
Patel, Ushma
Perez, Leticia
Rivota, Marissa
Calin-Jageman, Robert J.
Calin-Jageman, Irina E.
author_facet Conte, Catherine
Herdegen, Samantha
Kamal, Saman
Patel, Jency
Patel, Ushma
Perez, Leticia
Rivota, Marissa
Calin-Jageman, Robert J.
Calin-Jageman, Irina E.
author_sort Conte, Catherine
collection PubMed
description We characterized the transcriptional response accompanying maintenance of long-term sensitization (LTS) memory in the pleural ganglia of Aplysia californica using microarray (N = 8) and qPCR (N = 11 additional samples). We found that 24 h after memory induction there is strong regulation of 1198 transcripts (748 up and 450 down) in a pattern that is almost completely distinct from what is observed during memory encoding (1 h after training). There is widespread up-regulation of transcripts related to all levels of protein production, from transcription (e.g., subunits of transcription initiation factors) to translation (e.g., subunits of eIF1, eIF2, eIF3, eIF4, eIF5, and eIF2B) to activation of components of the unfolded protein response (e.g., CREB3/Luman, BiP, AATF). In addition, there are widespread changes in transcripts related to cytoskeleton function, synaptic targeting, synaptic function, neurotransmitter regulation, and neuronal signaling. Many of the transcripts identified have previously been linked to memory and plasticity (e.g., Egr, menin, TOB1, IGF2 mRNA binding protein 1/ZBP-1), though the majority are novel and/or uncharacterized. Interestingly, there is regulation that could contribute to metaplasticity potentially opposing or even eroding LTS memory (down-regulation of adenylate cyclase and a putative serotonin receptor, up-regulation of FMRFa and a FMRFa receptor). This study reveals that maintenance of a “simple” nonassociative memory is accompanied by an astonishingly complex transcriptional response.
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spelling pubmed-56023462018-10-01 Transcriptional correlates of memory maintenance following long-term sensitization of Aplysia californica Conte, Catherine Herdegen, Samantha Kamal, Saman Patel, Jency Patel, Ushma Perez, Leticia Rivota, Marissa Calin-Jageman, Robert J. Calin-Jageman, Irina E. Learn Mem Research We characterized the transcriptional response accompanying maintenance of long-term sensitization (LTS) memory in the pleural ganglia of Aplysia californica using microarray (N = 8) and qPCR (N = 11 additional samples). We found that 24 h after memory induction there is strong regulation of 1198 transcripts (748 up and 450 down) in a pattern that is almost completely distinct from what is observed during memory encoding (1 h after training). There is widespread up-regulation of transcripts related to all levels of protein production, from transcription (e.g., subunits of transcription initiation factors) to translation (e.g., subunits of eIF1, eIF2, eIF3, eIF4, eIF5, and eIF2B) to activation of components of the unfolded protein response (e.g., CREB3/Luman, BiP, AATF). In addition, there are widespread changes in transcripts related to cytoskeleton function, synaptic targeting, synaptic function, neurotransmitter regulation, and neuronal signaling. Many of the transcripts identified have previously been linked to memory and plasticity (e.g., Egr, menin, TOB1, IGF2 mRNA binding protein 1/ZBP-1), though the majority are novel and/or uncharacterized. Interestingly, there is regulation that could contribute to metaplasticity potentially opposing or even eroding LTS memory (down-regulation of adenylate cyclase and a putative serotonin receptor, up-regulation of FMRFa and a FMRFa receptor). This study reveals that maintenance of a “simple” nonassociative memory is accompanied by an astonishingly complex transcriptional response. Cold Spring Harbor Laboratory Press 2017-10 /pmc/articles/PMC5602346/ /pubmed/28916625 http://dx.doi.org/10.1101/lm.045450.117 Text en © 2017 Conte et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first 12 months after the full-issue publication date (see http://learnmem.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research
Conte, Catherine
Herdegen, Samantha
Kamal, Saman
Patel, Jency
Patel, Ushma
Perez, Leticia
Rivota, Marissa
Calin-Jageman, Robert J.
Calin-Jageman, Irina E.
Transcriptional correlates of memory maintenance following long-term sensitization of Aplysia californica
title Transcriptional correlates of memory maintenance following long-term sensitization of Aplysia californica
title_full Transcriptional correlates of memory maintenance following long-term sensitization of Aplysia californica
title_fullStr Transcriptional correlates of memory maintenance following long-term sensitization of Aplysia californica
title_full_unstemmed Transcriptional correlates of memory maintenance following long-term sensitization of Aplysia californica
title_short Transcriptional correlates of memory maintenance following long-term sensitization of Aplysia californica
title_sort transcriptional correlates of memory maintenance following long-term sensitization of aplysia californica
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602346/
https://www.ncbi.nlm.nih.gov/pubmed/28916625
http://dx.doi.org/10.1101/lm.045450.117
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