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Contextual fear conditioning in zebrafish
Zebrafish are a genetically tractable vertebrate that hold considerable promise for elucidating the molecular basis of behavior. Although numerous recent advances have been made in the ability to precisely manipulate the zebrafish genome, much less is known about many aspects of learning and memory...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602349/ https://www.ncbi.nlm.nih.gov/pubmed/28916626 http://dx.doi.org/10.1101/lm.045690.117 |
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author | Kenney, Justin W. Scott, Ian C. Josselyn, Sheena A. Frankland, Paul W. |
author_facet | Kenney, Justin W. Scott, Ian C. Josselyn, Sheena A. Frankland, Paul W. |
author_sort | Kenney, Justin W. |
collection | PubMed |
description | Zebrafish are a genetically tractable vertebrate that hold considerable promise for elucidating the molecular basis of behavior. Although numerous recent advances have been made in the ability to precisely manipulate the zebrafish genome, much less is known about many aspects of learning and memory in adult fish. Here, we describe the development of a contextual fear conditioning paradigm using an electric shock as the aversive stimulus. We find that contextual fear conditioning is modulated by shock intensity, prevented by an established amnestic agent (MK-801), lasts at least 14 d, and exhibits extinction. Furthermore, fish of various background strains (AB, Tu, and TL) are able to acquire fear conditioning, but differ in fear extinction rates. Taken together, we find that contextual fear conditioning in zebrafish shares many similarities with the widely used contextual fear conditioning paradigm in rodents. Combined with the amenability of genetic manipulation in zebrafish, we anticipate that our paradigm will prove to be a useful complementary system in which to examine the molecular basis of vertebrate learning and memory. |
format | Online Article Text |
id | pubmed-5602349 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-56023492018-10-01 Contextual fear conditioning in zebrafish Kenney, Justin W. Scott, Ian C. Josselyn, Sheena A. Frankland, Paul W. Learn Mem Research Zebrafish are a genetically tractable vertebrate that hold considerable promise for elucidating the molecular basis of behavior. Although numerous recent advances have been made in the ability to precisely manipulate the zebrafish genome, much less is known about many aspects of learning and memory in adult fish. Here, we describe the development of a contextual fear conditioning paradigm using an electric shock as the aversive stimulus. We find that contextual fear conditioning is modulated by shock intensity, prevented by an established amnestic agent (MK-801), lasts at least 14 d, and exhibits extinction. Furthermore, fish of various background strains (AB, Tu, and TL) are able to acquire fear conditioning, but differ in fear extinction rates. Taken together, we find that contextual fear conditioning in zebrafish shares many similarities with the widely used contextual fear conditioning paradigm in rodents. Combined with the amenability of genetic manipulation in zebrafish, we anticipate that our paradigm will prove to be a useful complementary system in which to examine the molecular basis of vertebrate learning and memory. Cold Spring Harbor Laboratory Press 2017-10 /pmc/articles/PMC5602349/ /pubmed/28916626 http://dx.doi.org/10.1101/lm.045690.117 Text en © 2017 Kenney et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first 12 months after the full-issue publication date (see http://learnmem.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Research Kenney, Justin W. Scott, Ian C. Josselyn, Sheena A. Frankland, Paul W. Contextual fear conditioning in zebrafish |
title | Contextual fear conditioning in zebrafish |
title_full | Contextual fear conditioning in zebrafish |
title_fullStr | Contextual fear conditioning in zebrafish |
title_full_unstemmed | Contextual fear conditioning in zebrafish |
title_short | Contextual fear conditioning in zebrafish |
title_sort | contextual fear conditioning in zebrafish |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602349/ https://www.ncbi.nlm.nih.gov/pubmed/28916626 http://dx.doi.org/10.1101/lm.045690.117 |
work_keys_str_mv | AT kenneyjustinw contextualfearconditioninginzebrafish AT scottianc contextualfearconditioninginzebrafish AT josselynsheenaa contextualfearconditioninginzebrafish AT franklandpaulw contextualfearconditioninginzebrafish |