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Contextual fear conditioning in zebrafish

Zebrafish are a genetically tractable vertebrate that hold considerable promise for elucidating the molecular basis of behavior. Although numerous recent advances have been made in the ability to precisely manipulate the zebrafish genome, much less is known about many aspects of learning and memory...

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Detalles Bibliográficos
Autores principales: Kenney, Justin W., Scott, Ian C., Josselyn, Sheena A., Frankland, Paul W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602349/
https://www.ncbi.nlm.nih.gov/pubmed/28916626
http://dx.doi.org/10.1101/lm.045690.117
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author Kenney, Justin W.
Scott, Ian C.
Josselyn, Sheena A.
Frankland, Paul W.
author_facet Kenney, Justin W.
Scott, Ian C.
Josselyn, Sheena A.
Frankland, Paul W.
author_sort Kenney, Justin W.
collection PubMed
description Zebrafish are a genetically tractable vertebrate that hold considerable promise for elucidating the molecular basis of behavior. Although numerous recent advances have been made in the ability to precisely manipulate the zebrafish genome, much less is known about many aspects of learning and memory in adult fish. Here, we describe the development of a contextual fear conditioning paradigm using an electric shock as the aversive stimulus. We find that contextual fear conditioning is modulated by shock intensity, prevented by an established amnestic agent (MK-801), lasts at least 14 d, and exhibits extinction. Furthermore, fish of various background strains (AB, Tu, and TL) are able to acquire fear conditioning, but differ in fear extinction rates. Taken together, we find that contextual fear conditioning in zebrafish shares many similarities with the widely used contextual fear conditioning paradigm in rodents. Combined with the amenability of genetic manipulation in zebrafish, we anticipate that our paradigm will prove to be a useful complementary system in which to examine the molecular basis of vertebrate learning and memory.
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spelling pubmed-56023492018-10-01 Contextual fear conditioning in zebrafish Kenney, Justin W. Scott, Ian C. Josselyn, Sheena A. Frankland, Paul W. Learn Mem Research Zebrafish are a genetically tractable vertebrate that hold considerable promise for elucidating the molecular basis of behavior. Although numerous recent advances have been made in the ability to precisely manipulate the zebrafish genome, much less is known about many aspects of learning and memory in adult fish. Here, we describe the development of a contextual fear conditioning paradigm using an electric shock as the aversive stimulus. We find that contextual fear conditioning is modulated by shock intensity, prevented by an established amnestic agent (MK-801), lasts at least 14 d, and exhibits extinction. Furthermore, fish of various background strains (AB, Tu, and TL) are able to acquire fear conditioning, but differ in fear extinction rates. Taken together, we find that contextual fear conditioning in zebrafish shares many similarities with the widely used contextual fear conditioning paradigm in rodents. Combined with the amenability of genetic manipulation in zebrafish, we anticipate that our paradigm will prove to be a useful complementary system in which to examine the molecular basis of vertebrate learning and memory. Cold Spring Harbor Laboratory Press 2017-10 /pmc/articles/PMC5602349/ /pubmed/28916626 http://dx.doi.org/10.1101/lm.045690.117 Text en © 2017 Kenney et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first 12 months after the full-issue publication date (see http://learnmem.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research
Kenney, Justin W.
Scott, Ian C.
Josselyn, Sheena A.
Frankland, Paul W.
Contextual fear conditioning in zebrafish
title Contextual fear conditioning in zebrafish
title_full Contextual fear conditioning in zebrafish
title_fullStr Contextual fear conditioning in zebrafish
title_full_unstemmed Contextual fear conditioning in zebrafish
title_short Contextual fear conditioning in zebrafish
title_sort contextual fear conditioning in zebrafish
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602349/
https://www.ncbi.nlm.nih.gov/pubmed/28916626
http://dx.doi.org/10.1101/lm.045690.117
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