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Target-triggered cascade assembly of a catalytic network as an artificial enzyme for highly efficient sensing
Determining the catalytic activity of artificial enzymes is an ongoing challenge. In this work, we design a porphyrin-based enzymatic network through the target-triggered cascade assembly of catalytic nanoparticles. The nanoparticles are synthesized via the covalent binding of hemin to amino-coated...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602372/ https://www.ncbi.nlm.nih.gov/pubmed/28959405 http://dx.doi.org/10.1039/c7sc01453h |
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author | Zhang, Lei Ma, Fengjiao Lei, Jianping Liu, Jintong Ju, Huangxian |
author_facet | Zhang, Lei Ma, Fengjiao Lei, Jianping Liu, Jintong Ju, Huangxian |
author_sort | Zhang, Lei |
collection | PubMed |
description | Determining the catalytic activity of artificial enzymes is an ongoing challenge. In this work, we design a porphyrin-based enzymatic network through the target-triggered cascade assembly of catalytic nanoparticles. The nanoparticles are synthesized via the covalent binding of hemin to amino-coated gold nanoparticles and then the axial coordination of the Fe center with a dual-functional imidazole or pyridine derivative. The network, which is specifically formed by coordination polymerization triggered by Hg(2+) as the target, shows high catalytic activity due to the triple amplification of enzymatic activity during the cascade assembly. The catalytic dynamics are comparable to those of natural horseradish peroxidase. The catalytic characteristics can be ultrasensitively regulated by the target, leading to a selective methodology for the analysis of sub-attomolar Hg(2+). It has also been used for “signal-on” imaging of reactive oxygen species in living cells. This work provides a new avenue for the design of enzyme mimics, and a powerful biocatalyst with signal switching for the development of biosensing protocols. |
format | Online Article Text |
id | pubmed-5602372 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-56023722017-09-28 Target-triggered cascade assembly of a catalytic network as an artificial enzyme for highly efficient sensing Zhang, Lei Ma, Fengjiao Lei, Jianping Liu, Jintong Ju, Huangxian Chem Sci Chemistry Determining the catalytic activity of artificial enzymes is an ongoing challenge. In this work, we design a porphyrin-based enzymatic network through the target-triggered cascade assembly of catalytic nanoparticles. The nanoparticles are synthesized via the covalent binding of hemin to amino-coated gold nanoparticles and then the axial coordination of the Fe center with a dual-functional imidazole or pyridine derivative. The network, which is specifically formed by coordination polymerization triggered by Hg(2+) as the target, shows high catalytic activity due to the triple amplification of enzymatic activity during the cascade assembly. The catalytic dynamics are comparable to those of natural horseradish peroxidase. The catalytic characteristics can be ultrasensitively regulated by the target, leading to a selective methodology for the analysis of sub-attomolar Hg(2+). It has also been used for “signal-on” imaging of reactive oxygen species in living cells. This work provides a new avenue for the design of enzyme mimics, and a powerful biocatalyst with signal switching for the development of biosensing protocols. Royal Society of Chemistry 2017-07-01 2017-04-28 /pmc/articles/PMC5602372/ /pubmed/28959405 http://dx.doi.org/10.1039/c7sc01453h Text en This journal is © The Royal Society of Chemistry 2017 https://creativecommons.org/licenses/by-nc/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Chemistry Zhang, Lei Ma, Fengjiao Lei, Jianping Liu, Jintong Ju, Huangxian Target-triggered cascade assembly of a catalytic network as an artificial enzyme for highly efficient sensing |
title | Target-triggered cascade assembly of a catalytic network as an artificial enzyme for highly efficient sensing
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title_full | Target-triggered cascade assembly of a catalytic network as an artificial enzyme for highly efficient sensing
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title_fullStr | Target-triggered cascade assembly of a catalytic network as an artificial enzyme for highly efficient sensing
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title_full_unstemmed | Target-triggered cascade assembly of a catalytic network as an artificial enzyme for highly efficient sensing
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title_short | Target-triggered cascade assembly of a catalytic network as an artificial enzyme for highly efficient sensing
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title_sort | target-triggered cascade assembly of a catalytic network as an artificial enzyme for highly efficient sensing |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602372/ https://www.ncbi.nlm.nih.gov/pubmed/28959405 http://dx.doi.org/10.1039/c7sc01453h |
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