Cargando…
Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo
Bacterial infection is a major hurdle to wound healing, and the overuse of antibiotics have led to global issue, such as emergence of multidrug-resistant bacteria, even “super bacteria”. On the contrary, nanosilver (NS) can kill bacteria without causing resistant bacterial strains. In this study, NS...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602461/ https://www.ncbi.nlm.nih.gov/pubmed/28979121 http://dx.doi.org/10.2147/IJN.S140648 |
_version_ | 1783264572414623744 |
---|---|
author | Liu, Menglong Luo, Gaoxing Wang, Ying He, Weifeng Liu, Tengfei Zhou, Daijun Hu, Xiaohong Xing, Malcolm Wu, Jun |
author_facet | Liu, Menglong Luo, Gaoxing Wang, Ying He, Weifeng Liu, Tengfei Zhou, Daijun Hu, Xiaohong Xing, Malcolm Wu, Jun |
author_sort | Liu, Menglong |
collection | PubMed |
description | Bacterial infection is a major hurdle to wound healing, and the overuse of antibiotics have led to global issue, such as emergence of multidrug-resistant bacteria, even “super bacteria”. On the contrary, nanosilver (NS) can kill bacteria without causing resistant bacterial strains. In this study, NS was simply generated in situ on the polycaprolactone (PCL) nanofibrous mesh using an environmentally benign and mussel-inspired dopamine (DA). Scanning electron microscopy showed that NS uniformly formed on the nanofibers of PCL mesh. Fourier transform infrared spectroscopy revealed the step-by-step preparation of pristine PCL mesh, including DA coating and NS formation, which were further verified by water contact angle changing from hydrophobic to hydrophilic. To optimize the NS dose, the antibacterial activity of PCL/NS against Staphylococcus aureus, Escherichia coli and Acinetobacter baumannii was detected by bacterial suspension assay, and the cytotoxicity of NS was evaluated using cellular morphology observation and Cell Counting Kit-8 (CCK8) assay. Then, inductively coupled plasma atomic emission spectrometry exhibited that the optimized PCL/NS had a safe and sustained silver release. Moreover, PCL/NS could effectively inhibit bacterial infection in an infectious murine full-thickness skin wound model. As demonstrated by the enhanced level of proliferating cell nuclear antigen (PCNA) in keratinocytes and longer length of neo-formed epidermis, PCL/NS accelerated wound healing by promoting re-epithelialization via enhancing keratinocyte proliferation in infectious wounds. |
format | Online Article Text |
id | pubmed-5602461 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-56024612017-10-04 Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo Liu, Menglong Luo, Gaoxing Wang, Ying He, Weifeng Liu, Tengfei Zhou, Daijun Hu, Xiaohong Xing, Malcolm Wu, Jun Int J Nanomedicine Original Research Bacterial infection is a major hurdle to wound healing, and the overuse of antibiotics have led to global issue, such as emergence of multidrug-resistant bacteria, even “super bacteria”. On the contrary, nanosilver (NS) can kill bacteria without causing resistant bacterial strains. In this study, NS was simply generated in situ on the polycaprolactone (PCL) nanofibrous mesh using an environmentally benign and mussel-inspired dopamine (DA). Scanning electron microscopy showed that NS uniformly formed on the nanofibers of PCL mesh. Fourier transform infrared spectroscopy revealed the step-by-step preparation of pristine PCL mesh, including DA coating and NS formation, which were further verified by water contact angle changing from hydrophobic to hydrophilic. To optimize the NS dose, the antibacterial activity of PCL/NS against Staphylococcus aureus, Escherichia coli and Acinetobacter baumannii was detected by bacterial suspension assay, and the cytotoxicity of NS was evaluated using cellular morphology observation and Cell Counting Kit-8 (CCK8) assay. Then, inductively coupled plasma atomic emission spectrometry exhibited that the optimized PCL/NS had a safe and sustained silver release. Moreover, PCL/NS could effectively inhibit bacterial infection in an infectious murine full-thickness skin wound model. As demonstrated by the enhanced level of proliferating cell nuclear antigen (PCNA) in keratinocytes and longer length of neo-formed epidermis, PCL/NS accelerated wound healing by promoting re-epithelialization via enhancing keratinocyte proliferation in infectious wounds. Dove Medical Press 2017-09-12 /pmc/articles/PMC5602461/ /pubmed/28979121 http://dx.doi.org/10.2147/IJN.S140648 Text en © 2017 Liu et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Liu, Menglong Luo, Gaoxing Wang, Ying He, Weifeng Liu, Tengfei Zhou, Daijun Hu, Xiaohong Xing, Malcolm Wu, Jun Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo |
title | Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo |
title_full | Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo |
title_fullStr | Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo |
title_full_unstemmed | Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo |
title_short | Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo |
title_sort | optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602461/ https://www.ncbi.nlm.nih.gov/pubmed/28979121 http://dx.doi.org/10.2147/IJN.S140648 |
work_keys_str_mv | AT liumenglong optimizationandintegrationofnanosilveronpolycaprolactonenanofibrousmeshforbacterialinhibitionandwoundhealinginvitroandinvivo AT luogaoxing optimizationandintegrationofnanosilveronpolycaprolactonenanofibrousmeshforbacterialinhibitionandwoundhealinginvitroandinvivo AT wangying optimizationandintegrationofnanosilveronpolycaprolactonenanofibrousmeshforbacterialinhibitionandwoundhealinginvitroandinvivo AT heweifeng optimizationandintegrationofnanosilveronpolycaprolactonenanofibrousmeshforbacterialinhibitionandwoundhealinginvitroandinvivo AT liutengfei optimizationandintegrationofnanosilveronpolycaprolactonenanofibrousmeshforbacterialinhibitionandwoundhealinginvitroandinvivo AT zhoudaijun optimizationandintegrationofnanosilveronpolycaprolactonenanofibrousmeshforbacterialinhibitionandwoundhealinginvitroandinvivo AT huxiaohong optimizationandintegrationofnanosilveronpolycaprolactonenanofibrousmeshforbacterialinhibitionandwoundhealinginvitroandinvivo AT xingmalcolm optimizationandintegrationofnanosilveronpolycaprolactonenanofibrousmeshforbacterialinhibitionandwoundhealinginvitroandinvivo AT wujun optimizationandintegrationofnanosilveronpolycaprolactonenanofibrousmeshforbacterialinhibitionandwoundhealinginvitroandinvivo |