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Expression and Functional Characterization of the BNIP3 Protein in Renal Cell Carcinomas
BNIP3 (Bcl-2/adenovirus E1B 19-kDa interacting protein 3) is a BH3-only protein that regulates apoptosis and autophagy. BNIP3 plays also an important role in hypoxia-induced cell response and is regulated by HIF1. Here, we studied a possible association of BNIP3 expression and the prognosis of patie...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Neoplasia Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602480/ https://www.ncbi.nlm.nih.gov/pubmed/28918350 http://dx.doi.org/10.1016/j.tranon.2017.08.008 |
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author | Macher-Goeppinger, Stephan Keith, Martina Hatiboglu, Gencay Hohenfellner, Markus Schirmacher, Peter Roth, Wilfried Tagscherer, Katrin E. |
author_facet | Macher-Goeppinger, Stephan Keith, Martina Hatiboglu, Gencay Hohenfellner, Markus Schirmacher, Peter Roth, Wilfried Tagscherer, Katrin E. |
author_sort | Macher-Goeppinger, Stephan |
collection | PubMed |
description | BNIP3 (Bcl-2/adenovirus E1B 19-kDa interacting protein 3) is a BH3-only protein that regulates apoptosis and autophagy. BNIP3 plays also an important role in hypoxia-induced cell response and is regulated by HIF1. Here, we studied a possible association of BNIP3 expression and the prognosis of patients with renal cell carcinomas (RCCs) and examined the functional relevance of BNIP3 in the regulation of cell survival and apoptosis of renal carcinoma cells. BNIP3 expression was determined by immunohistochemistry in RCC tumor tissue samples of 569 patients using a tissue microarray. Functional characterization of BNIP3 in renal carcinoma cells indicates prosurvival effects. In human RCC tumor samples, high cytoplasmic BNIP3 expression was associated with high-grade RCCs and regional lymph node metastasis. BNIP3 expression correlated negatively with disease-specific survival. Multivariate Cox regression analysis retained BNIP3 expression as an independent prognostic factor in patients without distant metastasis. Together, our studies imply that BNIP3 regulates cell survival in RCCs and its expression is an independent prognostic marker in patients with localized RCCs. |
format | Online Article Text |
id | pubmed-5602480 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Neoplasia Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-56024802017-09-25 Expression and Functional Characterization of the BNIP3 Protein in Renal Cell Carcinomas Macher-Goeppinger, Stephan Keith, Martina Hatiboglu, Gencay Hohenfellner, Markus Schirmacher, Peter Roth, Wilfried Tagscherer, Katrin E. Transl Oncol Original article BNIP3 (Bcl-2/adenovirus E1B 19-kDa interacting protein 3) is a BH3-only protein that regulates apoptosis and autophagy. BNIP3 plays also an important role in hypoxia-induced cell response and is regulated by HIF1. Here, we studied a possible association of BNIP3 expression and the prognosis of patients with renal cell carcinomas (RCCs) and examined the functional relevance of BNIP3 in the regulation of cell survival and apoptosis of renal carcinoma cells. BNIP3 expression was determined by immunohistochemistry in RCC tumor tissue samples of 569 patients using a tissue microarray. Functional characterization of BNIP3 in renal carcinoma cells indicates prosurvival effects. In human RCC tumor samples, high cytoplasmic BNIP3 expression was associated with high-grade RCCs and regional lymph node metastasis. BNIP3 expression correlated negatively with disease-specific survival. Multivariate Cox regression analysis retained BNIP3 expression as an independent prognostic factor in patients without distant metastasis. Together, our studies imply that BNIP3 regulates cell survival in RCCs and its expression is an independent prognostic marker in patients with localized RCCs. Neoplasia Press 2017-09-15 /pmc/articles/PMC5602480/ /pubmed/28918350 http://dx.doi.org/10.1016/j.tranon.2017.08.008 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original article Macher-Goeppinger, Stephan Keith, Martina Hatiboglu, Gencay Hohenfellner, Markus Schirmacher, Peter Roth, Wilfried Tagscherer, Katrin E. Expression and Functional Characterization of the BNIP3 Protein in Renal Cell Carcinomas |
title | Expression and Functional Characterization of the BNIP3 Protein in Renal Cell Carcinomas |
title_full | Expression and Functional Characterization of the BNIP3 Protein in Renal Cell Carcinomas |
title_fullStr | Expression and Functional Characterization of the BNIP3 Protein in Renal Cell Carcinomas |
title_full_unstemmed | Expression and Functional Characterization of the BNIP3 Protein in Renal Cell Carcinomas |
title_short | Expression and Functional Characterization of the BNIP3 Protein in Renal Cell Carcinomas |
title_sort | expression and functional characterization of the bnip3 protein in renal cell carcinomas |
topic | Original article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602480/ https://www.ncbi.nlm.nih.gov/pubmed/28918350 http://dx.doi.org/10.1016/j.tranon.2017.08.008 |
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