High-throughput Nuclear Delivery and Rapid Expression of DNA via Mechanical and Electrical Cell-Membrane Disruption

Nuclear transfection of DNA into mammalian cells is challenging yet critical for many biological and medical studies. Here, by combining cell squeezing and electric-field-driven transport in a device that integrates microfluidic channels with constrictions and microelectrodes, we demonstrate nuclear...

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Detalles Bibliográficos
Autores principales: Ding, Xiaoyun, Stewart, Martin, Sharei, Armon, Weaver, James C., Langer, Robert S., Jensen, Klavs F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602535/
https://www.ncbi.nlm.nih.gov/pubmed/28932622
http://dx.doi.org/10.1038/s41551-017-0039

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602535/
https://www.ncbi.nlm.nih.gov/pubmed/28932622
http://dx.doi.org/10.1038/s41551-017-0039

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