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The role of the PI3K-Akt signaling pathway in the developmental competence of bovine oocytes

The ovarian follicle encloses oocytes in a microenvironment throughout their growth and acquisition of competence. Evidence suggests a dynamic interplay among follicular cells and oocytes, since they are constantly exchanging “messages”. We dissected bovine ovarian follicles and recovered follicular...

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Detalles Bibliográficos
Autores principales: Andrade, Gabriella Mamede, da Silveira, Juliano Coelho, Perrini, Claudia, Del Collado, Maite, Gebremedhn, Samuel, Tesfaye, Dawit, Meirelles, Flávio Vieira, Perecin, Felipe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602670/
https://www.ncbi.nlm.nih.gov/pubmed/28922408
http://dx.doi.org/10.1371/journal.pone.0185045
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author Andrade, Gabriella Mamede
da Silveira, Juliano Coelho
Perrini, Claudia
Del Collado, Maite
Gebremedhn, Samuel
Tesfaye, Dawit
Meirelles, Flávio Vieira
Perecin, Felipe
author_facet Andrade, Gabriella Mamede
da Silveira, Juliano Coelho
Perrini, Claudia
Del Collado, Maite
Gebremedhn, Samuel
Tesfaye, Dawit
Meirelles, Flávio Vieira
Perecin, Felipe
author_sort Andrade, Gabriella Mamede
collection PubMed
description The ovarian follicle encloses oocytes in a microenvironment throughout their growth and acquisition of competence. Evidence suggests a dynamic interplay among follicular cells and oocytes, since they are constantly exchanging “messages”. We dissected bovine ovarian follicles and recovered follicular cells (FCs—granulosa and cumulus cells) and cumulus-oocyte complexes (COCs) to investigate whether the PI3K-Akt signaling pathway impacted oocyte quality. Following follicle rupture, COCs were individually selected for in vitro cultures to track the follicular cells based on oocyte competence to reach the blastocyst stage after parthenogenetic activation. Levels of PI3K-Akt signaling pathway components in FCs correlated with oocyte competence. This pathway is upregulated in FCs from follicles with high-quality oocytes that are able to reach the blastocyst stage, as indicated by decreased levels of PTEN and increased levels of the PTEN regulators bta-miR-494 and bta-miR-20a. Using PI3K-Akt responsive genes, we showed decreased FOXO3a levels and BAX levels in lower quality groups, indicating changes in cell cycle progression, oxidative response and apoptosis. Based on these results, the measurement of levels of PI3K-Akt pathway components in FCs from ovarian follicles carrying oocytes with distinct developmental competences is a useful tool to identify putative molecular pathways involved in the acquisition of oocyte competence.
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spelling pubmed-56026702017-09-22 The role of the PI3K-Akt signaling pathway in the developmental competence of bovine oocytes Andrade, Gabriella Mamede da Silveira, Juliano Coelho Perrini, Claudia Del Collado, Maite Gebremedhn, Samuel Tesfaye, Dawit Meirelles, Flávio Vieira Perecin, Felipe PLoS One Research Article The ovarian follicle encloses oocytes in a microenvironment throughout their growth and acquisition of competence. Evidence suggests a dynamic interplay among follicular cells and oocytes, since they are constantly exchanging “messages”. We dissected bovine ovarian follicles and recovered follicular cells (FCs—granulosa and cumulus cells) and cumulus-oocyte complexes (COCs) to investigate whether the PI3K-Akt signaling pathway impacted oocyte quality. Following follicle rupture, COCs were individually selected for in vitro cultures to track the follicular cells based on oocyte competence to reach the blastocyst stage after parthenogenetic activation. Levels of PI3K-Akt signaling pathway components in FCs correlated with oocyte competence. This pathway is upregulated in FCs from follicles with high-quality oocytes that are able to reach the blastocyst stage, as indicated by decreased levels of PTEN and increased levels of the PTEN regulators bta-miR-494 and bta-miR-20a. Using PI3K-Akt responsive genes, we showed decreased FOXO3a levels and BAX levels in lower quality groups, indicating changes in cell cycle progression, oxidative response and apoptosis. Based on these results, the measurement of levels of PI3K-Akt pathway components in FCs from ovarian follicles carrying oocytes with distinct developmental competences is a useful tool to identify putative molecular pathways involved in the acquisition of oocyte competence. Public Library of Science 2017-09-18 /pmc/articles/PMC5602670/ /pubmed/28922408 http://dx.doi.org/10.1371/journal.pone.0185045 Text en © 2017 Andrade et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Andrade, Gabriella Mamede
da Silveira, Juliano Coelho
Perrini, Claudia
Del Collado, Maite
Gebremedhn, Samuel
Tesfaye, Dawit
Meirelles, Flávio Vieira
Perecin, Felipe
The role of the PI3K-Akt signaling pathway in the developmental competence of bovine oocytes
title The role of the PI3K-Akt signaling pathway in the developmental competence of bovine oocytes
title_full The role of the PI3K-Akt signaling pathway in the developmental competence of bovine oocytes
title_fullStr The role of the PI3K-Akt signaling pathway in the developmental competence of bovine oocytes
title_full_unstemmed The role of the PI3K-Akt signaling pathway in the developmental competence of bovine oocytes
title_short The role of the PI3K-Akt signaling pathway in the developmental competence of bovine oocytes
title_sort role of the pi3k-akt signaling pathway in the developmental competence of bovine oocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602670/
https://www.ncbi.nlm.nih.gov/pubmed/28922408
http://dx.doi.org/10.1371/journal.pone.0185045
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