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The novel protein C3orf43 accelerates hepatocyte proliferation
BACKGROUND: Our previous study found that single-pass membrane protein with coiled-coil domains 1 (C3orf43; XM_006248472.3) was significantly upregulated in the proliferative phase during liver regeneration. This indicates that C3orf43 plays a vital role in liver cell proliferation. However, its phy...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603091/ https://www.ncbi.nlm.nih.gov/pubmed/28932249 http://dx.doi.org/10.1186/s11658-017-0051-3 |
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author | Zhang, Chunyan Chang, Cuifang Li, Deming Zhang, Fuchun Xu, Cunshuan |
author_facet | Zhang, Chunyan Chang, Cuifang Li, Deming Zhang, Fuchun Xu, Cunshuan |
author_sort | Zhang, Chunyan |
collection | PubMed |
description | BACKGROUND: Our previous study found that single-pass membrane protein with coiled-coil domains 1 (C3orf43; XM_006248472.3) was significantly upregulated in the proliferative phase during liver regeneration. This indicates that C3orf43 plays a vital role in liver cell proliferation. However, its physiological functions remains unclear. METHODS: The expressions of C3orf43 in BRL-3A cells transfected with C3orf43-siRNA (C3-siRNA) or overexpressing the vector plasmid pCDH-C3orf43 (pCDH-C3) were measured via RT-qPCR and western blot. Cell growth and proliferation were determined using MTT and flow cytometry. Cell proliferation-related gene expression was measured using RT-qPCR and western blot. RESULTS: It was found that upregulation of C3orf43 by pCDH-C3 promoted hepatocyte proliferation, and inhibition of C3orf43 by C3-siRNA led to the reduction of cell proliferation. The results of qRT-PCR and western blot assay showed that the C3-siRNA group downregulated the expression of cell proliferation-related genes like JUN, MYC, CCND1 and CCNA2, and the pCDH-C3 group upregulated the expression of those genes. CONCLUSION: These findings reveal that C3orf43 may contribute to hepatocyte proliferation and may have the potential to promote liver repair and regeneration. |
format | Online Article Text |
id | pubmed-5603091 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-56030912017-09-20 The novel protein C3orf43 accelerates hepatocyte proliferation Zhang, Chunyan Chang, Cuifang Li, Deming Zhang, Fuchun Xu, Cunshuan Cell Mol Biol Lett Short Report BACKGROUND: Our previous study found that single-pass membrane protein with coiled-coil domains 1 (C3orf43; XM_006248472.3) was significantly upregulated in the proliferative phase during liver regeneration. This indicates that C3orf43 plays a vital role in liver cell proliferation. However, its physiological functions remains unclear. METHODS: The expressions of C3orf43 in BRL-3A cells transfected with C3orf43-siRNA (C3-siRNA) or overexpressing the vector plasmid pCDH-C3orf43 (pCDH-C3) were measured via RT-qPCR and western blot. Cell growth and proliferation were determined using MTT and flow cytometry. Cell proliferation-related gene expression was measured using RT-qPCR and western blot. RESULTS: It was found that upregulation of C3orf43 by pCDH-C3 promoted hepatocyte proliferation, and inhibition of C3orf43 by C3-siRNA led to the reduction of cell proliferation. The results of qRT-PCR and western blot assay showed that the C3-siRNA group downregulated the expression of cell proliferation-related genes like JUN, MYC, CCND1 and CCNA2, and the pCDH-C3 group upregulated the expression of those genes. CONCLUSION: These findings reveal that C3orf43 may contribute to hepatocyte proliferation and may have the potential to promote liver repair and regeneration. BioMed Central 2017-09-16 /pmc/articles/PMC5603091/ /pubmed/28932249 http://dx.doi.org/10.1186/s11658-017-0051-3 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Short Report Zhang, Chunyan Chang, Cuifang Li, Deming Zhang, Fuchun Xu, Cunshuan The novel protein C3orf43 accelerates hepatocyte proliferation |
title | The novel protein C3orf43 accelerates hepatocyte proliferation |
title_full | The novel protein C3orf43 accelerates hepatocyte proliferation |
title_fullStr | The novel protein C3orf43 accelerates hepatocyte proliferation |
title_full_unstemmed | The novel protein C3orf43 accelerates hepatocyte proliferation |
title_short | The novel protein C3orf43 accelerates hepatocyte proliferation |
title_sort | novel protein c3orf43 accelerates hepatocyte proliferation |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603091/ https://www.ncbi.nlm.nih.gov/pubmed/28932249 http://dx.doi.org/10.1186/s11658-017-0051-3 |
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